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<meta charset="utf-8"> | <meta charset="utf-8"> | ||
<meta name="viewport" content="width=device-width, initial-scale=1"> | <meta name="viewport" content="width=device-width, initial-scale=1"> | ||
− | + | <style title="jquery-ui.css"> | |
− | <script | + | /*! jQuery UI - v1.12.0 - 2016-07-08 |
− | + | * http://jqueryui.com | |
− | + | * Includes: core.css, accordion.css, autocomplete.css, menu.css, button.css, controlgroup.css, checkboxradio.css, datepicker.css, dialog.css, draggable.css, resizable.css, progressbar.css, selectable.css, selectmenu.css, slider.css, sortable.css, spinner.css, tabs.css, tooltip.css, theme.css | |
+ | * To view and modify this theme, visit http://jqueryui.com/themeroller/?ffDefault=Lucida%20Grande%2CLucida%20Sans%2CArial%2Csans-serif&fwDefault=bold&fsDefault=1.1em&cornerRadius=6px&bgColorHeader=deedf7&bgTextureHeader=highlight_soft&bgImgOpacityHeader=100&borderColorHeader=aed0ea&fcHeader=222222&iconColorHeader=72a7cf&bgColorContent=f2f5f7&bgTextureContent=highlight_hard&bgImgOpacityContent=100&borderColorContent=dddddd&fcContent=362b36&iconColorContent=72a7cf&bgColorDefault=d7ebf9&bgTextureDefault=glass&bgImgOpacityDefault=80&borderColorDefault=aed0ea&fcDefault=2779aa&iconColorDefault=3d80b3&bgColorHover=e4f1fb&bgTextureHover=glass&bgImgOpacityHover=100&borderColorHover=74b2e2&fcHover=0070a3&iconColorHover=2694e8&bgColorActive=3baae3&bgTextureActive=glass&bgImgOpacityActive=50&borderColorActive=2694e8&fcActive=ffffff&iconColorActive=ffffff&bgColorHighlight=ffef8f&bgTextureHighlight=highlight_soft&bgImgOpacityHighlight=25&borderColorHighlight=f9dd34&fcHighlight=363636&iconColorHighlight=2e83ff&bgColorError=cd0a0a&bgTextureError=flat&bgImgOpacityError=15&borderColorError=cd0a0a&fcError=ffffff&iconColorError=ffffff&bgColorOverlay=eeeeee&bgTextureOverlay=diagonals_thick&bgImgOpacityOverlay=90&opacityOverlay=80&bgColorShadow=000000&bgTextureShadow=highlight_hard&bgImgOpacityShadow=70&opacityShadow=30&thicknessShadow=7px&offsetTopShadow=-7px&offsetLeftShadow=-7px&cornerRadiusShadow=8px | ||
+ | * Copyright jQuery Foundation and other contributors; Licensed MIT */ | ||
+ | |||
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+ | * Copyright jQuery Foundation and other contributors; Licensed MIT */ | ||
+ | |||
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i=t.extend({of:this.active},this.options.position);clearTimeout(this.timer),this.element.find(".ui-menu").not(e.parents(".ui-menu")).hide().attr("aria-hidden","true"),e.show().removeAttr("aria-hidden").attr("aria-expanded","true").position(i)},collapseAll:function(e,i){clearTimeout(this.timer),this.timer=this._delay(function(){var s=i?this.element:t(e&&e.target).closest(this.element.find(".ui-menu"));s.length||(s=this.element),this._close(s),this.blur(e),this._removeClass(s.find(".ui-state-active"),null,"ui-state-active"),this.activeMenu=s},this.delay)},_close:function(t){t||(t=this.active?this.active.parent():this.element),t.find(".ui-menu").hide().attr("aria-hidden","true").attr("aria-expanded","false")},_closeOnDocumentClick:function(e){return!t(e.target).closest(".ui-menu").length},_isDivider:function(t){return!/[^\-\u2014\u2013\s]/.test(t.text())},collapse:function(t){var e=this.active&&this.active.parent().closest(".ui-menu-item",this.element);e&&e.length&&(this._close(),this.focus(t,e))},expand:function(t){var e=this.active&&this.active.children(".ui-menu ").find(this.options.items).first();e&&e.length&&(this._open(e.parent()),this._delay(function(){this.focus(t,e)}))},next:function(t){this._move("next","first",t)},previous:function(t){this._move("prev","last",t)},isFirstItem:function(){return this.active&&!this.active.prevAll(".ui-menu-item").length},isLastItem:function(){return this.active&&!this.active.nextAll(".ui-menu-item").length},_move:function(t,e,i){var s;this.active&&(s="first"===t||"last"===t?this.active["first"===t?"prevAll":"nextAll"](".ui-menu-item").eq(-1):this.active[t+"All"](".ui-menu-item").eq(0)),s&&s.length&&this.active||(s=this.activeMenu.find(this.options.items)[e]()),this.focus(i,s)},nextPage:function(e){var i,s,n;return this.active?(this.isLastItem()||(this._hasScroll()?(s=this.active.offset().top,n=this.element.height(),this.active.nextAll(".ui-menu-item").each(function(){return i=t(this),0>i.offset().top-s-n}),this.focus(e,i)):this.focus(e,this.activeMenu.find(this.options.items)[this.active?"last":"first"]())),void 0):(this.next(e),void 0)},previousPage:function(e){var i,s,n;return this.active?(this.isFirstItem()||(this._hasScroll()?(s=this.active.offset().top,n=this.element.height(),this.active.prevAll(".ui-menu-item").each(function(){return i=t(this),i.offset().top-s+n>0}),this.focus(e,i)):this.focus(e,this.activeMenu.find(this.options.items).first())),void 0):(this.next(e),void 0)},_hasScroll:function(){return this.element.outerHeight()<this.element.prop("scrollHeight")},select:function(e){this.active=this.active||t(e.target).closest(".ui-menu-item");var i={item:this.active};this.active.has(".ui-menu").length||this.collapseAll(e,!0),this._trigger("select",e,i)},_filterMenuItems:function(e){var i=e.replace(/[\-\[\]{}()*+?.,\\\^$|#\s]/g,"\\$&"),s=RegExp("^"+i,"i");return this.activeMenu.find(this.options.items).filter(".ui-menu-item").filter(function(){return s.test(t.trim(t(this).children(".ui-menu-item-wrapper").text()))})}}),t.widget("ui.autocomplete",{version:"1.12.0",defaultElement:"<input>",options:{appendTo:null,autoFocus:!1,delay:300,minLength:1,position:{my:"left top",at:"left bottom",collision:"none"},source:null,change:null,close:null,focus:null,open:null,response:null,search:null,select:null},requestIndex:0,pending:0,_create:function(){var e,i,s,n=this.element[0].nodeName.toLowerCase(),o="textarea"===n,a="input"===n;this.isMultiLine=o||!a&&this._isContentEditable(this.element),this.valueMethod=this.element[o||a?"val":"text"],this.isNewMenu=!0,this._addClass("ui-autocomplete-input"),this.element.attr("autocomplete","off"),this._on(this.element,{keydown:function(n){if(this.element.prop("readOnly"))return e=!0,s=!0,i=!0,void 0; | ||
+ | e=!1,s=!1,i=!1;var o=t.ui.keyCode;switch(n.keyCode){case o.PAGE_UP:e=!0,this._move("previousPage",n);break;case o.PAGE_DOWN:e=!0,this._move("nextPage",n);break;case o.UP:e=!0,this._keyEvent("previous",n);break;case o.DOWN:e=!0,this._keyEvent("next",n);break;case o.ENTER:this.menu.active&&(e=!0,n.preventDefault(),this.menu.select(n));break;case o.TAB:this.menu.active&&this.menu.select(n);break;case o.ESCAPE:this.menu.element.is(":visible")&&(this.isMultiLine||this._value(this.term),this.close(n),n.preventDefault());break;default:i=!0,this._searchTimeout(n)}},keypress:function(s){if(e)return e=!1,(!this.isMultiLine||this.menu.element.is(":visible"))&&s.preventDefault(),void 0;if(!i){var n=t.ui.keyCode;switch(s.keyCode){case n.PAGE_UP:this._move("previousPage",s);break;case n.PAGE_DOWN:this._move("nextPage",s);break;case n.UP:this._keyEvent("previous",s);break;case n.DOWN:this._keyEvent("next",s)}}},input:function(t){return s?(s=!1,t.preventDefault(),void 0):(this._searchTimeout(t),void 0)},focus:function(){this.selectedItem=null,this.previous=this._value()},blur:function(t){return this.cancelBlur?(delete this.cancelBlur,void 0):(clearTimeout(this.searching),this.close(t),this._change(t),void 0)}}),this._initSource(),this.menu=t("<ul>").appendTo(this._appendTo()).menu({role:null}).hide().menu("instance"),this._addClass(this.menu.element,"ui-autocomplete","ui-front"),this._on(this.menu.element,{mousedown:function(e){e.preventDefault(),this.cancelBlur=!0,this._delay(function(){delete this.cancelBlur,this.element[0]!==t.ui.safeActiveElement(this.document[0])&&this.element.trigger("focus")})},menufocus:function(e,i){var s,n;return this.isNewMenu&&(this.isNewMenu=!1,e.originalEvent&&/^mouse/.test(e.originalEvent.type))?(this.menu.blur(),this.document.one("mousemove",function(){t(e.target).trigger(e.originalEvent)}),void 0):(n=i.item.data("ui-autocomplete-item"),!1!==this._trigger("focus",e,{item:n})&&e.originalEvent&&/^key/.test(e.originalEvent.type)&&this._value(n.value),s=i.item.attr("aria-label")||n.value,s&&t.trim(s).length&&(this.liveRegion.children().hide(),t("<div>").text(s).appendTo(this.liveRegion)),void 0)},menuselect:function(e,i){var s=i.item.data("ui-autocomplete-item"),n=this.previous;this.element[0]!==t.ui.safeActiveElement(this.document[0])&&(this.element.trigger("focus"),this.previous=n,this._delay(function(){this.previous=n,this.selectedItem=s})),!1!==this._trigger("select",e,{item:s})&&this._value(s.value),this.term=this._value(),this.close(e),this.selectedItem=s}}),this.liveRegion=t("<div>",{role:"status","aria-live":"assertive","aria-relevant":"additions"}).appendTo(this.document[0].body),this._addClass(this.liveRegion,null,"ui-helper-hidden-accessible"),this._on(this.window,{beforeunload:function(){this.element.removeAttr("autocomplete")}})},_destroy:function(){clearTimeout(this.searching),this.element.removeAttr("autocomplete"),this.menu.element.remove(),this.liveRegion.remove()},_setOption:function(t,e){this._super(t,e),"source"===t&&this._initSource(),"appendTo"===t&&this.menu.element.appendTo(this._appendTo()),"disabled"===t&&e&&this.xhr&&this.xhr.abort()},_isEventTargetInWidget:function(e){var i=this.menu.element[0];return e.target===this.element[0]||e.target===i||t.contains(i,e.target)},_closeOnClickOutside:function(t){this._isEventTargetInWidget(t)||this.close()},_appendTo:function(){var e=this.options.appendTo;return e&&(e=e.jquery||e.nodeType?t(e):this.document.find(e).eq(0)),e&&e[0]||(e=this.element.closest(".ui-front, dialog")),e.length||(e=this.document[0].body),e},_initSource:function(){var e,i,s=this;t.isArray(this.options.source)?(e=this.options.source,this.source=function(i,s){s(t.ui.autocomplete.filter(e,i.term))}):"string"==typeof this.options.source?(i=this.options.source,this.source=function(e,n){s.xhr&&s.xhr.abort(),s.xhr=t.ajax({url:i,data:e,dataType:"json",success:function(t){n(t)},error:function(){n([])}})}):this.source=this.options.source},_searchTimeout:function(t){clearTimeout(this.searching),this.searching=this._delay(function(){var e=this.term===this._value(),i=this.menu.element.is(":visible"),s=t.altKey||t.ctrlKey||t.metaKey||t.shiftKey;(!e||e&&!i&&!s)&&(this.selectedItem=null,this.search(null,t))},this.options.delay)},search:function(t,e){return t=null!