Difference between revisions of "Team:Austin UTexas/Parts"

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<h4>CpxA-CpxR</h4>
 
<h4>CpxA-CpxR</h4>
<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The construct can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097000">K2097000</a>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a></p>
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<p>CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in <i>Shigella sonnei</i>, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The construct can be found on the iGEM registry as: <a href="http://parts.igem.org/Part:BBa_K2097000">K2097000</a>, as well as on our results page <a href="https://2016.igem.org/Team:Austin_UTexas/Results">here</a></p>.
 
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Revision as of 23:06, 17 October 2016

Austin_UTexas

Registry Parts


pH Sensors


CpxA-CpxR

CpxA-CpxR is a two-component mechanism that is activated at pH 7.4 and repressed at pH 6.0. CpxA is an intermembrane protein that autophosphorylates at a certain external pH, CpxR (a kinase) then gets phosphorylated by CpxA and acts as a transcription factor. This system originally is a transcription factor for the virF gene, but virF was replaced with a reporter. The original sequence was found in Shigella sonnei, but E. coli has a homolog of these proteins so all that is required on the construct is the appropriate prefix/suffix and CpxR binding site. The construct can be found on the iGEM registry as: K2097000, as well as on our results page here

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Composite Parts


Blue Chromoprotein

K2097001 is a composite part made up of BBa_K592009 (blue chromoprotein) and BBa_K608002 (a strong RBS and promoter). The protein exhibits a strong blue/purple color when expressed. BBa_K608002 is made up of a promoter, BBa_J23104, and an RBS, BBa_B003. LINKS


Yellow-Green Chromoprotein

K2097002 is a composite part made up of BBa_K1033916 (amajLime, a yellow-green chromoprotein)and BBa_K608006 (a medium promoter and RBS). LINKS