Difference between revisions of "Team:Paris Saclay/Notebook/August/19"
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A previous step of denaturation (95°C, 5min) was performed for colony PCR. | A previous step of denaturation (95°C, 5min) was performed for colony PCR. | ||
| − | [[File:T--Paris Saclay--190826 visualization PCR1.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products 1.2 gBlock. | + | [[File:T--Paris Saclay--190826 visualization PCR1.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products 1.2 gBlock.]] |
Only the sample from the clone number 14 seems to have an appropriate size (expected at 1073 bp). This clone will be grown and the plasmid will be extracted to be sequenced. | Only the sample from the clone number 14 seems to have an appropriate size (expected at 1073 bp). This clone will be grown and the plasmid will be extracted to be sequenced. | ||
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| − | [[File:T--Paris_Saclay--190826_visualization_PCR2.JPG|500px|thumb|right|Gel electrophoresis of PCR products FRB, FKBP and sgRNA Nm gBlocks. | + | [[File:T--Paris_Saclay--190826_visualization_PCR2.JPG|500px|thumb|right|Gel electrophoresis of PCR products FRB, FKBP and sgRNA Nm gBlocks.]] |
For FRB (492bp), plasmid extractions from clones 5 and 2 seem to have an appropriate size and will be send for sequencing. | For FRB (492bp), plasmid extractions from clones 5 and 2 seem to have an appropriate size and will be send for sequencing. | ||
| − | For FKBP (537bp), the 3 plasmids extraction will be sent. For 1.2 (1073bp), only plasmid extraction from clone 11 will be sent, and no plasmid extraction will be sent for sgRNA Nm (480bp). | + | For FKBP (537bp), the 3 plasmids extraction will be sent. For 1.2 (1073bp), only plasmid extraction from clone 11 will be sent, and no plasmid extraction will be sent for sgRNA Nm (480bp). |
| − | [[File:T--Paris Saclay--190826 visualization PCR3.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products, gBlock sg RNA Nm. | + | [[File:T--Paris Saclay--190826 visualization PCR3.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products, gBlock sg RNA Nm.]] |
No significative stripe is observed. there is no clone carrying the plasmid of interrest. | No significative stripe is observed. there is no clone carrying the plasmid of interrest. | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 11:33, 22 August 2016
