Difference between revisions of "Team:Austin UTexas/Protocols"

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<li> 1.5 g of Citric Acid </li>
 
<li> 1.5 g of Citric Acid </li>
 
<li> <span style="color:red;">16 g of Agar (if making solid media)</span> </li>
 
<li> <span style="color:red;">16 g of Agar (if making solid media)</span> </li>
−
<li> Add distilled water to reach final volume </li>
+
<li> Add distilled water to reach final volume </li> <br> Autoclave the solution
 +
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
 +
 
 +
<span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span>
 +
 
 +
 
 +
<big> '''YPD Media''' </big> <br> '''Introduction''' <br> This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth. <br> '''Materials'''
 +
<ul>
 +
<li> 10 g of Yeast Extract </li>
 +
<li> 20 g of Peptone </li>
 +
<li> <span style="color:red;">16 g of Agar (if making solid media)</span> </li>
 +
<li> Add distilled water to reach final volume </li> <br> Autoclave the solution
 
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
 
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
  
 
<span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span>
 
<span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span>

Revision as of 00:54, 15 July 2016

HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Materials

  • 5 g of Yeast Extract
  • 5 g of Peptone
  • 2.7 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
  • 1.5 g of Citric Acid
  • 16 g of Agar (if making solid media)
  • Add distilled water to reach final volume

    • Autoclave the solution
  • 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)

    • DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS


    YPD Media
    Introduction
    This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
    Materials

    • 10 g of Yeast Extract
    • 20 g of Peptone
    • 16 g of Agar (if making solid media)
    • Add distilled water to reach final volume

      • Autoclave the solution
    • 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
      • DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS