Revision as of 13:54, 17 August 2016

Friday 12^th August

By Léa

A Dreamtaq PCR was performed on pPS16_009 using the usual protocol. Primers: IPS83 ans IPS84

Electrophoresis of PCR products, using primers IPS 83 and IPS 84 on pPS16_016.

By Charlène

Clones 1 and 2 of puc19 were extracted with the Plasmid MiniPrep kit.

They were fast digested with EcoRI : 3µL of plasmid + 1µL of FD buffer + 0.5µL of FD EcoRI + 5.5µL of water, 15 minutes at 37°C.

By Naiane, Mahnaz and Terrence

A Dreamtaq PCR was performed on puc19, detection, FRB, FKBP, SgRNAst, SgRNAnm and gBlock 1.2 using the usual protocol with these volumes :

We made 6 clones of each colonie except for Puc19 where we made 1 negative control.

Result of the PCR :

1.2 and FRB

Detection and ST

NM and FKBP

pub19 and FKBP

@@ Line 48: / Line 48: @@
 |}
 We made 6 clones of each colonie except for Puc19 where we made 1 negative control.
+Result of the PCR :
+[[File:T--Paris Saclay--1-2-FRB 15 08.jpeg|400px|thumb|right|1.2 and FRB]]
+[[File:T--Paris_Saclay--détection-ST_15_08.JPG|400px|thumb|right|Detection and ST]]
+[[File:T--Paris_Saclay--NM-FKBP_15_08.JPG|400px|thumb|right|NM and FKBP]]
+[[File:T--Paris_Saclay--puc19-FKBP_15_08.JPG|400px|thumb|right|pub19 and FKBP]]