====Gibson assembly product transformation in DH5a cells====
====Gibson assembly product transformation in DH5a cells====
−
''by Léa''
+
''By Léa and Terrence''
−
+
−
Gibson assembly product (fragments 3 and 4 ligation) and control were transformed into DH5a cells, using 2 µL of DNA, and following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
+
−
+
−
====pPS16_016 ligation product transformation in DH5a====
+
−
''By Léa''
+
−
+
−
pPS16_016 ligation product of the 10/08/2016 was transformed into DH5a cells, using 2 µL of DNA, and following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
+
−
Transformation products were plated on LB Agar chloramphenicol Petri dishes and incubated at 37°C ON.
+
−
+
−
=== Interlab Study===
+
−
====Transformation into DH5a cells====
+
−
''By Léa''
+
−
+
−
Devices 2 and 3, cnegative control and positive control was transformed into DH5a cells, following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
+
−
Transformation products were plated on LB Agar chloramphenicol Petri dishes and incubated at 37°C ON.
+
−
+
−
====Device 1 culture====
+
−
''By Léa''
+
−
+
−
Device 1 transformed DH5a glycerol stock was plated on LB Agar chloramphenicol Petri Dishes and incubated at 37°C ON.
+
−
+
====Gibson on fragment 3 and fragment 4====
====Gibson on fragment 3 and fragment 4====
Line 164:
Line 143:
We made 2 tubes composed of this upper mix.
We made 2 tubes composed of this upper mix.
In the control one, we added 10 µL of water, whereas in the "Gibson" one, we added 10µL of Gibson mix.
In the control one, we added 10 µL of water, whereas in the "Gibson" one, we added 10µL of Gibson mix.
+
+
+
Gibson assembly product (fragments 3 and 4 ligation) and control were transformed into DH5a cells, using 2 µL of DNA, and following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
+
+
====pPS16_016 ligation product transformation in DH5a====
+
''By Léa''
+
+
pPS16_016 ligation product of the 10/08/2016 was transformed into DH5a cells, using 2 µL of DNA, and following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
+
Transformation products were plated on LB Agar chloramphenicol Petri dishes and incubated at 37°C ON.
+
+
=== Interlab Study===
+
+
====Transformation into DH5a cells====
+
''By Léa''
+
+
Devices 2 and 3, cnegative control and positive control was transformed into DH5a cells, following the usual [[Team:Paris_Saclay/Experiments#heat-shocktransformation|protocol]].
+
Transformation products were plated on LB Agar chloramphenicol Petri dishes and incubated at 37°C ON.
+
+
====Device 1 culture====
+
''By Léa''
+
+
Device 1 transformed DH5a glycerol stock was plated on LB Agar chloramphenicol Petri Dishes and incubated at 37°C ON.