=t?t:this._value(),this.term=this._value(),t.length<this.options.minLength?this.close(e):this._trigger("search",e)!==!1?this._search(t):void 0},_search:function(t){this.pending++,this._addClass("ui-autocomplete-loading"),this.cancelSearch=!1,this.source({term:t},this._response())},_response:function(){var e=++this.requestIndex;return t.proxy(function(t){e===this.requestIndex&&this.__response(t),this.pending--,this.pending||this._removeClass("ui-autocomplete-loading")},this)},__response:function(t){t&&(t=this._normalize(t)),this._trigger("response",null,{content:t}),!this.options.disabled&&t&&t.length&&!this.cancelSearch?(this._suggest(t),this._trigger("open")):this._close()},close:function(t){this.cancelSearch=!0,this._close(t)},_close:function(t){this._off(this.document,"mousedown"),this.menu.element.is(":visible")&&(this.menu.element.hide(),this.menu.blur(),this.isNewMenu=!0,this._trigger("close",t))},_change:function(t){this.previous!==this._value()&&this._trigger("change",t,{item:this.selectedItem})},_normalize:function(e){return e.length&&e[0].label&&e[0].value?e:t.map(e,function(e){return"string"==typeof e?{label:e,value:e}:t.extend({},e,{label:e.label||e.value,value:e.value||e.label})})},_suggest:function(e){var i=this.menu.element.empty();this._renderMenu(i,e),this.isNewMenu=!0,this.menu.refresh(),i.show(),this._resizeMenu(),i.position(t.extend({of:this.element},this.options.position)),this.options.autoFocus&&this.menu.next(),this._on(this.document,{mousedown:"_closeOnClickOutside"})},_resizeMenu:function(){var t=this.menu.element;t.outerWidth(Math.max(t.width("").outerWidth()+1,this.element.outerWidth()))},_renderMenu:function(e,i){var s=this;t.each(i,function(t,i){s._renderItemData(e,i)})},_renderItemData:function(t,e){return this._renderItem(t,e).data("ui-autocomplete-item",e)},_renderItem:function(e,i){return t("<li>").append(t("<div>").text(i.label)).appendTo(e)},_move:function(t,e){return this.menu.element.is(":visible")?this.menu.isFirstItem()&&/^previous/.test(t)||this.menu.isLastItem()&&/^next/.test(t)?(this.isMultiLine||this._value(this.term),this.menu.blur(),void 0):(this.menu[t](e),void 0):(this.search(null,e),void 0)},widget:function(){return this.menu.element},_value:function(){return this.valueMethod.apply(this.element,arguments)},_keyEvent:function(t,e){(!this.isMultiLine||this.menu.element.is(":visible"))&&(this._move(t,e),e.preventDefault())},_isContentEditable:function(t){if(!t.length)return!1;var e=t.prop("contentEditable");return"inherit"===e?this._isContentEditable(t.parent()):"true"===e}}),t.extend(t.ui.autocomplete,{escapeRegex:function(t){return t.replace(/[\-\[\]{}()*+?.,\\\^$|#\s]/g,"\\$&")},filter:function(e,i){var s=RegExp(t.ui.autocomplete.escapeRegex(i),"i");return t.grep(e,function(t){return s.test(t.label||t.value||t)})}}),t.widget("ui.autocomplete",t.ui.autocomplete,{options:{messages:{noResults:"No search results.",results:function(t){return t+(t>1?" results are":" result is")+" available, use up and down arrow keys to navigate."}}},__response:function(e){var i;this._superApply(arguments),this.options.disabled||this.cancelSearch||(i=e&&e.length?this.options.messages.results(e.length):this.options.messages.noResults,this.liveRegion.children().hide(),t("<div>").text(i).appendTo(this.liveRegion))}}),t.ui.autocomplete;var g=/ui-corner-([a-z]){2,6}/g;t.widget("ui.controlgroup",{version:"1.12.0",defaultElement:"<div>",options:{direction:"horizontal",disabled:null,onlyVisible:!0,items:{button:"input[type=button], input[type=submit], input[type=reset], button, a",controlgroupLabel:".ui-controlgroup-label",checkboxradio:"input[type='checkbox'], input[type='radio']",selectmenu:"select",spinner:".ui-spinner-input"}},_create:function(){this._enhance()},_enhance:function(){this.element.attr("role","toolbar"),this.refresh()},_destroy:function(){this._callChildMethod("destroy"),this.childWidgets.removeData("ui-controlgroup-data"),this.element.removeAttr("role"),this.options.items.controlgroupLabel&&this.element.find(this.options.items.controlgroupLabel).find(".ui-controlgroup-label-contents").contents().unwrap()},_initWidgets:function(){var e=this,i=[];t.each(this.options.items,function(s,n){var o,a={};return n?"controlgroupLabel"===s?(o=e.element.find(n),o.each(function(){var e=t(this);e.children(".ui-controlgroup-label-contents").length||e.contents().wrapAll("<span class='ui-controlgroup-label-contents'></span>")}),e._addClass(o,null,"ui-widget ui-widget-content ui-state-default"),i=i.concat(o.get()),void 0):(t.fn[s]&&(e["_"+s+"Options"]&&(a=e["_"+s+"Options"]("middle")),e.element.find(n).each(function(){var n=t(this),o=n[s]("instance"),r=t.widget.extend({},a);if("button"!==s||!n.parent(".ui-spinner").length){o||(o=n[s]()[s]("instance")),o&&(r.classes=e._resolveClassesValues(r.classes,o)),n[s](r);var h=n[s]("widget");t.data(h[0],"ui-controlgroup-data",o?o:n[s]("instance")),i.push(h[0])}})),void 0):void 0}),this.childWidgets=t(t.unique(i)),this._addClass(this.childWidgets,"ui-controlgroup-item")},_callChildMethod:function(e){this.childWidgets.each(function(){var i=t(this),s=i.data("ui-controlgroup-data");s&&s[e]&&s[e]()})},_updateCornerClass:function(t,e){var i="ui-corner-top ui-corner-bottom ui-corner-left ui-corner-right ui-corner-all",s=this._buildSimpleOptions(e,"label").classes.label;this._removeClass(t,null,i),this._addClass(t,null,s)},_buildSimpleOptions:function(t,e){var i="vertical"===this.options.direction,s={classes:{}};return s.classes[e]={middle:"",first:"ui-corner-"+(i?"top":"left"),last:"ui-corner-"+(i?"bottom":"right"),only:"ui-corner-all"}[t],s},_spinnerOptions:function(t){var e=this._buildSimpleOptions(t,"ui-spinner");return e.classes["ui-spinner-up"]="",e.classes["ui-spinner-down"]="",e},_buttonOptions:function(t){return this._buildSimpleOptions(t,"ui-button")},_checkboxradioOptions:function(t){return this._buildSimpleOptions(t,"ui-checkboxradio-label")},_selectmenuOptions:function(t){var e="vertical"===this.options.direction;return{width:e?"auto":!1,classes:{middle:{"ui-selectmenu-button-open":"","ui-selectmenu-button-closed":""},first:{"ui-selectmenu-button-open":"ui-corner-"+(e?"top":"tl"),"ui-selectmenu-button-closed":"ui-corner-"+(e?"top":"left")},last:{"ui-selectmenu-button-open":e?"":"ui-corner-tr","ui-selectmenu-button-closed":"ui-corner-"+(e?"bottom":"right")},only:{"ui-selectmenu-button-open":"ui-corner-top","ui-selectmenu-button-closed":"ui-corner-all"}}[t]}},_resolveClassesValues:function(e,i){var s={};return t.each(e,function(t){var n=i.options.classes[t]||"";n=n.replace(g,"").trim(),s[t]=(n+" "+e[t]).replace(/\s+/g," ")}),s},_setOption:function(t,e){return"direction"===t&&this._removeClass("ui-controlgroup-"+this.options.direction),this._super(t,e),"disabled"===t?(this._callChildMethod(e?"disable":"enable"),void 0):(this.refresh(),void 0)},refresh:function(){var e,i=this;this._addClass("ui-controlgroup ui-controlgroup-"+this.options.direction),"horizontal"===this.options.direction&&this._addClass(null,"ui-helper-clearfix"),this._initWidgets(),e=this.childWidgets,this.options.onlyVisible&&(e=e.filter(":visible")),e.length&&(t.each(["first","last"],function(t,s){var n=e[s]().data("ui-controlgroup-data");if(n&&i["_"+n.widgetName+"Options"]){var o=i["_"+n.widgetName+"Options"](1===e.length?"only":s);o.classes=i._resolveClassesValues(o.classes,n),n.element[n.widgetName](o)}else i._updateCornerClass(e[s](),s)}),this._callChildMethod("refresh"))}}),t.widget("ui.checkboxradio",[t.ui.formResetMixin,{version:"1.12.0",options:{disabled:null,label:null,icon:!0,classes:{"ui-checkboxradio-label":"ui-corner-all","ui-checkboxradio-icon":"ui-corner-all"}},_getCreateOptions:function(){var e,i,s=this,n=this._super()||{};return this._readType(),i=this.element.labels(),this.label=t(i[i.length-1]),this.label.length||t.error("No label found for checkboxradio widget"),this.originalLabel="",this.label.contents().not(this.element).each(function(){s.originalLabel+=3===this.nodeType?t(this).text():this.outerHTML}),this.originalLabel&&(n.label=this.originalLabel),e=this.element[0].disabled,null!=e&&(n.disabled=e),n},_create:function(){var t=this.element[0].checked;this._bindFormResetHandler(),null==this.options.disabled&&(this.options.disabled=this.element[0].disabled),this._setOption("disabled",this.options.disabled),this._addClass("ui-checkboxradio","ui-helper-hidden-accessible"),this._addClass(this.label,"ui-checkboxradio-label","ui-button ui-widget"),"radio"===this.type&&this._addClass(this.label,"ui-checkboxradio-radio-label"),this.options.label&&this.options.label!==this.originalLabel?this._updateLabel():this.originalLabel&&(this.options.label=this.originalLabel),this._enhance(),t&&(this._addClass(this.label,"ui-checkboxradio-checked","ui-state-active"),this.icon&&this._addClass(this.icon,null,"ui-state-hover")),this._on({change:"_toggleClasses",focus:function(){this._addClass(this.label,null,"ui-state-focus ui-visual-focus")},blur:function(){this._removeClass(this.label,null,"ui-state-focus ui-visual-focus")}})},_readType:function(){var e=this.element[0].nodeName.toLowerCase();this.type=this.element[0].type,"input"===e&&/radio|checkbox/.test(this.type)||t.error("Can't create checkboxradio on element.nodeName="+e+" and element.type="+this.type)},_enhance:function(){this._updateIcon(this.element[0].checked)},widget:function(){return this.label},_getRadioGroup:function(){var e,i=this.element[0].name,s="input[name='"+t.ui.escapeSelector(i)+"']";return i?(e=this.form.length?t(this.form[0].elements).filter(s):t(s).filter(function(){return 0===t(this).form().length}),e.not(this.element)):t([])},_toggleClasses:function(){var e=this.element[0].checked;this._toggleClass(this.label,"ui-checkboxradio-checked","ui-state-active",e),this.options.icon&&"checkbox"===this.type&&this._toggleClass(this.icon,null,"ui-icon-check ui-state-checked",e)._toggleClass(this.icon,null,"ui-icon-blank",!e),"radio"===this.type&&this._getRadioGroup().each(function(){var e=t(this).checkboxradio("instance");e&&e._removeClass(e.label,"ui-checkboxradio-checked","ui-state-active")})},_destroy:function(){this._unbindFormResetHandler(),this.icon&&(this.icon.remove(),this.iconSpace.remove())},_setOption:function(t,e){return"label"!