Project

Our project face the growing problem of plastic pollution and the evolution of antibiotic resistant bacteria. Bacto-Aid is a sustainable concept with bacteria aiding us. We focused on producing a bandage, consisting of recombinant spider silk integrated with antimicrobial peptides, making it preventive towards infections. The plastic, of which we wish to attach the spider silk to, is biodegradable and produced in the lab.

Bacteriocins

Spider silk

poly-β-hydroxybutyrate PHB

In order to realize our project we set out to; 1) test the antimicrobial effects of different bacteriocins on different strains of bacteria, 2) assemble a silk expressing gene-constructs, and 3) optimize the PHB production. Additionally, we tried to create a hybrid silk gene-construct, in which bacteriocins are incorporated between the silk monomers.

• The bacteriocins was found to be more efficient towards S. aureus than traditional antibiotics are. Furthermore we designed our own hybrid bacteriocins and proved a synergistic effect was achieved by doing so.


• We worked hard trying to produce a silk construct we could purify. However, we discovered too late that our unsuccessful attempts probably was due to expired streptavidin beads (which are crucial for our silk assembly method).


• We managed to optimize the production of PHB X-fold compared to previous iGEM teams. The increased production enabled us to synthesize vast amounts of PHB. Some of the produced plastic was used for 3D printing a piece of a jaw to proof the usefulness of our biodegradable plastic.

Scientific reproducibility has been a cornerstone of over project from the start, since we have been using other iGEM projects as basis for the development of our Bacto-Aid. We have likewise focused on reproducibility of our own project in order to assure that the good ideas lives on. Our results will first become interesting the moment reproducibility is assured.

So let’s get down to the details.