====Electrocompetents and transformation by electroporation of BL21====
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====BL21 electrocompetent cells preparation and transformation====
''By Caroline and Charlene''
''By Caroline and Charlene''
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The same protocol as 29/06/16 was used for the plasmids K1372001, K1372001+pcl_TAA and the controls which were done following the same protocol but without plasmids.
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The [[Team:Paris_Saclay/Experiments#ElectroCompetent|usual protocol]] was used for transforing cells with K1372001, K1372001+pcl_TAA and the controls which were done following the same protocol but without plasmids.
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K1372001 and the controls were streaked on LB + Cm (30µg/mL). K137200 + PCLTAA was streaked on LB + Cm (30µg/mL) + Streptomicin (50µg/mL).
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K1372001 and the controls were streaked on LB + Cm (30µg/mL). K1372001+pcl_TAA was streaked on LB + Cm (30µg/mL) + streptomicin (50µg/mL).
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This time two different streaks were made for each plasmid. The first one using 50µL from the culture. The second one was done using 100µL of each remaining culture (400µL) after concentration by centrifugation.
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This time two different streaks were made for each plasmid, the first one using 50µL from the culture, the second one with 400µL concentrated by centrifugation.
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The petri dishes made on 29/06 were stored at 4ºC
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The Petri dishes made on 29/06 were stored at 4ºC.
