Difference between revisions of "Team:Paris Saclay/Notebook/August/19"
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A previous step of denaturation (95°C, 5min) was performed for colony PCR. | A previous step of denaturation (95°C, 5min) was performed for colony PCR. | ||
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For 1.2 gBlock, only the sample from the clone number 14 seems to have an appropriate size (expected at 1073 bp). This clone will be grown and the plasmid will be extracted to be sequenced. | For 1.2 gBlock, only the sample from the clone number 14 seems to have an appropriate size (expected at 1073 bp). This clone will be grown and the plasmid will be extracted to be sequenced. | ||
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For FRB (492bp), plasmid extractions from clones 5 and 2 seem to have an appropriate size and will be send for sequencing. | For FRB (492bp), plasmid extractions from clones 5 and 2 seem to have an appropriate size and will be send for sequencing. | ||
For FKBP (537bp), the 3 plasmids extraction will be sent. For 1.2 (1073bp), only plasmid extraction from clone 11 will be sent, and no plasmid extraction will be sent for sgRNA Nm (480bp). | For FKBP (537bp), the 3 plasmids extraction will be sent. For 1.2 (1073bp), only plasmid extraction from clone 11 will be sent, and no plasmid extraction will be sent for sgRNA Nm (480bp). | ||
| + | No significative stripe is observed for sgRNA Nm gBlock. there is no clone carrying the plasmid of interrest. | ||
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| + | [[File:T--Paris Saclay--190826 visualization PCR1.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products 1.2 gBlock.]] | ||
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| + | [[File:T--Paris_Saclay--190826_visualization_PCR2.JPG|500px|thumb|right|Gel electrophoresis of PCR products FRB, FKBP and sgRNA Nm gBlocks.]] | ||
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[[File:T--Paris Saclay--190826 visualization PCR3.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products, gBlock sg RNA Nm.]] | [[File:T--Paris Saclay--190826 visualization PCR3.jpeg|500px|thumb|right|Gel electrophoresis of colony PCR products, gBlock sg RNA Nm.]] | ||
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{{Team:Paris_Saclay/notebook_footer}} | {{Team:Paris_Saclay/notebook_footer}} | ||
Revision as of 11:36, 22 August 2016
