Difference between revisions of "Team:Paris Saclay/Notebook/July/5"
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4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis) | 4.1 clone 6 extracted DNA (which presented a good size strip on electrophoresis) | ||
| − | 6 clones of each of the other transformations pPS16_001, pPS16_002, pPS16_003, pPS16_005 and pPS16_006 were placed in 4mL of LB added to Ampicillin (50µg/mL) at 37°C, 180RPM. For 2.2 just 2 clones were cultivated because there was not more colony. To obtain more colonies, another transformation (Heat shock competent cells, section protocols) was made with 50µL of cells, 5µL of plasmid. 50µL of transformed bacteria were displayed on LB + Ampicillin (50µg/mL)dishes. | + | 6 clones of each of the other transformations [[Team:Paris_Saclay/Notebook/June/28#pPS16_001|pPS16_001, pPS16_002, pPS16_003, pPS16_005 and pPS16_006]] were placed in 4mL of LB added to Ampicillin (50µg/mL) at 37°C, 180RPM. For 2.2 just 2 clones were cultivated because there was not more colony. To obtain more colonies, another transformation (Heat shock competent cells, section protocols) was made with 50µL of cells, 5µL of plasmid. 50µL of transformed bacteria were displayed on LB + Ampicillin (50µg/mL)dishes. |
===Biobrick characterization=== | ===Biobrick characterization=== | ||
Revision as of 14:18, 12 July 2016
