Difference between revisions of "Team:Paris Saclay/Notebook/June/23"

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''By Caroline and Lea''
 
''By Caroline and Lea''
  
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The cells competency was checked by transforming cells [[Team:Paris_Saclay/Experiments#HeatShockCompetent|as usual ]] with control plasmids (positive control: pSB1C3+blue; negative control).
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The cells competency was checked by transforming cells [[Team:Paris_Saclay/Experiments#HeatShockCompetent|as usual ]] with control plasmids.
  
 
===Interlab study===
 
===Interlab study===

Revision as of 08:38, 12 July 2016

Thursday 23rd June

Lab work

Competent cells

By Caroline and Lea

The cells competency was checked by transforming cells as usual with control plasmids.

Interlab study

Transformation results

By Caroline, Lea and Marion

Colonies were observed for all transformations except for device 1 and positive control. The transformation was redone for device 1 with 5µL and the rest of the 22/06/2016 transformation was displayed entirely.

Transformed cell cultures

By Lea

All colonies that were obtained after transformation were put into culture. One colony was taken into 3.5mL LB medium + Cm at 30µg/mL.





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