Difference between revisions of "Team:Pasteur Paris/Protocol"
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<td><strong>Digestion</strong></td> | <td><strong>Digestion</strong></td> | ||
<td>To linearize the different plasmids with appropriate enzymes. Perform restriction enzyme digestion in order to recover linear backbones of the plasmids. And chose the appropriate restriction sites based on the host plasmids. </td> | <td>To linearize the different plasmids with appropriate enzymes. Perform restriction enzyme digestion in order to recover linear backbones of the plasmids. And chose the appropriate restriction sites based on the host plasmids. </td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/a/ab/T--Pasteur_Paris--Restriction_digestion_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
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<td>To reduce vector self-ligation and favor that of vector to insert instead. | <td>To reduce vector self-ligation and favor that of vector to insert instead. | ||
</td> | </td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/c/c0/T--Pasteur_Paris--dephosphorylation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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<td>To link an insert to its host plasmid before the transformation. This step happens after the digestion of the insert and the plasmid with the same restriction sites enzymes. | <td>To link an insert to its host plasmid before the transformation. This step happens after the digestion of the insert and the plasmid with the same restriction sites enzymes. | ||
</td> | </td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/6/6f/T--Pasteur_Paris--Ligation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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<td><strong>Electrophoresis</strong></td> | <td><strong>Electrophoresis</strong></td> | ||
<td>To know the size of DNA fragments to check the efficiency of a digestion for instance.</td> | <td>To know the size of DNA fragments to check the efficiency of a digestion for instance.</td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/6/60/T--Pasteur_Paris--Gel_electrophoresis_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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<td><strong>Gel extraction kit</strong></td> | <td><strong>Gel extraction kit</strong></td> | ||
<td>To get back the DNA purified thanks to the electrophoresis on agarose gel.</td> | <td>To get back the DNA purified thanks to the electrophoresis on agarose gel.</td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4e/T--Pasteur_Paris--Gel_extraction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td><strong>Transformation</strong></td> | <td><strong>Transformation</strong></td> | ||
<td>To increase the amount of plasmid by transformation in competent cells. The amount of plasmid supplied is insufficient to perform all our future experiments.</td> | <td>To increase the amount of plasmid by transformation in competent cells. The amount of plasmid supplied is insufficient to perform all our future experiments.</td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/c/ca/T--Pasteur_Paris--Transformation_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
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<td><strong>Harvest and culture</strong></td> | <td><strong>Harvest and culture</strong></td> | ||
<td>In order to obtain a large amount of plasmid, we need to grow the bacteria overnight.</td> | <td>In order to obtain a large amount of plasmid, we need to grow the bacteria overnight.</td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/4/4b/T--Pasteur_Paris--Bacterial_culture_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
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<td><strong>IPTG induction</strong></td> | <td><strong>IPTG induction</strong></td> | ||
<td>To increase the production of our protein which depends on this molecule.</td> | <td>To increase the production of our protein which depends on this molecule.</td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/a/a4/T--Pasteur_Paris--Protein_induction_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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| − | <td><strong>Purification Fast | + | <td><strong>Protein Purification Fast Protein Liquid Chromatography</strong></td> |
<td>To check if the His-tag works and if our protein has really been produced </td> | <td>To check if the His-tag works and if our protein has really been produced </td> | ||
| − | <td> <center><a href=""><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> | + | <td> <center><a href="https://static.igem.org/mediawiki/2016/0/07/T--Pasteur_Paris--FPLC_Protein_purification_protocol.pdf"><img src="https://static.igem.org/mediawiki/2016/c/c1/DownloadT--iGEM16_Pasteur_Paris--file.xxx.png" width="40%" alt=""></a></center></td> |
</tr> | </tr> | ||
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