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− | <div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width: | + | <div style="float:right"><img class="mySlides" src="https://static.igem.org/mediawiki/2016/c/c5/T--Virginia--Bba_K1879000_Map.png" style="width:550px"> |
− | <img class="mySlides" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png" style="width: | + | <img class="mySlides" src="https://static.igem.org/mediawiki/2016/1/13/T--Virginia--Bba_K1879001_Map.png" style="width:550px"> |
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<span class="ptitle">Bba_K1879000</span><br><br> | <span class="ptitle">Bba_K1879000</span><br><br> | ||
<p><span class="p"> | <p><span class="p"> | ||
− | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) | + | This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ) |
− | cleavage enzyme necessary to remove the CBZ protecting group from the | + | cleavage enzyme necessary to remove the CBZ protecting group from the |
− | N-terminus of amino acids. The coding sequence was derived from Nanduri | + | N-terminus of amino acids. The coding sequence was derived from Nanduri |
et al<span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. | et al<span class="references"><sup>ref</sup><span class="refbox"><b>Reference:</b><br>Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312. | ||
</span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p> | </span></span> , and we included an RBS sequence upstream of the coding sequence.</span></p> |
Revision as of 02:23, 19 October 2016
We submitted two parts to the iGEM registry: an N-carbobenzyloxy (CBZ) cleavage enzyme part, and a composite part consisting of a promoter, the CBZ cleavage enzyme, and a terminator.
Bba_K1879000
This basic part contains the coding sequence for the N-carbobenzyloxy (CBZ)
cleavage enzyme necessary to remove the CBZ protecting group from the
N-terminus of amino acids. The coding sequence was derived from Nanduri
et alrefReference:
Nanduri, V.B., Goldberg, S., Johnston, R., and Patel, R.N. (2004). Cloning and expression of a novel enantioselective N-carbobenzyloxy-cleaving enzyme. Enzyme and Microbial Technology 34, 304–312.
, and we included an RBS sequence upstream of the coding sequence.
Bba_K1879001
This composite part contains a promoter (BBa_J23118), RBS, the CBZ cleavage enzyme, and a terminator (BBa_J61048) to make a fully functional BioBrick. In our biocontainment system, this BioBrick cleaves CBZ from N-CBZ-leucyl-tRNA to produce wild-type leucyl-tRNA. This part can be used by other iGEM teams to selectively cleave the CBZ protecting group from amino or hydroxyl groups in organic reactions.