Difference between revisions of "Team:Austin UTexas/Demonstrate"

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*Found literature describing three putative promoters in <i>Gluconobacter oxydans</i> that increase transcription under acidic conditions, and currently characterizing these sequences.
 
*Found literature describing three putative promoters in <i>Gluconobacter oxydans</i> that increase transcription under acidic conditions, and currently characterizing these sequences.
 
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<a href = "https://2016.igem.org/Team:Austin_UTexas/Results#section6">Results </a>  
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<a href = "https://2016.igem.org/Team:Austin_UTexas/Results#section6">Results </a> </html>
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<input type="image" src="https://static.igem.org/mediawiki/2016/4/40/T--Austin_UTexas--StrainNavi.png" style="width:100%"; onclick="showOne('section1')"/> <p>Kombucha Strains </p>
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<input type="image" src="https://static.igem.org/mediawiki/2016/6/64/T--Austin_UTexas--ConjugationPic.png" style="width:100%;" onclick="showOne('section2')" /><p>Conjugation </p>
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<input type="image" src="https://static.igem.org/mediawiki/2016/0/04/T--Austin_UTexas--RecapNavi.png" style="width:100%;" onclick="showOne('section3')" /><p>Recapitulation</p>
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<input type="image" src="https://static.igem.org/mediawiki/2016/b/bb/T--Austin_UTexas--EtOHNavi.png" style="width:100%;" onclick="showOne('section4')" /><p>Ethanol</p>
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<input type="image" src ="https://static.igem.org/mediawiki/2016/e/e7/T--Austin_UTexas--pHNavi.png" style="width:100%;" onclick="showOne('section6')" /><p>pH</p>
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<h2>Kombucha Strains</h2>
  
 
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*Successfully isolated microbes from various samples of kombucha.
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*Identified strains of bacteria and yeast using rRNA gene sequencing.
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*Characterized each of the isolated microbes to facilitate further experimentation.
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<h2>Recapitulation</h2>
  
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*In a process called "recapitulation," we successfully created a kombucha-like culture by adding individual strains of microbes instead of a living culture containing the entire kombucha microbiome.
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*Determined that the microbe <i>Ga. hansenii</i> is essential for the fermentation of kombucha.
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*Determined that two distinct strains of the yeast <i>Lachancea fermentati</i> are necessary for the fermentation of kombucha, including one that appears to produce high quantities of C02.
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<h2>Conjugation</h2>
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*Attempted conjugation with <i>G. oxydans</i>.
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*Performed minimum inhibitory concentration experiments between <i>G. oxydans</i> and spectinomycin, carbenicillin and kanamycin.
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*Determined that <i>G. oxydans</i> is resistant to spectinomycin and carbenicillin.
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<h2>Ethanol</h2>
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*Found literature describing sequences for genes involved in the metabolism of ethanol to acetic acid in the bacterium <i>Ga. hansenii</i>.
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*Designed Golden Gate parts for the assembly of these genes into a functional construct.
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*Used a bromothymol blue assay to compare changes in pH resulting from fermentation in multiple strains of <i>Lachancea fermentati</i> isolated from our kombucha.
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<h2>pH Sensors</h2>
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*Successfully created a neutral pH sensor with a reporter.
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*Further characterized the P-atp2 Biobrick.
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*Found literature describing three putative promoters in <i>Gluconobacter oxydans</i> that increase transcription under acidic conditions, and currently characterizing these sequences.
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Revision as of 03:53, 19 October 2016

Demonstrate


Click on one of the images below to learn more about our results!

Kombucha Strains

Conjugation

Recapitulation

Ethanol

pH