Difference between revisions of "Team:Paris Saclay/Notebook/August/2"
(→Monday 1st August) |
(→DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015) |
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==Lab work== | ==Lab work== | ||
===Visualization=== | ===Visualization=== | ||
| − | ==== | + | ==== DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015 ==== |
''By Charlène, Mathilde, Laetitia, Caroline and Léa'' | ''By Charlène, Mathilde, Laetitia, Caroline and Léa'' | ||
A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] and using the universal puc19 [[Team:Paris_Saclay/Experiments#primers|primers]]. 6 clones for each transformations were tested. | A Low fidelity PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#Polymerase_chain_reaction|protocol]] and using the universal puc19 [[Team:Paris_Saclay/Experiments#primers|primers]]. 6 clones for each transformations were tested. | ||
| + | |||
| + | Each clone was re-plated and put at 37°C. | ||
| + | |||
| + | Each clone was also cultured in 3mL of LB and Ampicilin. | ||
==== Sequencing of the gBlock 4.2 ==== | ==== Sequencing of the gBlock 4.2 ==== | ||
Revision as of 11:35, 2 August 2016
