Difference between revisions of "Team:Paris Saclay/Notebook/June/21"
| Line 12: | Line 12: | ||
===Interlab study=== | ===Interlab study=== | ||
====Transformation==== | ====Transformation==== | ||
| − | '' | + | ''By Lea, Caroline and Marion'' |
50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. | 50µL of competent DH5α cells were transformed with 1µL of plasmids (for each construction: device 1, 2, 3, negative control and positive control) and one control tube was made without plasmids. Tubes were kept into ice for 30min then a thermal shock was made at 42°C for 1min. 500µL LB medium was added into each tube and incubated at 37°C for one 1h. | ||
Revision as of 13:50, 11 July 2016
