Difference between revisions of "Team:Paris Saclay/Notebook/August/4"

(DNA Extraction of plasmids containing g blocks 1.1, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP)
(Visualization)
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Extraction was done on:
 
Extraction was done on:
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==== Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP  ====
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''By Naiane, Mathilde and Laetitia''
  
 
+
Stocks of glycerol were made with:
 +
*700 uL of culture
 +
*300 uL of glycerol (60%)
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 13:25, 4 August 2016

Thursday 4st August

Lab work

Visualization

DNA Extraction of plasmids containing g blocks 1.1, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP

By Caroline, Naiane, Mathilde and Laetitia


Extraction was done on:


Puc 19 containing Clone
1.1 2, 9
1.2 4, 7, 11
GFP 7
3.1 4
NM 7, 11
dCas9_TD 1
dCas9_SP 1


The extraction was performed using the kit. We followed this protocol.


Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP

By Naiane, Mathilde and Laetitia

Stocks of glycerol were made with:

  • 700 uL of culture
  • 300 uL of glycerol (60%)