==t||e?(this._super(t,e),"disabled"===t?(this._toggleClass(this.label,null,"ui-state-disabled",e),this.element[0].disabled=e,void 0):(this.refresh(),void 0)):void 0},_updateIcon:function(e){var i="ui-icon ui-icon-background ";this.options.icon?(this.icon||(this.icon=t("<span>"),this.iconSpace=t("<span> </span>"),this._addClass(this.iconSpace,"ui-checkboxradio-icon-space")),"checkbox"===this.type?(i+=e?"ui-icon-check ui-state-checked":"ui-icon-blank",this._removeClass(this.icon,null,e?"ui-icon-blank":"ui-icon-check")):i+="ui-icon-blank",this._addClass(this.icon,"ui-checkboxradio-icon",i),e||this._removeClass(this.icon,null,"ui-icon-check ui-state-checked"),this.icon.prependTo(this.label).after(this.iconSpace)):void 0!==this.icon&&(this.icon.remove(),this.iconSpace.remove(),delete this.icon)},_updateLabel:function(){this.label.contents().not(this.element.add(this.icon).add(this.iconSpace)).remove(),this.label.append(this.options.label)},refresh:function(){var t=this.element[0].checked,e=this.element[0].disabled;this._updateIcon(t),this._toggleClass(this.label,"ui-checkboxradio-checked","ui-state-active",t),null!==this.options.label&&this._updateLabel(),e!==this.options.disabled&&this._setOptions({disabled:e})}}]),t.ui.checkboxradio,t.widget("ui.button",{version:"1.12.0",defaultElement:"<button>",options:{classes:{"ui-button":"ui-corner-all"},disabled:null,icon:null,iconPosition:"beginning",label:null,showLabel:!0},_getCreateOptions:function(){var t,e=this._super()||{};return this.isInput=this.element.is("input"),t=this.element[0].disabled,null!=t&&(e.disabled=t),this.originalLabel=this.isInput?this.element.val():this.element.html(),this.originalLabel&&(e.label=this.originalLabel),e},_create:function(){!this.option.showLabel&!this.options.icon&&(this.options.showLabel=!0),null==this.options.disabled&&(this.options.disabled=this.element[0].disabled||!1),this.hasTitle=!!this.element.attr("title"),this.options.label&&this.options.label!==this.originalLabel&&(this.isInput?this.element.val(this.options.label):this.element.html(this.options.label)),this._addClass("ui-button","ui-widget"),this._setOption("disabled",this.options.disabled),this._enhance(),this.element.is("a")&&this._on({keyup:function(e){e.keyCode===t.ui.keyCode.SPACE&&(e.preventDefault(),this.element[0].click?this.element[0].click():this.element.trigger("click"))}})},_enhance:function(){this.element.is("button")||this.element.attr("role","button"),this.options.icon&&(this._updateIcon("icon",this.options.icon),this._updateTooltip())},_updateTooltip:function(){this.title=this.element.attr("title"),this.options.showLabel||this.title||this.element.attr("title",this.options.label)},_updateIcon:function(e,i){var s="iconPosition"!==e,n=s?this.options.iconPosition:i,o="top"===n||"bottom"===n;this.icon?s&&this._removeClass(this.icon,null,this.options.icon):(this.icon=t("<span>"),this._addClass(this.icon,"ui-button-icon","ui-icon"),this.options.showLabel||this._addClass("ui-button-icon-only")),s&&this._addClass(this.icon,null,i),this._attachIcon(n),o?(this._addClass(this.icon,null,"ui-widget-icon-block"),this.iconSpace&&this.iconSpace.remove()):(this.iconSpace||(this.iconSpace=t("<span> </span>"),this._addClass(this.iconSpace,"ui-button-icon-space")),this._removeClass(this.icon,null,"ui-wiget-icon-block"),this._attachIconSpace(n))},_destroy:function(){this.element.removeAttr("role"),this.icon&&this.icon.remove(),this.iconSpace&&this.iconSpace.remove(),this.hasTitle||this.element.removeAttr("title")},_attachIconSpace:function(t){this.icon[/^(?:end|bottom)/.test(t)?"before":"after"](this.iconSpace)},_attachIcon:function(t){this.element[/^(?:end|bottom)/.test(t)?"append":"prepend"](this.icon)},_setOptions:function(t){var e=void 0===t.showLabel?this.options.showLabel:t.showLabel,i=void 0===t.icon?this.options.icon:t.icon;e||i||(t.showLabel=!0),this._super(t)},_setOption:function(t,e){"icon"===t&&(e?this._updateIcon(t,e):this.icon&&(this.icon.remove(),this.iconSpace&&this.iconSpace.remove())),"iconPosition"===t&&this._updateIcon(t,e),"showLabel"===t&&(this._toggleClass("ui-button-icon-only",null,!e),this._updateTooltip()),"label"===t&&(this.isInput?this.element.val(e):(this.element.html(e),this.icon&&(this._attachIcon(this.options.iconPosition),this._attachIconSpace(this.options.iconPosition)))),this._super(t,e),"disabled"===t&&(this._toggleClass(null,"ui-state-disabled",e),this.element[0].disabled=e,e&&this.element.blur())},refresh:function(){var t=this.element.is("input, button")?this.element[0].disabled:this.element.hasClass("ui-button-disabled");t!==this.options.disabled&&this._setOptions({disabled:t}),this._updateTooltip()}}),t.uiBackCompat!==!1&&(t.widget("ui.button",t.ui.button,{options:{text:!0,icons:{primary:null,secondary:null}},_create:function(){this.options.showLabel&&!this.options.text&&(this.options.showLabel=this.options.text),!this.options.showLabel&&this.options.text&&(this.options.text=this.options.showLabel),this.options.icon||!this.options.icons.primary&&!this.options.icons.secondary?this.options.icon&&(this.options.icons.primary=this.options.icon):this.options.icons.primary?this.options.icon=this.options.icons.primary:(this.options.icon=this.options.icons.secondary,this.options.iconPosition="end"),this._super()},_setOption:function(t,e){return"text"===t?(this._super("showLabel",e),void 0):("showLabel"===t&&(this.options.text=e),"icon"===t&&(this.options.icons.primary=e),"icons"===t&&(e.primary?(this._super("icon",e.primary),this._super("iconPosition","beginning")):e.secondary&&(this._super("icon",e.secondary),this._super("iconPosition","end"))),this._superApply(arguments),void 0)}}),t.fn.button=function(e){return function(){return!this.length||this.length&&"INPUT"!==this[0].tagName||this.length&&"INPUT"===this[0].tagName&&"checkbox"!==this.attr("type")&&"radio"!==this.attr("type")?e.apply(this,arguments):(t.ui.checkboxradio||t.error("Checkboxradio widget missing"),0===arguments.length?this.checkboxradio({icon:!1}):this.checkboxradio.apply(this,arguments))}}(t.fn.button),t.fn.buttonset=function(){return t.ui.controlgroup||t.error("Controlgroup widget missing"),"option"===arguments[0]&&"items"===arguments[1]&&arguments[2]?this.controlgroup.apply(this,[arguments[0],"items.button",arguments[2]]):"option"===arguments[0]&&"items"===arguments[1]?this.controlgroup.apply(this,[arguments[0],"items.button"]):("object"==typeof arguments[0]&&arguments[0].items&&(arguments[0].items={button:arguments[0].items}),this.controlgroup.apply(this,arguments))}),t.ui.button,t.extend(t.ui,{datepicker:{version:"1.12.0"}});var m;t.extend(s.prototype,{markerClassName:"hasDatepicker",maxRows:4,_widgetDatepicker:function(){return this.dpDiv},setDefaults:function(t){return a(this._defaults,t||{}),this},_attachDatepicker:function(e,i){var s,n,o;s=e.nodeName.toLowerCase(),n="div"===s||"span"===s,e.id||(this.uuid+=1,e.id="dp"+this.uuid),o=this._newInst(t(e),n),o.settings=t.extend({},i||{}),"input"===s?this._connectDatepicker(e,o):n&&this._inlineDatepicker(e,o)},_newInst:function(e,i){var s=e[0].id.replace(/([^A-Za-z0-9_\-])/g,"\\\\$1");return{id:s,input:e,selectedDay:0,selectedMonth:0,selectedYear:0,drawMonth:0,drawYear:0,inline:i,dpDiv:i?n(t("<div class='"+this._inlineClass+" ui-datepicker ui-widget ui-widget-content ui-helper-clearfix ui-corner-all'></div>")):this.dpDiv}},_connectDatepicker:function(e,i){var s=t(e);i.append=t([]),i.trigger=t([]),s.hasClass(this.markerClassName)||(this._attachments(s,i),s.addClass(this.markerClassName).on("keydown",this._doKeyDown).on("keypress",this._doKeyPress).on("keyup",this._doKeyUp),this._autoSize(i),t.data(e,"datepicker",i),i.settings.disabled&&this._disableDatepicker(e))},_attachments:function(e,i){var s,n,o,a=this._get(i,"appendText"),r=this._get(i,"isRTL");i.append&&i.append.remove(),a&&(i.append=t("<span class='"+this._appendClass+"'>"+a+"</span>"),e[r?"before":"after"](i.append)),e.off("focus",this._showDatepicker),i.trigger&&i.trigger.remove(),s=this._get(i,"showOn"),("focus"===s||"both"===s)&&e.on("focus",this._showDatepicker),("button"===s||"both"===s)&&(n=this._get(i,"buttonText"),o=this._get(i,"buttonImage"),i.trigger=t(this._get(i,"buttonImageOnly")?t("<img/>").addClass(this._triggerClass).attr({src:o,alt:n,title:n}):t("<button type='button'></button>").addClass(this._triggerClass).html(o?t("<img/>").attr({src:o,alt:n,title:n}):n)),e[r?"before":"after"](i.trigger),i.trigger.on("click",function(){return t.datepicker._datepickerShowing&&t.datepicker._lastInput===e[0]?t.datepicker._hideDatepicker():t.datepicker._datepickerShowing&&t.datepicker._lastInput!==e[0]?(t.datepicker._hideDatepicker(),t.datepicker._showDatepicker(e[0])):t.datepicker._showDatepicker(e[0]),!1}))},_autoSize:function(t){if(this._get(t,"autoSize")&&!t.inline){var e,i,s,n,o=new Date(2009,11,20),a=this._get(t,"dateFormat");a.match(/[DM]/)&&(e=function(t){for(i=0,s=0,n=0;t.length>n;n++)t[n].length>i&&(i=t[n].length,s=n);return s},o.setMonth(e(this._get(t,a.match(/MM/)?"monthNames":"monthNamesShort"))),o.setDate(e(this._get(t,a.match(/DD/)?"dayNames":"dayNamesShort"))+20-o.getDay())),t.input.attr("size",this._formatDate(t,o).length)}},_inlineDatepicker:function(e,i){var s=t(e);s.hasClass(this.markerClassName)||(s.addClass(this.markerClassName).append(i.dpDiv),t.data(e,"datepicker",i),this._setDate(i,this._getDefaultDate(i),!0),this._updateDatepicker(i),this._updateAlternate(i),i.settings.disabled&&this._disableDatepicker(e),i.dpDiv.css("display","block"))},_dialogDatepicker:function(e,i,s,n,o){var r,h,l,c,u,d=this._dialogInst;return d||(this.uuid+=1,r="dp"+this.uuid,this._dialogInput=t("<input type='text' id='"+r+"' style='position: absolute; top: -100px; width: 0px;'/>"),this._dialogInput.on("keydown",this._doKeyDown),t("body").append(this._dialogInput),d=this._dialogInst=this._newInst(this._dialogInput,!1),d.settings={},t.data(this._dialogInput[0],"datepicker",d)),a(d.settings,n||{}),i=i&&i.constructor===Date?this._formatDate(d,i):i,this._dialogInput.val(i),this._pos=o?o.length?o:[o.pageX,o.pageY]:null,this._pos||(h=document.documentElement.clientWidth,l=document.documentElement.clientHeight,c=document.documentElement.scrollLeft||document.body.scrollLeft,u=document.documentElement.scrollTop||document.body.scrollTop,this._pos=[h/2-100+c,l/2-150+u]),this._dialogInput.css("left",this._pos[0]+20+"px").css("top",this._pos[1]+"px"),d.settings.onSelect=s,this._inDialog=!0,this.dpDiv.addClass(this._dialogClass),this._showDatepicker(this._dialogInput[0]),t.blockUI&&t.blockUI(this.dpDiv),t.data(this._dialogInput[0],"datepicker",d),this},_destroyDatepicker:function(e){var i,s=t(e),n=t.data(e,"datepicker");s.hasClass(this.markerClassName)&&(i=e.nodeName.toLowerCase(),t.removeData(e,"datepicker"),"input"===i?