Difference between revisions of "Team:Paris Saclay/Notebook/July/29"
(→High fidelity PCR on bacteria transformed with pPS16_001, pPS16_002 and pPS16_005) |
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{{Team:Paris_Saclay/notebook_header}} | {{Team:Paris_Saclay/notebook_header}} | ||
= Friday 29<sup>th</sup> July= | = Friday 29<sup>th</sup> July= | ||
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===Visualization=== | ===Visualization=== | ||
====gBlock 1.1, 1.2 and GFP1-9 insertion in puc19==== | ====gBlock 1.1, 1.2 and GFP1-9 insertion in puc19==== | ||
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''By Alice and Mathilde '' | ''By Alice and Mathilde '' | ||
| − | A PCR with Q5® High-Fidelity 2X Master Mix was performed on clones selected after screening PCR made on July 28, following [[Team:Paris_Saclay/Experiments#Q5PCR|this protocol]]. We choose clones which had the good size of insert. We selected the clone 2 for DH5α|pPS16_001; clones 4,7 and 8 for DH5α|pPS16_002 and clones 1 and 4 DH5α|pPS16_005. [[Team:Paris_Saclay/Experiments#primers|1151_pheoR and 1152_pheoF]] primers were used. Annealing temperature was 66°C | + | A PCR with Q5® High-Fidelity 2X Master Mix was performed on clones selected after screening PCR made on July 28, following [[Team:Paris_Saclay/Experiments#Q5PCR|this protocol]]. We choose clones which had the good size of insert. We selected the clone 2 for DH5α|pPS16_001; clones 4,7 and 8 for DH5α|pPS16_002 and clones 1 and 4 DH5α|pPS16_005. [[Team:Paris_Saclay/Experiments#primers|1151_pheoR and 1152_pheoF]] primers were used. Annealing temperature was 66°C. After amplification, 1 µL of loading dye was added to 5µL of each PCR products. Then 5 µL of this mix were put on gel. PCR products were migrated 30min at 100V. |
PCR products expected were : | PCR products expected were : | ||
Latest revision as of 16:13, 9 October 2016
