Difference between revisions of "Team:Austin UTexas/Protocols"
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<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | <li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | ||
| − | <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> | + | <li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li> |
<div class="column full_size" > | <div class="column full_size" > | ||
Revision as of 00:59, 15 July 2016
HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Procedure
- 5 g of Yeast Extract
- 5 g of Peptone
- 2.7 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
- 1.5 g of Citric Acid
- 16 g of Agar (if making solid media)
- Add distilled water to reach final volume
- Autoclave the solution
- 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
- DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS
- 10 g of Yeast Extract
- 20 g of Peptone
- 16 g of Agar (if making solid media)
- Add distilled water to reach final volume
- Autoclave the solution
- 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS
YPD Media
Introduction
This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
procedure
