|
|
| Line 29: |
Line 29: |
| | 3 clones from each transformation condition were grown in 500µL of LB, streptomycin (50µg/mL) and chloramphenicol (30µg/mL). | | 3 clones from each transformation condition were grown in 500µL of LB, streptomycin (50µg/mL) and chloramphenicol (30µg/mL). |
| | Then each solution was split into 3x150µL and 350µL of mix of LB + streptomycin (50µg/mL) + chloramphenicol (30µg/mL) were added to each culture. | | Then each solution was split into 3x150µL and 350µL of mix of LB + streptomycin (50µg/mL) + chloramphenicol (30µg/mL) were added to each culture. |
| − | Salicylate (main solution at 10mM) was added to obtain the following concentrations : | + | Salicylate (main solution at 10mM) was added to obtain the following concentrations : 0, 0.03mM and 0.1mM. |
| − | | + | |
| − | {| class="wikitable"
| + | |
| − | |-
| + | |
| − | !Plasmid(s)
| + | |
| − | |colspan="9"|K1372001
| + | |
| − | | + | |
| − | |colspan="9"|K1372001 + pcl_TAA
| + | |
| − | |colspan="9"|K1372001 + pcl_TAG
| + | |
| − | |colspan="9"|K1372001 + pcl_Tq
| + | |
| − | |-
| + | |
| − | !Clone
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |1
| + | |
| − | |2
| + | |
| − | |3
| + | |
| − | |-
| + | |
| − | !Salicylate concentration
| + | |
| − | |colspan="3"|0
| + | |
| − | |colspan="3"|30µM
| + | |
| − | |colspan="3"|1mM
| + | |
| − | |colspan="3"|0
| + | |
| − | |colspan="3"|30µM
| + | |
| − | |colspan="3"|1mM
| + | |
| − | |colspan="3"|0
| + | |
| − | |colspan="3"|30µM
| + | |
| − | |colspan="3"|1mM
| + | |
| − | |colspan="3"|0
| + | |
| − | |colspan="3"|30µM
| + | |
| − | |colspan="3"|1mM
| + | |
| − | |}
| + | |
| − | | + | |
| − | {{Team:Paris_Saclay/notebook_footer}}
| + | |
| | | | |
| | {{Team:Paris_Saclay/notebook_footer}} | | {{Team:Paris_Saclay/notebook_footer}} |
Revision as of 15:18, 17 August 2016