(n.append.remove(),n.trigger.remove(),s.removeClass(this.markerClassName).off("focus",this._showDatepicker).off("keydown",this._doKeyDown).off("keypress",this._doKeyPress).off("keyup",this._doKeyUp)):("div"===i||"span"===i)&&s.removeClass(this.markerClassName).empty(),m===n&&(m=null))},_enableDatepicker:function(e){var i,s,n=t(e),o=t.data(e,"datepicker");n.hasClass(this.markerClassName)&&(i=e.nodeName.toLowerCase(),"input"===i?(e.disabled=!1,o.trigger.filter("button").each(function(){this.disabled=!1}).end().filter("img").css({opacity:"1.0",cursor:""})):("div"===i||"span"===i)&&(s=n.children("."+this._inlineClass),s.children().removeClass("ui-state-disabled"),s.find("select.ui-datepicker-month, select.ui-datepicker-year").prop("disabled",!1)),this._disabledInputs=t.map(this._disabledInputs,function(t){return t===e?null:t}))},_disableDatepicker:function(e){var i,s,n=t(e),o=t.data(e,"datepicker");n.hasClass(this.markerClassName)&&(i=e.nodeName.toLowerCase(),"input"===i?(e.disabled=!0,o.trigger.filter("button").each(function(){this.disabled=!0}).end().filter("img").css({opacity:"0.5",cursor:"default"})):("div"===i||"span"===i)&&(s=n.children("."+this._inlineClass),s.children().addClass("ui-state-disabled"),s.find("select.ui-datepicker-month, select.ui-datepicker-year").prop("disabled",!0)),this._disabledInputs=t.map(this._disabledInputs,function(t){return t===e?null:t}),this._disabledInputs[this._disabledInputs.length]=e)},_isDisabledDatepicker:function(t){if(!t)return!1;for(var e=0;this._disabledInputs.length>e;e++)if(this._disabledInputs[e]===t)return!0;return!1},_getInst:function(e){try{return t.data(e,"datepicker")}catch(i){throw"Missing instance data for this datepicker"}},_optionDatepicker:function(e,i,s){var n,o,r,h,l=this._getInst(e);return 2===arguments.length&&"string"==typeof i?"defaults"===i?t.extend({},t.datepicker._defaults):l?"all"===i?t.extend({},l.settings):this._get(l,i):null:(n=i||{},"string"==typeof i&&(n={},n[i]=s),l&&(this._curInst===l&&this._hideDatepicker(),o=this._getDateDatepicker(e,!0),r=this._getMinMaxDate(l,"min"),h=this._getMinMaxDate(l,"max"),a(l.settings,n),null!==r&&void 0!==n.dateFormat&&void 0===n.minDate&&(l.settings.minDate=this._formatDate(l,r)),null!==h&&void 0!==n.dateFormat&&void 0===n.maxDate&&(l.settings.maxDate=this._formatDate(l,h)),"disabled"in n&&(n.disabled?this._disableDatepicker(e):this._enableDatepicker(e)),this._attachments(t(e),l),this._autoSize(l),this._setDate(l,o),this._updateAlternate(l),this._updateDatepicker(l)),void 0)},_changeDatepicker:function(t,e,i){this._optionDatepicker(t,e,i)},_refreshDatepicker:function(t){var e=this._getInst(t);e&&this._updateDatepicker(e)},_setDateDatepicker:function(t,e){var i=this._getInst(t);i&&(this._setDate(i,e),this._updateDatepicker(i),this._updateAlternate(i))},_getDateDatepicker:function(t,e){var i=this._getInst(t);return i&&!i.inline&&this._setDateFromField(i,e),i?this._getDate(i):null},_doKeyDown:function(e){var i,s,n,o=t.datepicker._getInst(e.target),a=!0,r=o.dpDiv.is(".ui-datepicker-rtl");if(o._keyEvent=!0,t.datepicker._datepickerShowing)switch(e.keyCode){case 9:t.datepicker._hideDatepicker(),a=!1;break;case 13:return n=t("td."+t.datepicker._dayOverClass+":not(."+t.datepicker._currentClass+")",o.dpDiv),n[0]&&t.datepicker._selectDay(e.target,o.selectedMonth,o.selectedYear,n[0]),i=t.datepicker._get(o,"onSelect"),i?(s=t.datepicker._formatDate(o),i.apply(o.input?o.input[0]:null,[s,o])):t.datepicker._hideDatepicker(),!1;case 27:t.datepicker._hideDatepicker();break;case 33:t.datepicker._adjustDate(e.target,e.ctrlKey?-t.datepicker._get(o,"stepBigMonths"):-t.datepicker._get(o,"stepMonths"),"M");break;case 34:t.datepicker._adjustDate(e.target,e.ctrlKey?+t.datepicker._get(o,"stepBigMonths"):+t.datepicker._get(o,"stepMonths"),"M");break;case 35:(e.ctrlKey||e.metaKey)&&t.datepicker._clearDate(e.target),a=e.ctrlKey||e.metaKey;break;case 36:(e.ctrlKey||e.metaKey)&&t.datepicker._gotoToday(e.target),a=e.ctrlKey||e.metaKey;break;case 37:(e.ctrlKey||e.metaKey)&&t.datepicker._adjustDate(e.target,r?1:-1,"D"),a=e.ctrlKey||e.metaKey,e.originalEvent.altKey&&t.datepicker._adjustDate(e.target,e.ctrlKey?-t.datepicker._get(o,"stepBigMonths"):-t.datepicker._get(o,"stepMonths"),"M");break;case 38:(e.ctrlKey||e.metaKey)&&t.datepicker._adjustDate(e.target,-7,"D"),a=e.ctrlKey||e.metaKey;break;case 39:(e.ctrlKey||e.metaKey)&&t.datepicker._adjustDate(e.target,r?-1:1,"D"),a=e.ctrlKey||e.metaKey,e.originalEvent.altKey&&t.datepicker._adjustDate(e.target,e.ctrlKey?+t.datepicker._get(o,"stepBigMonths"):+t.datepicker._get(o,"stepMonths"),"M");break;case 40:(e.ctrlKey||e.metaKey)&&t.datepicker._adjustDate(e.target,7,"D"),a=e.ctrlKey||e.metaKey;break;default:a=!1}else 36===e.keyCode&&e.ctrlKey?t.datepicker._showDatepicker(this):a=!1;a&&(e.preventDefault(),e.stopPropagation())},_doKeyPress:function(e){var i,s,n=t.datepicker._getInst(e.target);return t.datepicker._get(n,"constrainInput")?(i=t.datepicker._possibleChars(t.datepicker._get(n,"dateFormat")),s=String.fromCharCode(null==e.charCode?e.keyCode:e.charCode),e.ctrlKey||e.metaKey||" ">s||!i||i.indexOf(s)>-1):void 0},_doKeyUp:function(e){var i,s=t.datepicker._getInst(e.target);if(s.input.val()!==s.lastVal)try{i=t.datepicker.parseDate(t.datepicker._get(s,"dateFormat"),s.input?s.input.val():null,t.datepicker._getFormatConfig(s)),i&&(t.datepicker._setDateFromField(s),t.datepicker._updateAlternate(s),t.datepicker._updateDatepicker(s))}catch(n){}return!0},_showDatepicker:function(e){if(e=e.target||e,"input"!==e.nodeName.toLowerCase()&&(e=t("input",e.parentNode)[0]),!t.datepicker._isDisabledDatepicker(e)&&t.datepicker._lastInput!==e){var s,n,o,r,h,l,c;s=t.datepicker._getInst(e),t.datepicker._curInst&&t.datepicker._curInst!==s&&(t.datepicker._curInst.dpDiv.stop(!0,!0),s&&t.datepicker._datepickerShowing&&t.datepicker._hideDatepicker(t.datepicker._curInst.input[0])),n=t.datepicker._get(s,"beforeShow"),o=n?n.apply(e,[e,s]):{},o!==!1&&(a(s.settings,o),s.lastVal=null,t.datepicker._lastInput=e,t.datepicker._setDateFromField(s),t.datepicker._inDialog&&(e.value=""),t.datepicker._pos||(t.datepicker._pos=t.datepicker._findPos(e),t.datepicker._pos[1]+=e.offsetHeight),r=!1,t(e).parents().each(function(){return r|="fixed"===t(this).css("position"),!r}),h={left:t.datepicker._pos[0],top:t.datepicker._pos[1]},t.datepicker._pos=null,s.dpDiv.empty(),s.dpDiv.css({position:"absolute",display:"block",top:"-1000px"}),t.datepicker._updateDatepicker(s),h=t.datepicker._checkOffset(s,h,r),s.dpDiv.css({position:t.datepicker._inDialog&&t.blockUI?"static":r?"fixed":"absolute",display:"none",left:h.left+"px",top:h.top+"px"}),s.inline||(l=t.datepicker._get(s,"showAnim"),c=t.datepicker._get(s,"duration"),s.dpDiv.css("z-index",i(t(e))+1),t.datepicker._datepickerShowing=!0,t.effects&&t.effects.effect[l]?s.dpDiv.show(l,t.datepicker._get(s,"showOptions"),c):s.dpDiv[l||"show"](l?c:null),t.datepicker._shouldFocusInput(s)&&s.input.trigger("focus"),t.datepicker._curInst=s)) | ||
+ | }},_updateDatepicker:function(e){this.maxRows=4,m=e,e.dpDiv.empty().append(this._generateHTML(e)),this._attachHandlers(e);var i,s=this._getNumberOfMonths(e),n=s[1],a=17,r=e.dpDiv.find("."+this._dayOverClass+" a");r.length>0&&o.apply(r.get(0)),e.dpDiv.removeClass("ui-datepicker-multi-2 ui-datepicker-multi-3 ui-datepicker-multi-4").width(""),n>1&&e.dpDiv.addClass("ui-datepicker-multi-"+n).css("width",a*n+"em"),e.dpDiv[(1!==s[0]||1!==s[1]?"add":"remove")+"Class"]("ui-datepicker-multi"),e.dpDiv[(this._get(e,"isRTL")?"add":"remove")+"Class"]("ui-datepicker-rtl"),e===t.datepicker._curInst&&t.datepicker._datepickerShowing&&t.datepicker._shouldFocusInput(e)&&e.input.trigger("focus"),e.yearshtml&&(i=e.yearshtml,setTimeout(function(){i===e.yearshtml&&e.yearshtml&&e.dpDiv.find("select.ui-datepicker-year:first").replaceWith(e.yearshtml),i=e.yearshtml=null},0))},_shouldFocusInput:function(t){return t.input&&t.input.is(":visible")&&!t.input.is(":disabled")&&!t.input.is(":focus")},_checkOffset:function(e,i,s){var n=e.dpDiv.outerWidth(),o=e.dpDiv.outerHeight(),a=e.input?e.input.outerWidth():0,r=e.input?e.input.outerHeight():0,h=document.documentElement.clientWidth+(s?0:t(document).scrollLeft()),l=document.documentElement.clientHeight+(s?0:t(document).scrollTop());return i.left-=this._get(e,"isRTL")?n-a:0,i.left-=s&&i.left===e.input.offset().left?t(document).scrollLeft():0,i.top-=s&&i.top===e.input.offset().top+r?t(document).scrollTop():0,i.left-=Math.min(i.left,i.left+n>h&&h>n?Math.abs(i.left+n-h):0),i.top-=Math.min(i.top,i.top+o>l&&l>o?Math.abs(o+r):0),i},_findPos:function(e){for(var i,s=this._getInst(e),n=this._get(s,"isRTL");e&&("hidden"===e.type||1!==e.nodeType||t.expr.filters.hidden(e));)e=e[n?"previousSibling":"nextSibling"];return i=t(e).offset(),[i.left,i.top]},_hideDatepicker:function(e){var i,s,n,o,a=this._curInst;!a||e&&a!==t.data(e,"datepicker")||this._datepickerShowing&&(i=this._get(a,"showAnim"),s=this._get(a,"duration"),n=function(){t.datepicker._tidyDialog(a)},t.effects&&(t.effects.effect[i]||t.effects[i])?a.dpDiv.hide(i,t.datepicker._get(a,"showOptions"),s,n):a.dpDiv["slideDown"===i?"slideUp":"fadeIn"===i?"fadeOut":"hide"](i?s:null,n),i||n(),this._datepickerShowing=!1,o=this._get(a,"onClose"),o&&o.apply(a.input?a.input[0]:null,[a.input?a.input.val():"",a]),this._lastInput=null,this._inDialog&&(this._dialogInput.css({position:"absolute",left:"0",top:"-100px"}),t.blockUI&&(t.unblockUI(),t("body").append(this.dpDiv))),this._inDialog=!1)},_tidyDialog:function(t){t.dpDiv.removeClass(this._dialogClass).off(".ui-datepicker-calendar")},_checkExternalClick:function(e){if(t.datepicker._curInst){var i=t(e.target),s=t.datepicker._getInst(i[0]);(i[0].id!