Friday 5^st August

Lab work

Visualization

DNA Extraction of DS-TDcasN- and DS-SPcasN-

By Laetitia

The extraction was performed using the kit. We followed this protocol.

The initial culture was at 15 mL so we increased (*4) the volumes until the precipitation.

Hence we used:

-1 ml of resuspension buffer

-1 ml lysis buffer

-1 ml precipitation buffer

The colum was used several times in order to recover the maximum of DNA

DNA Extraction of DS-NMcasN- and pPS_001

By Mathilde

The extraction was performed on clone 11 for NM and clone 9 for 1.1 using the kit. We followed this protocol.

Q5 PCR on DH5alp|pPS16_003, DH5alp|pPS16_004, DH5alp|pPS16_006 and DH5alp|pPS16_007

By Caroline

The PCR was carried out following the usual protocol adapted to 50µL and with a TM at 70°C. The specific primers for each parts were using.