(→Extraction of puc19) |
(→Lab work) |
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They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C. | They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C. | ||
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+ | ====PCR==== | ||
+ | ''By Naiane, Mahnaz and Terrence'' | ||
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+ | The PCR was carried out following the usual [[Team:Paris_Saclay/Experiments#taqPCR|protocol]] | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 15:25, 12 August 2016