Line 34: | Line 34: | ||
<span><img style="height:310px; width: 280px; float:right" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png"/> | <span><img style="height:310px; width: 280px; float:right" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png"/> | ||
<br><i></i></span> | <br><i></i></span> | ||
− | + | <br><br><br> | |
<span class="stitle">Bba_K1879000</span><br><br> | <span class="stitle">Bba_K1879000</span><br><br> | ||
<p><span class="p"> | <p><span class="p"> |
Revision as of 21:28, 16 October 2016
![](https://static.igem.org/mediawiki/2016/9/93/T--Virginia--Parts_header.png)
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
![](https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png)
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et al (Ref), and we included an RBS sequence upstream of the coding sequence.
![](https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png)
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage
enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our
biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce
wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively
cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.