==t.datepicker._mainDivId&&0===i.parents("#"+t.datepicker._mainDivId).length&&!i.hasClass(t.datepicker.markerClassName)&&!i.closest("."+t.datepicker._triggerClass).length&&t.datepicker._datepickerShowing&&(!t.datepicker._inDialog||!t.blockUI)||i.hasClass(t.datepicker.markerClassName)&&t.datepicker._curInst!==s)&&t.datepicker._hideDatepicker()}},_adjustDate:function(e,i,s){var n=t(e),o=this._getInst(n[0]);this._isDisabledDatepicker(n[0])||(this._adjustInstDate(o,i+("M"===s?this._get(o,"showCurrentAtPos"):0),s),this._updateDatepicker(o))},_gotoToday:function(e){var i,s=t(e),n=this._getInst(s[0]);this._get(n,"gotoCurrent")&&n.currentDay?(n.selectedDay=n.currentDay,n.drawMonth=n.selectedMonth=n.currentMonth,n.drawYear=n.selectedYear=n.currentYear):(i=new Date,n.selectedDay=i.getDate(),n.drawMonth=n.selectedMonth=i.getMonth(),n.drawYear=n.selectedYear=i.getFullYear()),this._notifyChange(n),this._adjustDate(s)},_selectMonthYear:function(e,i,s){var n=t(e),o=this._getInst(n[0]);o["selected"+("M"===s?"Month":"Year")]=o["draw"+("M"===s?"Month":"Year")]=parseInt(i.options[i.selectedIndex].value,10),this._notifyChange(o),this._adjustDate(n)},_selectDay:function(e,i,s,n){var o,a=t(e);t(n).hasClass(this._unselectableClass)||this._isDisabledDatepicker(a[0])||(o=this._getInst(a[0]),o.selectedDay=o.currentDay=t("a",n).html(),o.selectedMonth=o.currentMonth=i,o.selectedYear=o.currentYear=s,this._selectDate(e,this._formatDate(o,o.currentDay,o.currentMonth,o.currentYear)))},_clearDate:function(e){var i=t(e);this._selectDate(i,"")},_selectDate:function(e,i){var s,n=t(e),o=this._getInst(n[0]);i=null!=i?i:this._formatDate(o),o.input&&o.input.val(i),this._updateAlternate(o),s=this._get(o,"onSelect"),s?s.apply(o.input?o.input[0]:null,[i,o]):o.input&&o.input.trigger("change"),o.inline?this._updateDatepicker(o):(this._hideDatepicker(),this._lastInput=o.input[0],"object"!=typeof o.input[0]&&o.input.trigger("focus"),this._lastInput=null)},_updateAlternate:function(e){var i,s,n,o=this._get(e,"altField");o&&(i=this._get(e,"altFormat")||this._get(e,"dateFormat"),s=this._getDate(e),n=this.formatDate(i,s,this._getFormatConfig(e)),t(o).val(n))},noWeekends:function(t){var e=t.getDay();return[e>0&&6>e,""]},iso8601Week:function(t){var e,i=new Date(t.getTime());return i.setDate(i.getDate()+4-(i.getDay()||7)),e=i.getTime(),i.setMonth(0),i.setDate(1),Math.floor(Math.round((e-i)/864e5)/7)+1},parseDate:function(e,i,s){if(null==e||null==i)throw"Invalid arguments";if(i="object"==typeof i?""+i:i+"",""===i)return null;var n,o,a,r,h=0,l=(s?s.shortYearCutoff:null)||this._defaults.shortYearCutoff,c="string"!=typeof l?l:(new Date).getFullYear()%100+parseInt(l,10),u=(s?s.dayNamesShort:null)||this._defaults.dayNamesShort,d=(s?s.dayNames:null)||this._defaults.dayNames,p=(s?s.monthNamesShort:null)||this._defaults.monthNamesShort,f=(s?s.monthNames:null)||this._defaults.monthNames,g=-1,m=-1,_=-1,v=-1,b=!1,y=function(t){var i=e.length>n+1&&e.charAt(n+1)===t;return i&&n++,i},w=function(t){var e=y(t),s="@"===t?14:"!"===t?20:"y"===t&&e?4:"o"===t?3:2,n="y"===t?s:1,o=RegExp("^\\d{"+n+","+s+"}"),a=i.substring(h).match(o);if(!a)throw"Missing number at position "+h;return h+=a[0].length,parseInt(a[0],10)},k=function(e,s,n){var o=-1,a=t.map(y(e)?n:s,function(t,e){return[[e,t]]}).sort(function(t,e){return-(t[1].length-e[1].length)});if(t.each(a,function(t,e){var s=e[1];return i.substr(h,s.length).toLowerCase()===s.toLowerCase()?(o=e[0],h+=s.length,!1):void 0}),-1!==o)return o+1;throw"Unknown name at position "+h},x=function(){if(i.charAt(h)!==e.charAt(n))throw"Unexpected literal at position "+h;h++};for(n=0;e.length>n;n++)if(b)"'"!==e.charAt(n)||y("'")?x():b=!1;else switch(e.charAt(n)){case"d":_=w("d");break;case"D":k("D",u,d);break;case"o":v=w("o");break;case"m":m=w("m");break;case"M":m=k("M",p,f);break;case"y":g=w("y");break;case"@":r=new Date(w("@")),g=r.getFullYear(),m=r.getMonth()+1,_=r.getDate();break;case"!":r=new Date((w("!")-this._ticksTo1970)/1e4),g=r.getFullYear(),m=r.getMonth()+1,_=r.getDate();break;case"'":y("'")?x():b=!0;break;default:x()}if(i.length>h&&(a=i.substr(h),!/^\s+/.test(a)))throw"Extra/unparsed characters found in date: "+a;if(-1===g?g=(new Date).getFullYear():100>g&&(g+=(new Date).getFullYear()-(new Date).getFullYear()%100+(c>=g?0:-100)),v>-1)for(m=1,_=v;;){if(o=this._getDaysInMonth(g,m-1),o>=_)break;m++,_-=o}if(r=this._daylightSavingAdjust(new Date(g,m-1,_)),r.getFullYear()!==g||r.getMonth()+1!==m||r.getDate()!==_)throw"Invalid date";return r},ATOM:"yy-mm-dd",COOKIE:"D, dd M yy",ISO_8601:"yy-mm-dd",RFC_822:"D, d M y",RFC_850:"DD, dd-M-y",RFC_1036:"D, d M y",RFC_1123:"D, d M yy",RFC_2822:"D, d M yy",RSS:"D, d M y",TICKS:"!",TIMESTAMP:"@",W3C:"yy-mm-dd",_ticksTo1970:1e7*60*60*24*(718685+Math.floor(492.5)-Math.floor(19.7)+Math.floor(4.925)),formatDate:function(t,e,i){if(!e)return"";var s,n=(i?i.dayNamesShort:null)||this._defaults.dayNamesShort,o=(i?i.dayNames:null)||this._defaults.dayNames,a=(i?i.monthNamesShort:null)||this._defaults.monthNamesShort,r=(i?i.monthNames:null)||this._defaults.monthNames,h=function(e){var i=t.length>s+1&&t.charAt(s+1)===e;return i&&s++,i},l=function(t,e,i){var s=""+e;if(h(t))for(;i>s.length;)s="0"+s;return s},c=function(t,e,i,s){return h(t)?s[e]:i[e]},u="",d=!1;if(e)for(s=0;t.length>s;s++)if(d)"'"!==t.charAt(s)||h("'")?u+=t.charAt(s):d=!1;else switch(t.charAt(s)){case"d":u+=l("d",e.getDate(),2);break;case"D":u+=c("D",e.getDay(),n,o);break;case"o":u+=l("o",Math.round((new Date(e.getFullYear(),e.getMonth(),e.getDate()).getTime()-new Date(e.getFullYear(),0,0).getTime())/864e5),3);break;case"m":u+=l("m",e.getMonth()+1,2);break;case"M":u+=c("M",e.getMonth(),a,r);break;case"y":u+=h("y")?e.getFullYear():(10>e.getFullYear()%100?"0":"")+e.getFullYear()%100;break;case"@":u+=e.getTime();break;case"!":u+=1e4*e.getTime()+this._ticksTo1970;break;case"'":h("'")?u+="'":d=!0;break;default:u+=t.charAt(s)}return u},_possibleChars:function(t){var e,i="",s=!1,n=function(i){var s=t.length>e+1&&t.charAt(e+1)===i;return s&&e++,s};for(e=0;t.length>e;e++)if(s)"'"!==t.charAt(e)||n("'")?i+=t.charAt(e):s=!1;else switch(t.charAt(e)){case"d":case"m":case"y":case"@":i+="0123456789";break;case"D":case"M":return null;case"'":n("'")?i+="'":s=!0;break;default:i+=t.charAt(e)}return i},_get:function(t,e){return void 0!==t.settings[e]?t.settings[e]:this._defaults[e]},_setDateFromField:function(t,e){if(t.input.val()!==t.lastVal){var i=this._get(t,"dateFormat"),s=t.lastVal=t.input?t.input.val():null,n=this._getDefaultDate(t),o=n,a=this._getFormatConfig(t);try{o=this.parseDate(i,s,a)||n}catch(r){s=e?"":s}t.selectedDay=o.getDate(),t.drawMonth=t.selectedMonth=o.getMonth(),t.drawYear=t.selectedYear=o.getFullYear(),t.currentDay=s?o.getDate():0,t.currentMonth=s?o.getMonth():0,t.currentYear=s?o.getFullYear():0,this._adjustInstDate(t)}},_getDefaultDate:function(t){return this._restrictMinMax(t,this._determineDate(t,this._get(t,"defaultDate"),new Date))},_determineDate:function(e,i,s){var n=function(t){var e=new Date;return e.setDate(e.getDate()+t),e},o=function(i){try{return t.datepicker.parseDate(t.datepicker._get(e,"dateFormat"),i,t.datepicker._getFormatConfig(e))}catch(s){}for(var n=(i.toLowerCase().match(/^c/)?t.datepicker._getDate(e):null)||new Date,o=n.getFullYear(),a=n.getMonth(),r=n.getDate(),h=/([+\-]?[0-9]+)\s*(d|D|w|W|m|M|y|Y)?/g,l=h.exec(i);l;){switch(l[2]||"d"){case"d":case"D":r+=parseInt(l[1],10);break;case"w":case"W":r+=7*parseInt(l[1],10);break;case"m":case"M":a+=parseInt(l[1],10),r=Math.min(r,t.datepicker._getDaysInMonth(o,a));break;case"y":case"Y":o+=parseInt(l[1],10),r=Math.min(r,t.datepicker._getDaysInMonth(o,a))}l=h.exec(i)}return new Date(o,a,r)},a=null==i||""===i?s:"string"==typeof i?o(i):"number"==typeof i?isNaN(i)?s:n(i):new Date(i.getTime());return a=a&&"Invalid Date"==""+a?s:a,a&&(a.setHours(0),a.setMinutes(0),a.setSeconds(0),a.setMilliseconds(0)),this._daylightSavingAdjust(a)},_daylightSavingAdjust:function(t){return t?(t.setHours(t.getHours()>12?t.getHours()+2:0),t):null},_setDate:function(t,e,i){var s=!e,n=t.selectedMonth,o=t.selectedYear,a=this._restrictMinMax(t,this._determineDate(t,e,new Date));t.selectedDay=t.currentDay=a.getDate(),t.drawMonth=t.selectedMonth=t.currentMonth=a.getMonth(),t.drawYear=t.selectedYear=t.currentYear=a.getFullYear(),n===t.selectedMonth&&o===t.selectedYear||i||this._notifyChange(t),this._adjustInstDate(t),t.input&&t.input.val(s?"":this._formatDate(t))},_getDate:function(t){var e=!t.currentYear||t.input&&""===t.input.val()?null:this._daylightSavingAdjust(new Date(t.currentYear,t.currentMonth,t.currentDay));return e},_attachHandlers:function(e){var i=this._get(e,"stepMonths"),s="#"+e.id.replace(/\\\\/g,"\\");e.dpDiv.find("[data-handler]").map(function(){var e={prev:function(){t.datepicker._adjustDate(s,-i,"M")},next:function(){t.datepicker._adjustDate(s,+i,"M")},hide:function(){t.datepicker._hideDatepicker()},today:function(){t.datepicker._gotoToday(s)},selectDay:function(){return t.datepicker._selectDay(s,+this.getAttribute("data-month"),+this.getAttribute("data-year"),this),!1},selectMonth:function(){return t.datepicker._selectMonthYear(s,this,"M"),!1},selectYear:function(){return t.datepicker._selectMonthYear(s,this,"Y"),!1}};t(this).on(this.getAttribute("data-event"),e[this.getAttribute("data-handler")])})},_generateHTML:function(t){var e,i,s,n,o,a,r,h,l,c,u,d,p,f,g,m,_,v,b,y,w,k,x,C,D,I,T,P,M,S,H,z,O,A,N,W,E,F,R,L=new Date,B=this._daylightSavingAdjust(new Date(L.getFullYear(),L.getMonth(),L.getDate())),Y=this._get(t,"isRTL"),j=this._get(t,"showButtonPanel"),q=this._get(t,"hideIfNoPrevNext"),K=this._get(t,"navigationAsDateFormat"),U=this._getNumberOfMonths(t),V=this._get(t,"showCurrentAtPos"),X=this._get(t,"stepMonths"),$=1!==U[0]||1!==U[1],G=this._daylightSavingAdjust(t.currentDay?new Date(t.currentYear,t.currentMonth,t.currentDay):new Date(9999,9,9)),Q=this._getMinMaxDate(t,"min"),J=this._getMinMaxDate(t,"max"),Z=t.drawMonth-V,te=t.drawYear;if(0>Z&&(Z+=12,te--),J)for(e=this._daylightSavingAdjust(new Date(J.getFullYear(),J.getMonth()-U[0]*U[1]+1,J.getDate())),e=Q&&Q>e?Q:e;this._daylightSavingAdjust(new Date(te,Z,1))>e;)Z--,0>Z&&(Z=11,te--);for(t.drawMonth=Z,t.drawYear=te,i=this._get(t,"prevText"),i=K?this.formatDate(i,this._daylightSavingAdjust(new Date(te,Z-X,1)),this._getFormatConfig(t)):i,s=this._canAdjustMonth(t,-1,te,Z)?"