Difference between revisions of "Team:Paris Saclay/Notebook/August/12"
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Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]]. | Clones 1 and 2 of puc19 were extracted with the [[Team:Paris_Saclay/Experiments#InvitrogenPlasmidExtraction|Plasmid MiniPrep kit]]. | ||
| − | They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C. | + | They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C. |
| − | + | ||
| − | + | ||
====Dreamtaq PCR on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2==== | ====Dreamtaq PCR on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2==== | ||
Revision as of 09:33, 17 August 2016
