Difference between revisions of "Team:Alverno CA/InterLab"

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</head>
  
 +
<body>
 +
<h1><center>Alverno iGEM Interlab Study</center></h1>
 +
<h1><center>Quantifying different sources of variation in gene expression using
 +
fluorescent transformed E. coli bacteria</center></h1>
 +
<center><img src="https://s-media-cache-ak0.pinimg.com/originals/86/08/18/860818cfc65e5ff04725bb0f0c05a8af.png" alt="Alverno iGEM Logo" style="width:300px;"></center>
  
 +
<p><h3>Summary:</h3> </p>
 +
<p>Leverage agile frameworks to provide a robust synopsis for high level overviews. Iterative approaches to corporate strategy foster collaborative thinking to further the overall value proposition. Organically grow the holistic world view of disruptive innovation via workplace diversity and empowerment.</p>
  
 +
<p><h3>Project Description:</h3></p>
 +
<p>We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with an OD600 reference point and FITC standard curve.<p>
  
<div class="menu_wrapper" >
+
<p><h3>Protocol:</h3> </p>
 
+
<p><a href="https://static.igem.org/mediawiki/2016/c/c5/InterLab_iGEM2016_Plate_Reader_Protocol_Updated_July.pdf">Plate Reader Protocol</a></p>  
 
+
<p><a href="https://static.igem.org/mediawiki/parts/6/67/IGEM_Registry_-_Transformation_Protocol.pdf">Transformation Protocol</a></p>  
 
+
 
+
<div class="collapsable_menu_control"> MENU ▤ </div>
+
 
+
<ul id="accordion" class="accordion">
+
 
+
<li class="menu_item"> <a href="https://2016.igem.org/Team:Alverno_CA">HOME </a> </li>
+
 
+
 
+
<li class="menu_item"> <div class="icon plus"></div> TEAM
+
<ul class="submenu">
+
<li> <a href=" https://2016.igem.org/Team:Alverno_CA/Team"> Team  </a> </li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Collaborations">★  Collaborations </a> </li>
+
                    </ul>
+
                </li>
+
 
+
<li class="menu_item"> <div class="icon plus"></div> PROJECT 
+
<ul class="submenu">
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Description"> ★  Description </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Design"> ★ Design </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Experiments"> Experiments </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/InterLab"> ★  Interlab Study </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Proof"> ★ Proof of Concept </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Demonstrate"> ★ Demonstrate </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Results"> Results </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Notebook"> Notebook </a></li>
+
                    </ul>
+
                </li>
+
 
+
<li class="menu_item"> <div class="icon plus"></div> PARTS 
+
<ul class="submenu">
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Parts">Parts </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Basic_Part"> ★ Basic Parts </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Composite_Part"> ★ Composite Parts </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Part_Collection"> ★ Part Collection </a></li>
+
                    </ul>
+
                </li>
+
 
+
 
+
<li class="menu_item"> <a href="https://2016.igem.org/Team:Alverno_CA/Safety"> SAFETY </a> </li>
+
 
+
 
+
<li class="menu_item"> <a href="https://2016.igem.org/Team:Alverno_CA/Attributions">★  ATTRIBUTIONS </a></li>
+
 
+
 
+
<li class="menu_item"> <div class="icon plus"></div> HUMAN PRACTICES
+
<ul class="submenu">
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Human_Practices"> Human Practices </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/HP/Silver">★ Silver </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/HP/Gold">★ Gold </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Integrated_Practices"> ★ Integrated Practices </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Engagement">★ Engagement </a></li>
+
                    </ul>
+
                </li>
+
 
+
 
+
<li class="menu_item"> <div class="icon plus"></div> AWARDS
+
<ul class="submenu">
+
<li><a href="https://2016.igem.org/Team:Alverno_CA/Entrepreneurship"> ★ Entrepreneurship </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Hardware"> ★ Hardware </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Software">★ Software </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Measurement">★  Measurement </a></li>
+
<li> <a href="https://2016.igem.org/Team:Alverno_CA/Model">★ Model </a></li>
+
 
+
                    </ul>
+
                </li>
+
</ul>
+
 
+
</div>
+
 
+
 
+
 
+
 
+
 
+
<div class="content_wrapper">
+
 
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+
 
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<h1 id="team_name">  </h1>
+
<h4 id="page_name">  </h4>
+
 
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+
<p><h3>Results:</h3></p>
 +
<center><img src="http://placehold.it/350" alt="Placeholder Image" style="width:300px;"></center>
  
 +
</body>
 
</html>
 
</html>

Revision as of 19:56, 12 August 2016

Alverno iGEM 2016

Alverno iGEM Interlab Study

Quantifying different sources of variation in gene expression using fluorescent transformed E. coli bacteria

Alverno iGEM Logo

Summary:

Leverage agile frameworks to provide a robust synopsis for high level overviews. Iterative approaches to corporate strategy foster collaborative thinking to further the overall value proposition. Organically grow the holistic world view of disruptive innovation via workplace diversity and empowerment.

Project Description:

We tested 3 different fluorescent protein expression plasmids, J23101, J23106, and J23117. We transformed the parts with iGEM's transformation protocol. Our bacteria was cultured on a LB agar plate and cultured in LB broth. To measure the differences in fluorescence caused by each plasmid, we used a plate reader with an OD600 reference point and FITC standard curve.

Protocol:

Plate Reader Protocol

Transformation Protocol

Results:

Placeholder Image