Line 250: | Line 250: | ||
• EDTA 0,1M</br> | • EDTA 0,1M</br> | ||
• PMSF (100mM)</br> | • PMSF (100mM)</br> | ||
− | • Ni 2+ solution (100mM)</br> | + | • Ni<sup>2+</sup> solution (100mM)</br> |
• Centrifuge </br> | • Centrifuge </br> | ||
• Buffer A (50mM Tris, 150mM of NaCl)</br> | • Buffer A (50mM Tris, 150mM of NaCl)</br> |