<a class='ui-datepicker-prev ui-corner-all' data-handler='prev' data-event='click' title='"+i+"'><span class='ui-icon ui-icon-circle-triangle-"+(Y?"e":"w")+"'>"+i+"</span></a>":q?"":"<a class='ui-datepicker-prev ui-corner-all ui-state-disabled' title='"+i+"'><span class='ui-icon ui-icon-circle-triangle-"+(Y?"e":"w")+"'>"+i+"</span></a>",n=this._get(t,"nextText"),n=K?this.formatDate(n,this._daylightSavingAdjust(new Date(te,Z+X,1)),this._getFormatConfig(t)):n,o=this._canAdjustMonth(t,1,te,Z)?"<a class='ui-datepicker-next ui-corner-all' data-handler='next' data-event='click' title='"+n+"'><span class='ui-icon ui-icon-circle-triangle-"+(Y?"w":"e")+"'>"+n+"</span></a>":q?"":"<a class='ui-datepicker-next ui-corner-all ui-state-disabled' title='"+n+"'><span class='ui-icon ui-icon-circle-triangle-"+(Y?"w":"e")+"'>"+n+"</span></a>",a=this._get(t,"currentText"),r=this._get(t,"gotoCurrent")&&t.currentDay?G:B,a=K?this.formatDate(a,r,this._getFormatConfig(t)):a,h=t.inline?"":"<button type='button' class='ui-datepicker-close ui-state-default ui-priority-primary ui-corner-all' data-handler='hide' data-event='click'>"+this._get(t,"closeText")+"</button>",l=j?"<div class='ui-datepicker-buttonpane ui-widget-content'>"+(Y?h:"")+(this._isInRange(t,r)?"<button type='button' class='ui-datepicker-current ui-state-default ui-priority-secondary ui-corner-all' data-handler='today' data-event='click'>"+a+"</button>":"")+(Y?"":h)+"</div>":"",c=parseInt(this._get(t,"firstDay"),10),c=isNaN(c)?0:c,u=this._get(t,"showWeek"),d=this._get(t,"dayNames"),p=this._get(t,"dayNamesMin"),f=this._get(t,"monthNames"),g=this._get(t,"monthNamesShort"),m=this._get(t,"beforeShowDay"),_=this._get(t,"showOtherMonths"),v=this._get(t,"selectOtherMonths"),b=this._getDefaultDate(t),y="",k=0;U[0]>k;k++){for(x="",this.maxRows=4,C=0;U[1]>C;C++){if(D=this._daylightSavingAdjust(new Date(te,Z,t.selectedDay)),I=" ui-corner-all",T="",$){if(T+="<div class='ui-datepicker-group",U[1]>1)switch(C){case 0:T+=" ui-datepicker-group-first",I=" ui-corner-"+(Y?"right":"left");break;case U[1]-1:T+=" ui-datepicker-group-last",I=" ui-corner-"+(Y?"left":"right");break;default:T+=" ui-datepicker-group-middle",I=""}T+="'>"}for(T+="<div class='ui-datepicker-header ui-widget-header ui-helper-clearfix"+I+"'>"+(/all|left/.test(I)&&0===k?Y?o:s:"")+(/all|right/.test(I)&&0===k?Y?s:o:"")+this._generateMonthYearHeader(t,Z,te,Q,J,k>0||C>0,f,g)+"</div><table class='ui-datepicker-calendar'><thead>"+"<tr>",P=u?"<th class='ui-datepicker-week-col'>"+this._get(t,"weekHeader")+"</th>":"",w=0;7>w;w++)M=(w+c)%7,P+="<th scope='col'"+((w+c+6)%7>=5?" class='ui-datepicker-week-end'":"")+">"+"<span title='"+d[M]+"'>"+p[M]+"</span></th>";for(T+=P+"</tr></thead><tbody>",S=this._getDaysInMonth(te,Z),te===t.selectedYear&&Z===t.selectedMonth&&(t.selectedDay=Math.min(t.selectedDay,S)),H=(this._getFirstDayOfMonth(te,Z)-c+7)%7,z=Math.ceil((H+S)/7),O=$?this.maxRows>z?this.maxRows:z:z,this.maxRows=O,A=this._daylightSavingAdjust(new Date(te,Z,1-H)),N=0;O>N;N++){for(T+="<tr>",W=u?"<td class='ui-datepicker-week-col'>"+this._get(t,"calculateWeek")(A)+"</td>":"",w=0;7>w;w++)E=m?m.apply(t.input?t.input[0]:null,[A]):[!0,""],F=A.getMonth()!==Z,R=F&&!v||!E[0]||Q&&Q>A||J&&A>J,W+="<td class='"+((w+c+6)%7>=5?" ui-datepicker-week-end":"")+(F?" ui-datepicker-other-month":"")+(A.getTime()===D.getTime()&&Z===t.selectedMonth&&t._keyEvent||b.getTime()===A.getTime()&&b.getTime()===D.getTime()?" "+this._dayOverClass:"")+(R?" "+this._unselectableClass+" ui-state-disabled":"")+(F&&!_?"":" "+E[1]+(A.getTime()===G.getTime()?" "+this._currentClass:"")+(A.getTime()===B.getTime()?" ui-datepicker-today":""))+"'"+(F&&!_||!E[2]?"":" title='"+E[2].replace(/'/g,"'")+"'")+(R?"":" data-handler='selectDay' data-event='click' data-month='"+A.getMonth()+"' data-year='"+A.getFullYear()+"'")+">"+(F&&!_?" ":R?"<span class='ui-state-default'>"+A.getDate()+"</span>":"<a class='ui-state-default"+(A.getTime()===B.getTime()?" ui-state-highlight":"")+(A.getTime()===G.getTime()?" ui-state-active":"")+(F?" ui-priority-secondary":"")+"' href='#'>"+A.getDate()+"</a>")+"</td>",A.setDate(A.getDate()+1),A=this._daylightSavingAdjust(A);T+=W+"</tr>"}Z++,Z>11&&(Z=0,te++),T+="</tbody></table>"+($?"</div>"+(U[0]>0&&C===U[1]-1?"<div class='ui-datepicker-row-break'></div>":""):""),x+=T}y+=x}return y+=l,t._keyEvent=!1,y},_generateMonthYearHeader:function(t,e,i,s,n,o,a,r){var h,l,c,u,d,p,f,g,m=this._get(t,"changeMonth"),_=this._get(t,"changeYear"),v=this._get(t,"showMonthAfterYear"),b="<div class='ui-datepicker-title'>",y="";if(o||!m)y+="<span class='ui-datepicker-month'>"+a[e]+"</span>";else{for(h=s&&s.getFullYear()===i,l=n&&n.getFullYear()===i,y+="<select class='ui-datepicker-month' data-handler='selectMonth' data-event='change'>",c=0;12>c;c++)(!h||c>=s.getMonth())&&(!l||n.getMonth()>=c)&&(y+="<option value='"+c+"'"+(c===e?" selected='selected'":"")+">"+r[c]+"</option>");y+="</select>"}if(v||(b+=y+(!o&&m&&_?"":" ")),!t.yearshtml)if(t.yearshtml="",o||!_)b+="<span class='ui-datepicker-year'>"+i+"</span>";else{for(u=this._get(t,"yearRange").split(":"),d=(new Date).getFullYear(),p=function(t){var e=t.match(/c[+\-].*/)?i+parseInt(t.substring(1),10):t.match(/[+\-].*/)?d+parseInt(t,10):parseInt(t,10);return isNaN(e)?d:e},f=p(u[0]),g=Math.max(f,p(u[1]||"")),f=s?Math.max(f,s.getFullYear()):f,g=n?Math.min(g,n.getFullYear()):g,t.yearshtml+="<select class='ui-datepicker-year' data-handler='selectYear' data-event='change'>";g>=f;f++)t.yearshtml+="<option value='"+f+"'"+(f===i?" selected='selected'":"")+">"+f+"</option>";t.yearshtml+="</select>",b+=t.yearshtml,t.yearshtml=null}return b+=this._get(t,"yearSuffix"),v&&(b+=(!o&&m&&_?"":" ")+y),b+="</div>"},_adjustInstDate:function(t,e,i){var s=t.selectedYear+("Y"===i?e:0),n=t.selectedMonth+("M"===i?e:0),o=Math.min(t.selectedDay,this._getDaysInMonth(s,n))+("D"===i?e:0),a=this._restrictMinMax(t,this._daylightSavingAdjust(new Date(s,n,o)));t.selectedDay=a.getDate(),t.drawMonth=t.selectedMonth=a.getMonth(),t.drawYear=t.selectedYear=a.getFullYear(),("M"===i||"Y"===i)&&this._notifyChange(t)},_restrictMinMax:function(t,e){var i=this._getMinMaxDate(t,"min"),s=this._getMinMaxDate(t,"max"),n=i&&i>e?i:e;return s&&n>s?s:n},_notifyChange:function(t){var e=this._get(t,"onChangeMonthYear");e&&e.apply(t.input?t.input[0]:null,[t.selectedYear,t.selectedMonth+1,t])},_getNumberOfMonths:function(t){var e=this._get(t,"numberOfMonths");return null==e?[1,1]:"number"==typeof e?[1,e]:e},_getMinMaxDate:function(t,e){return this._determineDate(t,this._get(t,e+"Date"),null)},_getDaysInMonth:function(t,e){return 32-this._daylightSavingAdjust(new Date(t,e,32)).getDate()},_getFirstDayOfMonth:function(t,e){return new Date(t,e,1).getDay()},_canAdjustMonth:function(t,e,i,s){var n=this._getNumberOfMonths(t),o=this._daylightSavingAdjust(new Date(i,s+(0>e?e:n[0]*n[1]),1));return 0>e&&o.setDate(this._getDaysInMonth(o.getFullYear(),o.getMonth())),this._isInRange(t,o)},_isInRange:function(t,e){var i,s,n=this._getMinMaxDate(t,"min"),o=this._getMinMaxDate(t,"max"),a=null,r=null,h=this._get(t,"yearRange");return h&&(i=h.split(":"),s=(new Date).getFullYear(),a=parseInt(i[0],10),r=parseInt(i[1],10),i[0].match(/[+\-].*/)&&(a+=s),i[1].match(/[+\-].*/)&&(r+=s)),(!n||e.getTime()>=n.getTime())&&(!o||e.getTime()<=o.getTime())&&(!a||e.getFullYear()>=a)&&(!r||r>=e.getFullYear())},_getFormatConfig:function(t){var e=this._get(t,"shortYearCutoff");return e="string"!=typeof e?e:(new Date).getFullYear()%100+parseInt(e,10),{shortYearCutoff:e,dayNamesShort:this._get(t,"dayNamesShort"),dayNames:this._get(t,"dayNames"),monthNamesShort:this._get(t,"monthNamesShort"),monthNames:this._get(t,"monthNames")}},_formatDate:function(t,e,i,s){e||(t.currentDay=t.selectedDay,t.currentMonth=t.selectedMonth,t.currentYear=t.selectedYear);var n=e?"object"==typeof e?e:this._daylightSavingAdjust(new Date(s,i,e)):this._daylightSavingAdjust(new Date(t.currentYear,t.currentMonth,t.currentDay));return this.formatDate(this._get(t,"dateFormat"),n,this._getFormatConfig(t))}}),t.fn.datepicker=function(e){if(!this.length)return this;t.datepicker.initialized||(t(document).on("mousedown",t.datepicker._checkExternalClick),t.datepicker.initialized=!0),0===t("#"+t.datepicker._mainDivId).length&&t("body").append(t.datepicker.dpDiv);var i=Array.prototype.slice.call(arguments,1);return"string"!=typeof e||"isDisabled"!==e&&"getDate"!==e&&"widget"!==e?"option"===e&&2===arguments.length&&"string"==typeof arguments[1]?t.datepicker["_"+e+"Datepicker"].apply(t.datepicker,[this[0]].concat(i)):this.each(function(){"string"==typeof e?t.datepicker["_"+e+"Datepicker"].apply(t.datepicker,[this].concat(i)):t.datepicker._attachDatepicker(this,e)}):t.datepicker["_"+e+"Datepicker"].apply(t.datepicker,[this[0]].concat(i))},t.datepicker=new s,t.datepicker.initialized=!1,t.datepicker.uuid=(new Date).getTime(),t.datepicker.version="1.12.0",t.datepicker,t.ui.ie=!!/msie [\w.]+/.exec(navigator.userAgent.toLowerCase());var _=!1;t(document).on("mouseup",function(){_=!1}),t.widget("ui.mouse",{version:"1.12.0",options:{cancel:"input, textarea, button, select, option",distance:1,delay:0},_mouseInit:function(){var e=this;this.element.on("mousedown."+this.widgetName,function(t){return e._mouseDown(t)}).on("click."+this.widgetName,function(i){return!0===t.data(i.target,e.widgetName+".preventClickEvent")?(t.removeData(i.target,e.widgetName+".preventClickEvent"),i.stopImmediatePropagation(),!1):void 0}),this.started=!1},_mouseDestroy:function(){this.element.off("."+this.widgetName),this._mouseMoveDelegate&&this.document.off("mousemove."+this.widgetName,this._mouseMoveDelegate).off("mouseup."+this.widgetName,this._mouseUpDelegate)},_mouseDown:function(e){if(!_){this._mouseMoved=!1,this._mouseStarted&&this._mouseUp(e),this._mouseDownEvent=e;var i=this,s=1===e.which,n="string"==typeof this.options.cancel&&e.target.nodeName?t(e.target).closest(this.options.cancel).length:!1;return s&&!n&&this._mouseCapture(e)?