Difference between revisions of "Team:Paris Saclay/Notebook/July/28"
(→Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009) |
(→Low Fidelity DreamTaqPCR of DH5alpha transformed with pPS16_005 and pPS16_009) |
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| Line 8: | Line 8: | ||
PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. | PCR was performed on 6 clones for DH5a|pPS16_005 and 6 clones for DH5a|pPS16_009. | ||
| − | Thus, the PCR mix was done for 12 tubes following the usual protocol. | + | Thus, the PCR mix was done for 12 tubes following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]]. |
Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. | Each clone was taken off from the petri dish (27/07) soaked in a PCR mix and finally re-plated on a Petri dish (LB+AMP+X-Gal+IPTG. | ||
Revision as of 10:01, 28 July 2016
