Difference between revisions of "Team:Austin UTexas/Protocols"
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<li> Autoclave the solution </li> | <li> Autoclave the solution </li> | ||
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | <li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | ||
| − | |||
<li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li> | <li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li> | ||
| Line 26: | Line 25: | ||
<li>Autoclave the solution </li> | <li>Autoclave the solution </li> | ||
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | <li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li> | ||
| − | |||
<li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li> | <li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li> | ||
Revision as of 01:00, 15 July 2016
HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Procedure
- 5 g of Yeast Extract
- 5 g of Peptone
- 2.7 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
- 1.5 g of Citric Acid
- 16 g of Agar (if making solid media)
- Add distilled water to reach final volume
- Autoclave the solution
- 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
- DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS
- 10 g of Yeast Extract
- 20 g of Peptone
- 16 g of Agar (if making solid media)
- Add distilled water to reach final volume
- Autoclave the solution
- 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
- DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS
YPD Media
Introduction
This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
procedure
