Difference between revisions of "Team:Paris Saclay/Notebook/August/12"
(→PCR of pPS16_009) |
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Primers: IPS83 ans IPS84 | Primers: IPS83 ans IPS84 | ||
| + | ====Extraction of puc19==== | ||
| + | ''By Charlène'' | ||
| + | |||
| + | Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]]. | ||
| + | They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water. | ||
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 12:52, 12 August 2016
