Difference between revisions of "Team:Paris Saclay/Notebook/August/4"

(DNA Extraction of plasmids containing g blocks 1.1, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP)
(Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP)
Line 40: Line 40:
 
The extraction was performed using the kit. We followed this [[Team:Paris_Saclay/Experiments#DNA_extraction_using_the_Invitrogen_ChargeSwitch.C2.AE-Pro_Plasmid_Miniprep_Kit|protocol]].
 
The extraction was performed using the kit. We followed this [[Team:Paris_Saclay/Experiments#DNA_extraction_using_the_Invitrogen_ChargeSwitch.C2.AE-Pro_Plasmid_Miniprep_Kit|protocol]].
  
==== Stock of glycerol pPS_, 1.2,3.1, GFP,NM, dCas9-TD, dCas9-SP  ====
+
==== Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009  ====
 
''By Naiane, Mathilde and Laetitia''
 
''By Naiane, Mathilde and Laetitia''
  

Revision as of 13:48, 4 August 2016

Thursday 4st August

Lab work

Visualization

DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN-

By Caroline, Naiane, Mathilde and Laetitia


Extraction was done on:


Puc 19 containing Clone
pPS16_001 2, 9
pPS16_002 4, 7, 11
pPS16_009 7
pPS16_003 4
DS-NMcasN- 7, 11
DS-TDcasN- 1
DS-SPcasN- 1


The extraction was performed using the kit. We followed this protocol.

Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009

By Naiane, Mathilde and Laetitia

Stocks of glycerol were made with:

  • 700 uL of culture
  • 300 uL of glycerol (60%)