Thursday 4^th August

Lab work

Visualization

DNA Extraction of pPS16_001, pPS16_002, pPS16_003, pPS16_009,DS-NMcasN-, DS-TDcasN-, DS-SPcasN-

By Caroline, Naiane, Mathilde and Laetitia

Extraction was done on:

The extraction was performed using the ChargeSwitch-Pro Plasmid MiniPrep kit from Invitrogen. We followed this protocol.

Stock of glycerol for pPS16_001, pPS16_002, pPS16_003, pPS16_009 and DS-NMcasN-

By Naiane, Mathilde and Laetitia

Stocks of glycerol were made with:

• 700 µL of culture
• 300 µL of glycerol (60%)

PCR Clean-up with the NucleoSpin kit

By Caroline

The purification was carried out on DH5alp|pPS16_003, DH5alp|pPS16_004 and DH5alp|pPS16_007 following the usual protocol. But, I forgot to dilute the sample with the Buffer NT3 with 100mL ethanol befor I began the purification and so no DNA was present on the 0.8% agarose gel.

pPS16_004, pPS16_006 and pPS16_007

By Caroline

Q5 PCR was carried out following the usual protocol.

Result of the PCR