Difference between revisions of "Team:Paris Saclay/Notebook/July/6"
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=Wednesday 6<sup>th</sup> July= | =Wednesday 6<sup>th</sup> July= | ||
==Lab work== | ==Lab work== | ||
| + | <!--Rédigé par Terrence, à relire --> | ||
| + | ====Preparation of LB solid and liquid stock==== | ||
| + | ''By Léa, Naiane, Laetitia'' | ||
| + | - 2L of LB liquid : 20g/L powder LB + 2L water μQ | ||
| + | - 1L of LB solid : 1L of LB liquid +15g/L of Agar | ||
| + | The all was put in the autoclave for sterilization with the help of Sylvain. | ||
===Bringing DNA closer=== | ===Bringing DNA closer=== | ||
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===Visualization=== | ===Visualization=== | ||
| − | ==== ==== | + | ====Screen PCF for GBlock ==== |
| − | ''By '' | + | ''By Caroline'' |
| + | <!--TODO : A relire ABSOLUMENT problème de lecture et de traduction--> | ||
| + | "Megamix" were prepared for 34 tubes with 70μL of 90xTaq buffer + 70 μL of <!-- dumpamM --> + 42 μL MgCl<SUB>2</SUB> 25mM + 35 μL primer 1 + 35 μL primer 2 + 3.5 μL of Taq + 409.5 μL of water (till 700 μL completion, 35 μL corresponding to the 1 μL of liquid culture for each.) | ||
| + | |||
| + | 19 μL of mage was put into PCR's tubes then 1 μL of culture were added for each Gblock 1.1 1.2 2.1 2.2 3.1 and 3.2 and their 6 clones. (2 clnes for 2.2). | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 21:53, 17 July 2016
