Difference between revisions of "Team:Newcastle/Demonstrate"
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<p>Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario. | <p>Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario. | ||
| − | <figure><a="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" data-lightbox="img" data-title="Figure 1: We placed a microfluidic chip containing E. coli transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42 DEGREES before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in E. coli at this temperature."><img src="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" width=100% /></a><figcaption>Figure 1: We placed a microfluidic chip containing E. coli transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42 DEGREES before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in E. coli at this temperature</figcaption></figure></p> | + | <figure><a="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" data-lightbox="img" data-title="Figure 1: We placed a microfluidic chip containing <em>E. coli</em> transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42 DEGREES before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in <em>E. coli</em> at this temperature."><img src="https://static.igem.org/mediawiki/2016/f/f8/T--Newcastle--boardUV.jpg" width=100% /></a><figcaption>Figure 1: We placed a microfluidic chip containing <em>E. coli</em> transformed with our BBa_K1895000 construct onto our magnetic breadboard system and captured this image under UV light, indicating that the fluorescing bacteria can be observed. Note - this is a technical recreation wherein the cells were placed in a shake incubator at 42 DEGREES before being injected in the chip due to technical difficulties. We have previously demonstrated that we can successfully achieve the required temperature change within the chamber to induce sfGFP expression, and that this construct is expressed in E. coli at this temperature</figcaption></figure></p> |
| − | <figure><a="https://static.igem.org/mediawiki/2016/6/6d/T--Newcastle--sixthform.png" data-lightbox="img" data-title="Figure 2: We placed the miniature microbial fuel cell construct containing E. coli transformed with BBa_K1895004 and another microfluidic chip containing 1M NaCl solution onto our breadboard, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery' was as we expected based on our previous results (LINK HERE)"><img src="https://static.igem.org/mediawiki/2016/6/6d/T--Newcastle--sixthform.png" width=100% /></a><figcaption>Figure 2: We placed the miniature microbial fuel cell construct containing E. coli transformed with BBa_K1895004 and another microfluidic chip containing 1M NaCl solution, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery') was as we expected based on our previous results (LINK HERE).</figcaption></figure></p> | + | <figure><a="https://static.igem.org/mediawiki/2016/6/6d/T--Newcastle--sixthform.png" data-lightbox="img" data-title="Figure 2: We placed the miniature microbial fuel cell construct containing <em>E. coli</em> transformed with BBa_K1895004 and another microfluidic chip containing 1M NaCl solution onto our breadboard, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery' was as we expected based on our previous results (LINK HERE)"><img src="https://static.igem.org/mediawiki/2016/6/6d/T--Newcastle--sixthform.png" width=100% /></a><figcaption>Figure 2: We placed the miniature microbial fuel cell construct containing <em>E. coli </em> transformed with BBa_K1895004 and another microfluidic chip containing 1M NaCl solution, connecting them via our hardware connector pieces. We confirmed using a multimeter that the voltage across the receiving chip (being output from the 'battery') was as we expected based on our previous results (LINK HERE).</figcaption></figure></p> |
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Revision as of 23:54, 19 October 2016
Having successfully shown that our genetic constructs worked as expected under lab conditions, we had the final challenge of integrating them into the breadboard hardware that we had designed to illustrate a simulated real-world use-case scenario.
