Difference between revisions of "Team:Paris Saclay/Notebook/June/23"

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''By Caroline and Lea''
 
''By Caroline and Lea''
  
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A transformation was made with Interlab study constructions using the [[Team:Paris_Saclay/Experiments#HeatShockCompetent|usual protocole]] but this time with our competent cells. A negative control and pSB1C3 blue plasmids (positive control) were also transformed and displayed.
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The cells competency was checked by transforming cells [[Team:Paris_Saclay/Experiments#HeatShockCompetent|as usual ]] with control plasmids (positive control: pSB1C3+blue; negative control).
  
 
===Interlab study===
 
===Interlab study===

Revision as of 08:35, 12 July 2016

Thursday 23rd June

Lab work

Competent cells

By Caroline and Lea

The cells competency was checked by transforming cells as usual with control plasmids (positive control: pSB1C3+blue; negative control).

Interlab study

Transformation results

By Caroline, Lea and Marion

Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.

Transformed cell cultures

By Lea

All colonies that were obtained after transformation were put into culture. One colony was taken into 3,5mL LB medium + Cm at 30µg/mL.





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