Difference between revisions of "Team:Paris Saclay/Notebook/July/1"

Line 12: Line 12:
 
! Buffer STOP (15mL)
 
! Buffer STOP (15mL)
 
|-
 
|-
−
| Na2HPO4 – 3,125g
+
|  
 +
Na2HPO4 – 3,125g
 
NaH2PO4.H2O – 1,38g
 
NaH2PO4.H2O – 1,38g
−
  KCl – 0,185g
+
KCl – 0,185g
 
   MgSO4 – 0,04g
 
   MgSO4 – 0,04g
 
   β-mercaptoethanol – 0,893mL
 
   β-mercaptoethanol – 0,893mL

Revision as of 16:16, 6 July 2016

Tuesday 1st July

Lab Work

Biobrick characterization

Buffer preparations

by Charlene and Caroline

Buffer Z (250mL) Buffer Z (250mL) Buffer STOP (15mL)

Na2HPO4 – 3,125g NaH2PO4.H2O – 1,38g KCl – 0,185g 

 MgSO4 – 0,04g
 β-mercaptoethanol – 0,893mL
Trisphosphate pH 7,8 1M – 6,25mL 
 MgCl2 2M – 1mL
 DTT 1M -0,25mL
 EDTA 0,5M – 0,5mL
 BSA – 0,25g
 Glycerol 60% - 62,5mL
 Triton 10% (100x) – 25mL
Na2CO3 1M – 0,78g