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<p>In this experiment, we used 6mM of zinc sulphate (ZnSO<sub>4</sub>) and carried out a dilution of half for each column of a 96 well plate to try and identify the minimum inhibitory concentration for the <i>E. coli</i> to grow in. Cells were again placed in the pSB1C3 backbone and fresh cultures were grown at 37°C overnight. The cells were diluted down to a starting optical density of around 0.05 at 600nm using LB broth with chloramphenicol. Then, 100μl of cells were placed in the wells with the corresponding ZnSO<sub>4</sub> concentration which was diluted using LB with chloramphenicol. This experiment was left for 16 hours to give the <i>E. coli</i> time to grow and replicate, see figure 1.</p> | <p>In this experiment, we used 6mM of zinc sulphate (ZnSO<sub>4</sub>) and carried out a dilution of half for each column of a 96 well plate to try and identify the minimum inhibitory concentration for the <i>E. coli</i> to grow in. Cells were again placed in the pSB1C3 backbone and fresh cultures were grown at 37°C overnight. The cells were diluted down to a starting optical density of around 0.05 at 600nm using LB broth with chloramphenicol. Then, 100μl of cells were placed in the wells with the corresponding ZnSO<sub>4</sub> concentration which was diluted using LB with chloramphenicol. This experiment was left for 16 hours to give the <i>E. coli</i> time to grow and replicate, see figure 1.</p> | ||
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<img alt="Zinc Growth" src="https://static.igem.org/mediawiki/2016/f/f3/T--Newcastle--VR_Zinc_Growth.png"/> | <img alt="Zinc Growth" src="https://static.igem.org/mediawiki/2016/f/f3/T--Newcastle--VR_Zinc_Growth.png"/> | ||
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<p>Figure 1. The minimum inhibitory concentration for <i>E. coli</i> growing in ZnSO<sub>4</sub>. The cells were left for 16 hours to grow in different zinc sulphate concentrations with LB broth and chlormaphenicol. OD600 was measured after this 16 hour growth period.</p> | <p>Figure 1. The minimum inhibitory concentration for <i>E. coli</i> growing in ZnSO<sub>4</sub>. The cells were left for 16 hours to grow in different zinc sulphate concentrations with LB broth and chlormaphenicol. OD600 was measured after this 16 hour growth period.</p> | ||
Revision as of 17:07, 19 October 2016
Results for Arabinose controlled variable resistor
Growth in Varying ZnSO4 Concentrations (Minimum Inhibitory Concentration)
In this experiment, we used 6mM of zinc sulphate (ZnSO4) and carried out a dilution of half for each column of a 96 well plate to try and identify the minimum inhibitory concentration for the E. coli to grow in. Cells were again placed in the pSB1C3 backbone and fresh cultures were grown at 37°C overnight. The cells were diluted down to a starting optical density of around 0.05 at 600nm using LB broth with chloramphenicol. Then, 100μl of cells were placed in the wells with the corresponding ZnSO4 concentration which was diluted using LB with chloramphenicol. This experiment was left for 16 hours to give the E. coli time to grow and replicate, see figure 1.