Difference between revisions of "Team:Paris Saclay/Notebook/August/4"

Revision as of 14:17, 4 August 2016

Contents

1 Thursday 4^st August
- 1.1 Lab work
  - 1.1.1 Visualization
    - 1.1.1.1 DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN-
    - 1.1.1.2 Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009 and DS-NMcasN-

Thursday 4^st August

Lab work

Visualization

DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN-

By Caroline, Naiane, Mathilde and Laetitia

Extraction was done on:

Puc 19 containing	Clone
pPS16_001	2, 9
pPS16_002	4, 7, 11
pPS16_009	7
pPS16_003	4
DS-NMcasN-	7, 11
DS-TDcasN-	1
DS-SPcasN-	1

The extraction was performed using the kit. We followed this protocol.

Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009 and DS-NMcasN-

By Naiane, Mathilde and Laetitia

Stocks of glycerol were made with:

700 µL of culture
300 µL of glycerol (60%)