Difference between revisions of "Team:Paris Saclay/Notebook/July/27"
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We obtained transformed bacteria for every conditions made yesterday (except for controls). | We obtained transformed bacteria for every conditions made yesterday (except for controls). | ||
| − | File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg | + | [[File:T--Paris_Saclay--160711_characterization_K13-TAA.jpg|400px|thumb|right|Colonies of BL21|pcl_TAA and K1372001]] |
We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm. | We wanted to have glycerol stock of our transformed bacteria so we had to make a pre-culture. Cells were put in 2mL of LB + Streptomycin (50µg/mL) + Chloramphenicol (30µg/mL) medium. They were incubated overnight at 37°C, 180 rpm. | ||
Revision as of 12:34, 27 July 2016
