Difference between revisions of "Team:Paris Saclay/Notebook/August/12"
(→Extraction of puc19) |
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Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]]. | Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]]. | ||
| − | They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water. | + | They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C. |
{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 12:53, 12 August 2016
