Difference between revisions of "Team:Paris Saclay/Notebook/August/2"

(DreamTap PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015)
(DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015)
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Each clone was re-plated and put at 37°C.
 
Each clone was re-plated and put at 37°C.
 
+
We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm).
Each clone was also cultured in 3mL of LB and Ampicilin.
+
  
 
==== Sequencing of the gBlock 4.2 ====
 
==== Sequencing of the gBlock 4.2 ====

Revision as of 11:37, 2 August 2016

Tuesday 2st August

Lab work

Visualization

DreamTaq PCR on DH5alp|pPS16_001, DH5alp|pPS16_002, DH5alp|pPS16_005, DH5alp|pPS16_009, DH5alp|pPS16_0010, DH5alp|pPS16_011, DH5alp|pPS16_012, DH5alp|pPS16_013, DH5alp|pPS16_014, DH5alp|pPS16_015

By Charlène, Mathilde, Laetitia, Caroline and Léa

A Low fidelity PCR was carried out following the usual protocol and using the universal puc19 primers. 6 clones for each transformations were tested.

Each clone was re-plated and put at 37°C. We also did a liquid culture for each clone in 3mL of LB and Ampicilin(37°C and 180 rpm).

Sequencing of the gBlock 4.2

By Caroline

The PCR products obtain with Q5 on pPS16_008 were send to sequencing.