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We have examined the <a target="blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">phaCAB BioBrick</a> created by <a title="Imperial College team 2013" href="https://2013.igem.org/Team:Imperial_College/PHB_production" target="_blank">Imperial College team 2013</a>. We have characterized the relationship between the strength of the additional promoter and ribosomal binding site and the yield of plastic. In the process we have created another phaCAB construct that appears to generate a higher yield of PHB. Furthermore, we have characterized the effect of the <a target="blank" href="http://parts.igem.org/Part:BBa_K1692020">pantothenate kinase II BioBrick</a> submitted by Imperial College’s 2015 iGEM team on <i>E. coli’s</i> proteome. For more information see our <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Demonstrate">Results Page</a>.</li> | We have examined the <a target="blank" href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K1149051">phaCAB BioBrick</a> created by <a title="Imperial College team 2013" href="https://2013.igem.org/Team:Imperial_College/PHB_production" target="_blank">Imperial College team 2013</a>. We have characterized the relationship between the strength of the additional promoter and ribosomal binding site and the yield of plastic. In the process we have created another phaCAB construct that appears to generate a higher yield of PHB. Furthermore, we have characterized the effect of the <a target="blank" href="http://parts.igem.org/Part:BBa_K1692020">pantothenate kinase II BioBrick</a> submitted by Imperial College’s 2015 iGEM team on <i>E. coli’s</i> proteome. For more information see our <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Demonstrate">Results Page</a>.</li> | ||
<li><b>Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept</b><br> | <li><b>Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept</b><br> | ||
− | By producing bacteriocins and PHB from different BioBrick devices, we have demonstrated a <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Proof">functional Proof of Concept</a>. Furthermore we have now a BioBrick device that can secrete PHB. | + | By producing bacteriocins and PHB from different BioBrick devices, we have demonstrated a <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Proof">functional Proof of Concept</a>. Furthermore we have now a <a target="blank"href="http://parts.igem.org/Part:BBa_K2018050">BioBrick device</a> that can secrete PHB.</li> |
<li><b>Show your project working under real-world conditions. To achieve this criterion, you should demonstrate your whole system, or a functional proof of concept working under simulated conditions in the lab</b><br> | <li><b>Show your project working under real-world conditions. To achieve this criterion, you should demonstrate your whole system, or a functional proof of concept working under simulated conditions in the lab</b><br> | ||
Through MIC tests made with our bacteriocins, we showed their effect towards different human pathogens that are particularly complicated to treat due to the rising resistance problem. The tests showed the potential of these bacteriocins to be used in the real-world. Increasing remarkably the production of PHB by <i>E. coli</i> through implementation of secretion system, allowed us to 3D print an actual product, an implant prototype of the human mandible. Bringing us a step closer to a product that can soon reach the market. To read more about this exploit, go to <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Demonstrate">Demonstration & Results</a> and <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Proof">Proof of Concept</a>.</li> | Through MIC tests made with our bacteriocins, we showed their effect towards different human pathogens that are particularly complicated to treat due to the rising resistance problem. The tests showed the potential of these bacteriocins to be used in the real-world. Increasing remarkably the production of PHB by <i>E. coli</i> through implementation of secretion system, allowed us to 3D print an actual product, an implant prototype of the human mandible. Bringing us a step closer to a product that can soon reach the market. To read more about this exploit, go to <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Demonstrate">Demonstration & Results</a> and <a target="blank" href="https://2016.igem.org/Team:SDU-Denmark/Proof">Proof of Concept</a>.</li> |
Revision as of 19:30, 18 October 2016
Achievements
Bronze 4/4
- Register for iGEM, have a great summer, and attend the Giant Jamboree
We registered and was accepted by iGEM Headquarters on 2016-05-10 at 09:08:56. We definitely had a great summer, and we are excited to attend the Giant Jamboree. - Meet all deliverables on the Requirements page
We sure did, you are reading our wiki right now.
All our parts were registered and submitted. The safety, judging and registry form were filled as required. Also, a poster is ready and the team members are set, to present our work at the Giant Jamboree. - Clearly attribute each aspect of the project
All the help that was needed is greeted with great thankfulness under Attributions. - Document at least one new standard BioBrick Part or Device central to your project and submit this part to the iGEM Registry.
K2018030 is a secretion system for poly-beta-hydroxybutyrate (PHB). This secretion tags PHB with a hybrid protein consisting of phasin, that binds PHB and a hemolysin tag, and is recognised by a type 2 secretion system. The BioBrick also contains two transport proteins that will recognize the hybrid protein and secrete it along with PHB. This construct generates an enormous increase in PHB yield and creates the possibility for a large scale of continous production of PHB in a fed batch.
