Difference between revisions of "Team:Austin UTexas/Protocols"

Line 14: Line 14:
 
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
 
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
 
<li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li>  
 
<li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li>  
−
</div>
+
 
  
 
<div class="column full_size" >
 
<div class="column full_size" >
Line 26: Line 26:
 
<li>Autoclave the solution </li>
 
<li>Autoclave the solution </li>
 
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
 
<li> 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved) </li>
−
<li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li>
+
<li> <span style="color:red;">'''DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS'''</span> </li>
−
</div>
+

Revision as of 01:04, 15 July 2016

HS Media
Introduction
This is the recipe to make 1 Liter of HS (High Salt) medium.
Procedure

  • 5 g of Yeast Extract
  • 5 g of Peptone
  • 2.7 g of Sodium Phosphate, dibasic anhydrous (Na2HPO4)
  • 1.5 g of Citric Acid
  • 16 g of Agar (if making solid media)
  • Add distilled water to reach final volume
  • Autoclave the solution
  • 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
  • DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS


    • YPD Media
    Introduction
    This is the recipe to make 1 Liter of YPD medium which is a complete medium for yeast growth.
    procedure

    • 10 g of Yeast Extract
    • 20 g of Peptone
    • 16 g of Agar (if making solid media)
    • Add distilled water to reach final volume
    • Autoclave the solution
    • 50 mL of Sterile 40% (w/v) glucose (added after the other solution is autoclaved)
    • DO NOT ADD GLUCOSE PRIOR TO AUTOCLAVING THE SOLUTION TO AVOID CREATING TOXIC BYPRODUCTS