(this.mouseDelayMet=!this.options.delay,this.mouseDelayMet||(this._mouseDelayTimer=setTimeout(function(){i.mouseDelayMet=!0},this.options.delay)),this._mouseDistanceMet(e)&&this._mouseDelayMet(e)&&(this._mouseStarted=this._mouseStart(e)!==!1,!this._mouseStarted)?(e.preventDefault(),!0):(!0===t.data(e.target,this.widgetName+".preventClickEvent")&&t.removeData(e.target,this.widgetName+".preventClickEvent"),this._mouseMoveDelegate=function(t){return i._mouseMove(t)},this._mouseUpDelegate=function(t){return i._mouseUp(t)},this.document.on("mousemove."+this.widgetName,this._mouseMoveDelegate).on("mouseup."+this.widgetName,this._mouseUpDelegate),e.preventDefault(),_=!0,!0)):!0}},_mouseMove:function(e){if(this._mouseMoved){if(t.ui.ie&&(!document.documentMode||9>document.documentMode)&&!e.button)return this._mouseUp(e);if(!e.which)if(e.originalEvent.altKey||e.originalEvent.ctrlKey||e.originalEvent.metaKey||e.originalEvent.shiftKey)this.ignoreMissingWhich=!0;else if(!this.ignoreMissingWhich)return this._mouseUp(e)}return(e.which||e.button)&&(this._mouseMoved=!0),this._mouseStarted?(this._mouseDrag(e),e.preventDefault()):(this._mouseDistanceMet(e)&&this._mouseDelayMet(e)&&(this._mouseStarted=this._mouseStart(this._mouseDownEvent,e)!==!1,this._mouseStarted?this._mouseDrag(e):this._mouseUp(e)),!this._mouseStarted)},_mouseUp:function(e){this.document.off("mousemove."+this.widgetName,this._mouseMoveDelegate).off("mouseup."+this.widgetName,this._mouseUpDelegate),this._mouseStarted&&(this._mouseStarted=!1,e.target===this._mouseDownEvent.target&&t.data(e.target,this.widgetName+".preventClickEvent",!0),this._mouseStop(e)),this._mouseDelayTimer&&(clearTimeout(this._mouseDelayTimer),delete this._mouseDelayTimer),this.ignoreMissingWhich=!1,_=!1,e.preventDefault()},_mouseDistanceMet:function(t){return Math.max(Math.abs(this._mouseDownEvent.pageX-t.pageX),Math.abs(this._mouseDownEvent.pageY-t.pageY))>=this.options.distance},_mouseDelayMet:function(){return this.mouseDelayMet},_mouseStart:function(){},_mouseDrag:function(){},_mouseStop:function(){},_mouseCapture:function(){return!0}}),t.ui.plugin={add:function(e,i,s){var n,o=t.ui[e].prototype;for(n in s)o.plugins[n]=o.plugins[n]||[],o.plugins[n].push([i,s[n]])},call:function(t,e,i,s){var n,o=t.plugins[e];if(o&&(s||t.element[0].parentNode&&11!==t.element[0].parentNode.nodeType))for(n=0;o.length>n;n++)t.options[o[n][0]]&&o[n][1].apply(t.element,i)}},t.ui.safeBlur=function(e){e&&"body"!==e.nodeName.toLowerCase()&&t(e).trigger("blur")},t.widget("ui.draggable",t.ui.mouse,{version:"1.12.0",widgetEventPrefix:"drag",options:{addClasses:!0,appendTo:"parent",axis:!1,connectToSortable:!1,containment:!1,cursor:"auto",cursorAt:!1,grid:!1,handle:!1,helper:"original",iframeFix:!1,opacity:!1,refreshPositions:!1,revert:!1,revertDuration:500,scope:"default",scroll:!0,scrollSensitivity:20,scrollSpeed:20,snap:!1,snapMode:"both",snapTolerance:20,stack:!1,zIndex:!1,drag:null,start:null,stop:null},_create:function(){"original"===this.options.helper&&this._setPositionRelative(),this.options.addClasses&&this._addClass("ui-draggable"),this._setHandleClassName(),this._mouseInit()},_setOption:function(t,e){this._super(t,e),"handle"===t&&(this._removeHandleClassName(),this._setHandleClassName())},_destroy:function(){return(this.helper||this.element).is(".ui-draggable-dragging")?(this.destroyOnClear=!0,void 0):(this._removeHandleClassName(),this._mouseDestroy(),void 0)},_mouseCapture:function(e){var i=this.options;return this._blurActiveElement(e),this.helper||i.disabled||t(e.target).closest(".ui-resizable-handle").length>0?!1:(this.handle=this._getHandle(e),this.handle?(this._blockFrames(i.iframeFix===!0?"iframe":i.iframeFix),!0):!1)},_blockFrames:function(e){this.iframeBlocks=this.document.find(e).map(function(){var e=t(this);return t("<div>").css("position","absolute").appendTo(e.parent()).outerWidth(e.outerWidth()).outerHeight(e.outerHeight()).offset(e.offset())[0]})},_unblockFrames:function(){this.iframeBlocks&&(this.iframeBlocks.remove(),delete this.iframeBlocks)},_blurActiveElement:function(e){var i=t.ui.safeActiveElement(this.document[0]),s=t(e.target);this._getHandle(e)&&s.closest(i).length||t.ui.safeBlur(i)},_mouseStart:function(e){var i=this.options;return this.helper=this._createHelper(e),this._addClass(this.helper,"ui-draggable-dragging"),this._cacheHelperProportions(),t.ui.ddmanager&&(t.ui.ddmanager.current=this),this._cacheMargins(),this.cssPosition=this.helper.css("position"),this.scrollParent=this.helper.scrollParent(!0),this.offsetParent=this.helper.offsetParent(),this.hasFixedAncestor=this.helper.parents().filter(function(){return"fixed"===t(this).css("position")}).length>0,this.positionAbs=this.element.offset(),this._refreshOffsets(e),this.originalPosition=this.position=this._generatePosition(e,!1),this.originalPageX=e.pageX,this.originalPageY=e.pageY,i.cursorAt&&this._adjustOffsetFromHelper(i.cursorAt),this._setContainment(),this._trigger("start",e)===!1?(this._clear(),!1):(this._cacheHelperProportions(),t.ui.ddmanager&&!i.dropBehaviour&&t.ui.ddmanager.prepareOffsets(this,e),this._mouseDrag(e,!0),t.ui.ddmanager&&t.ui.ddmanager.dragStart(this,e),!0)},_refreshOffsets:function(t){this.offset={top:this.positionAbs.top-this.margins.top,left:this.positionAbs.left-this.margins.left,scroll:!1,parent:this._getParentOffset(),relative:this._getRelativeOffset()},this.offset.click={left:t.pageX-this.offset.left,top:t.pageY-this.offset.top}},_mouseDrag:function(e,i){if(this.hasFixedAncestor&&(this.offset.parent=this._getParentOffset()),this.position=this._generatePosition(e,!0),this.positionAbs=this._convertPositionTo("absolute"),!i){var 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this._unblockFrames(),t.ui.ddmanager&&t.ui.ddmanager.dragStop(this,e),this.handleElement.is(e.target)&&this.element.trigger("focus"),t.ui.mouse.prototype._mouseUp.call(this,e)},cancel:function(){return this.helper.is(".ui-draggable-dragging")?this._mouseUp(new t.Event("mouseup",{target:this.element[0]})):this._clear(),this},_getHandle:function(e){return this.options.handle?!!t(e.target).closest(this.element.find(this.options.handle)).length:!0},_setHandleClassName:function(){this.handleElement=this.options.handle?this.element.find(this.options.handle):this.element,this._addClass(this.handleElement,"ui-draggable-handle")},_removeHandleClassName:function(){this._removeClass(this.handleElement,"ui-draggable-handle")},_createHelper:function(e){var i=this.options,s=t.isFunction(i.helper),n=s?t(i.helper.apply(this.element[0],[e])):"clone"===i.helper?this.element.clone().removeAttr("id"):this.element;return n.parents("body").length||n.appendTo("parent"===i.appendTo?this.element[0].parentNode:i.appendTo),s&&n[0]===this.element[0]&&this._setPositionRelative(),n[0]===this.element[0]||/(fixed|absolute)/.test(n.css("position"))||n.css("position","absolute"),n},_setPositionRelative:function(){/^(?:r|a|f)/.test(this.element.css("position"))||(this.element[0].style.position="relative")},_adjustOffsetFromHelper:function(e){"string"==typeof e&&(e=e.split(" ")),t.isArray(e)&&(e={left:+e[0],top:+e[1]||0}),"left"in e&&(this.offset.click.left=e.left+this.margins.left),"right"in e&&(this.offset.click.left=this.helperProportions.width-e.right+this.margins.left),"top"in e&&(this.offset.click.top=e.top+this.margins.top),"bottom"in e&&(this.offset.click.top=this.helperProportions.height-e.bottom+this.margins.top)},_isRootNode:function(t){return/(html|body)/i.test(t.tagName)||t===this.document[0]},_getParentOffset:function(){var 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+ | },_cacheMargins:function(){this.margins={left:parseInt(this.element.css("marginLeft"),10)||0,top:parseInt(this.element.css("marginTop"),10)||0,right:parseInt(this.element.css("marginRight"),10)||0,bottom:parseInt(this.element.css("marginBottom"),10)||0}},_cacheHelperProportions:function(){this.helperProportions={width:this.helper.outerWidth(),height:this.helper.outerHeight()}},_setContainment:function(){var e,i,s,n=this.options,o=this.document[0];return this.relativeContainer=null,n.containment?"window"===n.containment?(this.containment=[t(window).scrollLeft()-this.offset.relative.left-this.offset.parent.left,t(window).scrollTop()-this.offset.relative.top-this.offset.parent.top,t(window).scrollLeft()+t(window).width()-this.helperProportions.width-this.margins.left,t(window).scrollTop()+(t(window).height()||o.body.parentNode.scrollHeight)-this.helperProportions.height-this.margins.top],void 0):"document"===n.containment?(this.containment=[0,0,t(o).width()-this.helperProportions.width-this.margins.left,(t(o).height()||o.body.parentNode.scrollHeight)-this.helperProportions.height-this.margins.top],void 0):n.containment.constructor===Array?(this.containment=n.containment,void 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i="absolute"===t?1:-1,s=this._isRootNode(this.scrollParent[0]);return{top:e.top+this.offset.relative.top*i+this.offset.parent.top*i-("fixed"===this.cssPosition?-this.offset.scroll.top:s?0:this.offset.scroll.top)*i,left:e.left+this.offset.relative.left*i+this.offset.parent.left*i-("fixed"===this.cssPosition?-this.offset.scroll.left:s?0:this.offset.scroll.left)*i}},_generatePosition:function(t,e){var i,s,n,o,a=this.options,r=this._isRootNode(this.scrollParent[0]),h=t.pageX,l=t.pageY;return r&&this.offset.scroll||(this.offset.scroll={top:this.scrollParent.scrollTop(),left:this.scrollParent.scrollLeft()}),e&&(this.containment&&(this.relativeContainer?(s=this.relativeContainer.offset(),i=[this.containment[0]+s.left,this.containment[1]+s.top,this.containment[2]+s.left,this.containment[3]+s.top]):i=this.containment,t.pageX-this.offset.click.left<i[0]&&(h=i[0]+this.offset.click.left),t.pageY-this.offset.click.top<i[1]&&(l=i[1]+this.offset.click.top),t.pageX-this.offset.click.left>i[2]&&(h=i[2]+this.offset.click.left),t.pageY-this.offset.click.top>i[3]&&(l=i[3]+this.offset.click.top)),a.grid&&(n=a.grid[1]?this.originalPageY+Math.round((l-this.originalPageY)/a.grid[1])*a.grid[1]:this.originalPageY,l=i?n-this.offset.click.top>=i[1]||n-this.offset.click.top>i[3]?n:n-this.offset.click.top>=i[1]?n-a.grid[1]:n+a.grid[1]:n,o=a.grid[0]?this.originalPageX+Math.round((h-this.originalPageX)/a.grid[0])*a.grid[0]:this.originalPageX,h=i?o-this.offset.click.left>=i[0]||o-this.offset.click.left>i[2]?o:o-this.offset.click.left>=i[0]?o-a.grid[0]:o+a.grid[0]:o),"y"===a.axis&&(h=this.originalPageX),"x"===a.axis&&(l=this.originalPageY)),{top:l-this.offset.click.top-this.offset.relative.top-this.offset.parent.top+("fixed"===this.cssPosition?