Silver 3/3
- Experimentally validate that at least one new BioBrick Part or Device of your own design and construction works as expected
Our new devices K2018014 and K2018015, are hybrid bacteriocins that have shown great effect at inhibiting growth of multi resistant Methicillin Resistant Staphylococcus aureus (MRSA) and Pseudomonas aeruginosa. The hybrids showed greater effect than a single bacteriocin protein did. Suggesting that the two bacteriocins as a hybrid have synergistic effect. - Collaboration
We have helped the following iGEM teams in different ways: The iGEM team from the Technical University of Denmark, the University of Copenhagen, the Chalmers University of Technology, Stockholm, Virginia and LMU TUM Münich. For more details, please see Collaborations. - Human practice
As it was mentioned by iGEM, this competition is much more than just sitting on the lab bench. We have not only been reaching out to highschool students at different events, but we also managed to create videos to get Denmark to know more about us and iGEM. Most importantly, we considered bioethics and philosophy of science in the methods of our project. You can read further about it under Practices and Prospects.
Gold 4/4
- Integrated human practice; Expand on your silver medal activity of human practice by demonstrating how you have integrated the investigated issues into the design and/or execution of your project
We have talked to several experts in order to develop new goals for the future perspective of our project and to expand our knowledge about the different sub elements. Among those are experts in the areas of therapeutic use of biodegradable plastic and experts in the use of antibiotics and bacteriocins as new antimicrobial components. Furthermore, we made a survey to one of the high schools we visited and used their feedback to improved our presentation and gave us new ideas for products from each sub element. We have also been talking to a nurse about what their requirements are for a patch like ours. At last, we made a survey to Danish companies that work with plastic production and investigated the possible interest in the use of plastic made by bacteria. For more information go to Integrated Human Practice or explore our website to see where it was integrated. - Improve the function OR characterization of an existing BioBrick Part or Device and enter this information in the Registry
We have examined the phaCAB BioBrick created by Imperial College team 2013. We have characterized the relationship between the strength of the additional promoter and ribosomal binding site and the yield of plastic. In the process we have created another phaCAB construct that appears to generate a higher yield of PHB. Furthermore, we have characterized the effect of the pantothenate kinase II BioBrick submitted by Imperial College’s 2015 iGEM team on E. coli’s proteome. For more information see our Results Page. - Demonstrate a functional proof of concept of your project. Your proof of concept must consist of a BioBrick device; a single BioBrick part cannot constitute a proof of concept
By producing bacteriocins and PHB from different BioBrick devices, we have demonstrated a functional Proof of Concept. Furthermore we have now a BioBrick device that can secrete PHB. - Show your project working under real-world conditions. To achieve this criterion, you should demonstrate your whole system, or a functional proof of concept working under simulated conditions in the lab
Through MIC tests made with our bacteriocins, we showed their effect towards different human pathogens that are particularly complicated to treat due to the rising resistance problem. The tests showed the potential of these bacteriocins to be used in the real-world. Increasing remarkably the production of PHB by E. coli through implementation of secretion system, allowed us to 3D print an actual product, an implant prototype of the human mandible. Bringing us a step closer to a product that can soon reach the market. To read more about this exploit, go to Demonstration & Results and Proof of Concept.
Special prizes
Education and public engagement
During the project, one of our main focuses has been to engage ourselves in the community and in discussions with whomever interested in synthetic biology. We held several talks and presentations. The audiences varied from small children at the Research day at the University of Southern Denmark, to professors in philosophy. We have taught more and learned even more. One of our engagements was being teachers for a day: we had two biotechnology classes at a local high school an entire day. We taught them about synthetic biology and made a laboratory exercise for them, which we supervised for. It was great fun. We have also focused on taking a stand for scientific reproduction, which we also have done further research about. The biotechnology classes tried to reproduce some of our protocols, which were mostly successful.
Integrated Human practice
We have talked to several experts in order to integrate new goals for the future perspective of our project and to expand our knowledge about the different sub elements. Among those are experts in the areas of therapeutic use of biodegradable plastic and experts in the use of antibiotics and bacteriocins as new antimicrobial components. We have tried to integrate some of their ideas into our project in order to make our Bacto-Aid more realistic to commercialize. The interviews have also given us reassurance of, that we are dealing with a very essential problem. We have also been talking to a nurse about what their requirements are for a patch like ours, so we would know which aspects we should focus on if hospitals were going to use our Bacto-Aid. Furthermore, we made a survey with one of the high schools we visited and used their feedback to improve the message we would like to send out. At last, we send out question forms to Danish plastic companies to hear about their possible interest in the use of plastic made by bacteria. They were very interested in our plastic, but it was important to them that the plastic price would be lower. We have therefore been working hard to optimize the production of PHB.
Best device
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