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This year, our project was based on the heterologous expression of spider silk protein in the microalgae <i> Chlamydomonas reinhardtii</i>. We named it AlgAranha, a combination of the portuguese words for algae and spider. Besides the goal of producing enzybiotics and monomers of spider silk, we aim to achieve an improvement of <i> Chlamydomonas </i> as a synbio chassis.<!--The project started when we looked at the problem of growing antibiotic resistance and started to think in ways to tackle it. We specially focused on injury related infections, for example in the case of burn victms. We devised the creation of an antibiotic patch, combining the spider silk physical properties with antibiotic enzymes (enzybiotics). We intend to express both the spider silk and chimeric enzybiotic proteins with spider silk motifs in <i> Chlamydomonas </i> and polymerize them together to form the product of interest. We hope to accomplish, besides the final goal of patch development, improvement of <i> Chlamydomonas </i> as a synbio chassis and analysis of its capability of producing enzybiotics and monomers of spider silk.--> Moreover, the team is involved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public. </p> | This year, our project was based on the heterologous expression of spider silk protein in the microalgae <i> Chlamydomonas reinhardtii</i>. We named it AlgAranha, a combination of the portuguese words for algae and spider. Besides the goal of producing enzybiotics and monomers of spider silk, we aim to achieve an improvement of <i> Chlamydomonas </i> as a synbio chassis.<!--The project started when we looked at the problem of growing antibiotic resistance and started to think in ways to tackle it. We specially focused on injury related infections, for example in the case of burn victms. We devised the creation of an antibiotic patch, combining the spider silk physical properties with antibiotic enzymes (enzybiotics). We intend to express both the spider silk and chimeric enzybiotic proteins with spider silk motifs in <i> Chlamydomonas </i> and polymerize them together to form the product of interest. We hope to accomplish, besides the final goal of patch development, improvement of <i> Chlamydomonas </i> as a synbio chassis and analysis of its capability of producing enzybiotics and monomers of spider silk.--> Moreover, the team is involved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public. </p> | ||
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Revision as of 21:55, 2 December 2016
AlgAranha Team USP_UNIFESP-Brazil
Unfortunately, we were not able to achieve the end goal of silk production in Chlamydomonas reinhardtii, but we managed to do some nice things (and get a SILVER MEDAL!) Here you can find stuff about:
Our DIY Centrifuge
Our efforts to assemble the spider silk genes at the USER multimerization part of our lab notebook
Transforming Chlamydomonas reinhardtii to produce heterologous proteins, with results and protocols
If you're interested in contacting us, don't hesitate! Whether it's answering questions, just chatting (about SynBio or not, your pick!), making plans for the future or inviting us to go grab a coffee, we would be happy to reply. You can do so at our Facebook page or by emailing us at igemsp2016@gmail.com
We are a multidisciplinary team from São Paulo, Brazil, with students from different majors, as architecture, biology, biomedical sciences, social sciences, and also from the universities USP, UNESP and UNIFESP. The team was originated from the synthetic biology club (SynBio Brasil), which is an independent group that works promoting synbio and open science awareness and education. Since 2012, different club members have organized themselves to take part in iGEM competition.
This year, our project was based on the heterologous expression of spider silk protein in the microalgae Chlamydomonas reinhardtii. We named it AlgAranha, a combination of the portuguese words for algae and spider. Besides the goal of producing enzybiotics and monomers of spider silk, we aim to achieve an improvement of Chlamydomonas as a synbio chassis. Moreover, the team is involved with open hardware developement and promotion and synthetic biology popularization, helping to promote the synthetic biology culture in Brazil, raising awareness and engaging the public.
