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Revision as of 18:10, 17 October 2016
Our original experiment, which looked at making a microbial fuel cell using yeast, followed the University of Reading’s protocol kindly given to us by Dr Ed Milner, Dr Paniz Izadi and Professor Ian Head. The protocol can be seen here
Microbial Fuel Cell with E. coli and ArabinoseAfter discussing the ethical issues of using yeast with PEALS, we decided to change our constructs to E. coli. However, we generally used the same protocol as above and made only slight changes, such as using 1g of arabinose as well as the 9g of glucose dissolved in 50ml of potassium phosphate buffer. Around 4ml of the BBa_K1895004 and the BBa_K1895005 was added to individual fuel cells.
The fuel cells was left to run for an hour and the voltage was taken every 3 minutes.