Monday 1^st August

Lab work

Visualization

New gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB insertion in puc19

By Léa and Charlène

The insertion of these gblocks was carried out following the usual protocol.

Transformation of new gBlocks and 1.2, 1.2, 3.1 and 4.1 inserted in puc19

By Léa and Mathilde

gBlocks spacer 1, Nm_sgRNA, ATG_linker-FKBP, St_sgRNA, Detection, ATG_linker-RFB,1.2, 1.2, 3.1 and 4.1 inserted in puc19 were transformed following the usual protocol. For each plasmid we streaked 50µL and 150µL of bacteria on LB + X-Gal + IPTG Petri dishes.

gBlock 3.1 insertion in puc19

By Caroline

The insertion was carried out following the usual protocol.

DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007 streak to recover colonies

By Caroline

Checked DH5alp transformed with pPS16_003, pPS16_004, pPS16_006 and pPS16_007 were streaked on Pteri dishes containing LB + Amp (50µg/mL) + IPTG (0.1 µL/mL) + Xgal (0.25µL/mL) from glycerol cultures.