-this.offset.scroll.top:r?0:this.offset.scroll.top),left:h-this.offset.click.left-this.offset.relative.left-this.offset.parent.left+("fixed"===this.cssPosition?-this.offset.scroll.left:r?0:this.offset.scroll.left)}},_clear:function(){this._removeClass(this.helper,"ui-draggable-dragging"),this.helper[0]===this.element[0]||this.cancelHelperRemoval||this.helper.remove(),this.helper=null,this.cancelHelperRemoval=!1,this.destroyOnClear&&this.destroy()},_trigger:function(e,i,s){return s=s||this._uiHash(),t.ui.plugin.call(this,e,[i,s,this],!0),/^(drag|start|stop)/.test(e)&&(this.positionAbs=this._convertPositionTo("absolute"),s.offset=this.positionAbs),t.Widget.prototype._trigger.call(this,e,i,s)},plugins:{},_uiHash:function(){return{helper:this.helper,position:this.position,originalPosition:this.originalPosition,offset:this.positionAbs}}}),t.ui.plugin.add("draggable","connectToSortable",{start:function(e,i,s){var n=t.extend({},i,{item:s.element});s.sortables=[],t(s.options.connectToSortable).each(function(){var i=t(this).sortable("instance");i&&!i.options.disabled&&(s.sortables.push(i),i.refreshPositions(),i._trigger("activate",e,n))})},stop:function(e,i,s){var n=t.extend({},i,{item:s.element});s.cancelHelperRemoval=!1,t.each(s.sortables,function(){var t=this;t.isOver?(t.isOver=0,s.cancelHelperRemoval=!0,t.cancelHelperRemoval=!1,t._storedCSS={position:t.placeholder.css("position"),top:t.placeholder.css("top"),left:t.placeholder.css("left")},t._mouseStop(e),t.options.helper=t.options._helper):(t.cancelHelperRemoval=!0,t._trigger("deactivate",e,n))})},drag:function(e,i,s){t.each(s.sortables,function(){var n=!1,o=this;o.positionAbs=s.positionAbs,o.helperProportions=s.helperProportions,o.offset.click=s.offset.click,o._intersectsWith(o.containerCache)&&(n=!0,t.each(s.sortables,function(){return this.positionAbs=s.positionAbs,this.helperProportions=s.helperProportions,this.offset.click=s.offset.click,this!==o&&this._intersectsWith(this.containerCache)&&t.contains(o.element[0],this.element[0])&&(n=!1),n})),n?(o.isOver||(o.isOver=1,s._parent=i.helper.parent(),o.currentItem=i.helper.appendTo(o.element).data("ui-sortable-item",!0),o.options._helper=o.options.helper,o.options.helper=function(){return i.helper[0]},e.target=o.currentItem[0],o._mouseCapture(e,!0),o._mouseStart(e,!0,!0),o.offset.click.top=s.offset.click.top,o.offset.click.left=s.offset.click.left,o.offset.parent.left-=s.offset.parent.left-o.offset.parent.left,o.offset.parent.top-=s.offset.parent.top-o.offset.parent.top,s._trigger("toSortable",e),s.dropped=o.element,t.each(s.sortables,function(){this.refreshPositions()}),s.currentItem=s.element,o.fromOutside=s),o.currentItem&&(o._mouseDrag(e),i.position=o.position)):o.isOver&&(o.isOver=0,o.cancelHelperRemoval=!0,o.options._revert=o.options.revert,o.options.revert=!1,o._trigger("out",e,o._uiHash(o)),o._mouseStop(e,!0),o.options.revert=o.options._revert,o.options.helper=o.options._helper,o.placeholder&&o.placeholder.remove(),i.helper.appendTo(s._parent),s._refreshOffsets(e),i.position=s._generatePosition(e,!0),s._trigger("fromSortable",e),s.dropped=!1,t.each(s.sortables,function(){this.refreshPositions()}))})}}),t.ui.plugin.add("draggable","cursor",{start:function(e,i,s){var n=t("body"),o=s.options;n.css("cursor")&&(o._cursor=n.css("cursor")),n.css("cursor",o.cursor)},stop:function(e,i,s){var n=s.options;n._cursor&&t("body").css("cursor",n._cursor)}}),t.ui.plugin.add("draggable","opacity",{start:function(e,i,s){var n=t(i.helper),o=s.options;n.css("opacity")&&(o._opacity=n.css("opacity")),n.css("opacity",o.opacity)},stop:function(e,i,s){var n=s.options;n._opacity&&t(i.helper).css("opacity",n._opacity)}}),t.ui.plugin.add("draggable","scroll",{start:function(t,e,i){i.scrollParentNotHidden||(i.scrollParentNotHidden=i.helper.scrollParent(!1)),i.scrollParentNotHidden[0]!==i.document[0]&&"HTML"!==i.scrollParentNotHidden[0].tagName&&(i.overflowOffset=i.scrollParentNotHidden.offset())},drag:function(e,i,s){var n=s.options,o=!1,a=s.scrollParentNotHidden[0],r=s.document[0];a!==r&&"HTML"!==a.tagName?(n.axis&&"x"===n.axis||(s.overflowOffset.top+a.offsetHeight-e.pageY<n.scrollSensitivity?a.scrollTop=o=a.scrollTop+n.scrollSpeed:e.pageY-s.overflowOffset.top<n.scrollSensitivity&&(a.scrollTop=o=a.scrollTop-n.scrollSpeed)),n.axis&&"y"===n.axis||(s.overflowOffset.left+a.offsetWidth-e.pageX<n.scrollSensitivity?a.scrollLeft=o=a.scrollLeft+n.scrollSpeed:e.pageX-s.overflowOffset.left<n.scrollSensitivity&&(a.scrollLeft=o=a.scrollLeft-n.scrollSpeed))):(n.axis&&"x"===n.axis||(e.pageY-t(r).scrollTop()<n.scrollSensitivity?o=t(r).scrollTop(t(r).scrollTop()-n.scrollSpeed):t(window).height()-(e.pageY-t(r).scrollTop())<n.scrollSensitivity&&(o=t(r).scrollTop(t(r).scrollTop()+n.scrollSpeed))),n.axis&&"y"===n.axis||(e.pageX-t(r).scrollLeft()<n.scrollSensitivity?o=t(r).scrollLeft(t(r).scrollLeft()-n.scrollSpeed):t(window).width()-(e.pageX-t(r).scrollLeft())<n.scrollSensitivity&&(o=t(r).scrollLeft(t(r).scrollLeft()+n.scrollSpeed)))),o!==!1&&t.ui.ddmanager&&!n.dropBehaviour&&t.ui.ddmanager.prepareOffsets(s,e)}}),t.ui.plugin.add("draggable","snap",{start:function(e,i,s){var n=s.options;s.snapElements=[],t(n.snap.constructor!==String?n.snap.items||":data(ui-draggable)":n.snap).each(function(){var e=t(this),i=e.offset();this!==s.element[0]&&s.snapElements.push({item:this,width:e.outerWidth(),height:e.outerHeight(),top:i.top,left:i.left})})},drag:function(e,i,s){var 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n,o=s.options,a=t.makeArray(t(o.stack)).sort(function(e,i){return(parseInt(t(e).css("zIndex"),10)||0)-(parseInt(t(i).css("zIndex"),10)||0)});a.length&&(n=parseInt(t(a[0]).css("zIndex"),10)||0,t(a).each(function(e){t(this).css("zIndex",n+e)}),this.css("zIndex",n+a.length))}}),t.ui.plugin.add("draggable","zIndex",{start:function(e,i,s){var n=t(i.helper),o=s.options;n.css("zIndex")&&(o._zIndex=n.css("zIndex")),n.css("zIndex",o.zIndex)},stop:function(e,i,s){var n=s.options;n._zIndex&&t(i.helper).css("zIndex",n._zIndex)}}),t.ui.draggable,t.widget("ui.resizable",t.ui.mouse,{version:"1.12.0",widgetEventPrefix:"resize",options:{alsoResize:!1,animate:!1,animateDuration:"slow",animateEasing:"swing",aspectRatio:!1,autoHide:!1,classes:{"ui-resizable-se":"ui-icon ui-icon-gripsmall-diagonal-se"},containment:!1,ghost:!1,grid:!1,handles:"e,s,se",helper:!1,maxHeight:null,maxWidth:null,minHeight:10,minWidth:10,zIndex:90,resize:null,start:null,stop:null},_num:function(t){return 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i=t(this).resizable("instance"),s=i.options,n=i._proportionallyResizeElements,o=n.length&&/textarea/i.test(n[0].nodeName),a=o&&i._hasScroll(n[0],"left")?0:i.sizeDiff.height,r=o?0:i.sizeDiff.width,h={width:i.size.width-r,height:i.size.height-a},l=parseFloat(i.element.css("left"))+(i.position.left-i.originalPosition.left)||null,c=parseFloat(i.element.css("top"))+(i.position.top-i.originalPosition.top)||null;i.element.animate(t.extend(h,c&&l?{top:c,left:l}:{}),{duration:s.animateDuration,easing:s.animateEasing,step:function(){var s={width:parseFloat(i.element.css("width")),height:parseFloat(i.element.css("height")),top:parseFloat(i.element.css("top")),left:parseFloat(i.element.css("left"))};n&&n.length&&t(n[0]).css({width:s.width,height:s.height}),i._updateCache(s),i._propagate("resize",e)}})}}),t.ui.plugin.add("resizable","containment",{start:function(){var e,i,s,n,o,a,r,h=t(this).resizable("instance"),l=h.options,c=h.element,u=l.containment,d=u instanceof 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e=this.options.appendTo;return e&&(e.jquery||e.nodeType)?t(e):this.document.find(e||"body").eq(0)},_destroy:function(){var t,e=this.originalPosition;this._untrackInstance(),this._destroyOverlay(),this.element.removeUniqueId().css(this.originalCss).detach(),this.uiDialog.remove(),this.originalTitle&&this.element.attr("title",this.originalTitle),t=e.parent.children().eq(e.index),t.length&&t[0]!==this.element[0]?t.before(this.element):e.parent.append(this.element)},widget:function(){return this.uiDialog | ||
+ | },disable:t.noop,enable:t.noop,close:function(e){var i=this;this._isOpen&&this._trigger("beforeClose",e)!==!1&&(this._isOpen=!1,this._focusedElement=null,this._destroyOverlay(),this._untrackInstance(),this.opener.filter(":focusable").trigger("focus").length||t.ui.safeBlur(t.ui.safeActiveElement(this.document[0])),this._hide(this.uiDialog,this.options.hide,function(){i._trigger("close",e)}))},isOpen:function(){return this._isOpen},moveToTop:function(){this._moveToTop()},_moveToTop:function(e,i){var s=!1,n=this.uiDialog.siblings(".ui-front:visible").map(function(){return+t(this).css("z-index")}).get(),o=Math.max.apply(null,n);return o>=+this.uiDialog.css("z-index")&&(this.uiDialog.css("z-index",o+1),s=!0),s&&!i&&this._trigger("focus",e),s},open:function(){var e=this;return this._isOpen?(this._moveToTop()&&this._focusTabbable(),void 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e=t.ui.safeActiveElement(this.document[0]),i=this.uiDialog[0]===e||t.contains(this.uiDialog[0],e);i||this._focusTabbable()}e.preventDefault(),i.call(this),this._delay(i)},_createWrapper:function(){this.uiDialog=t("<div>").hide().attr({tabIndex:-1,role:"dialog"}).appendTo(this._appendTo()),this._addClass(this.uiDialog,"ui-dialog","ui-widget ui-widget-content ui-front"),this._on(this.uiDialog,{keydown:function(e){if(this.options.closeOnEscape&&!e.isDefaultPrevented()&&e.keyCode&&e.keyCode===t.ui.keyCode.ESCAPE)return e.preventDefault(),this.close(e),void 0;if(e.keyCode===t.ui.keyCode.TAB&&!e.isDefaultPrevented()){var i=this.uiDialog.find(":tabbable"),s=i.filter(":first"),n=i.filter(":last");e.target!==n[0]&&e.target!==this.uiDialog[0]||e.shiftKey?e.target!==s[0]&&e.target!==this.uiDialog[0]||!e.shiftKey||(this._delay(function(){n.trigger("focus")}),e.preventDefault()):(this._delay(function(){s.trigger("focus")}),e.preventDefault())}},mousedown:function(t){this._moveToTop(t)&&this._focusTabbable()}}),this.element.find("[aria-describedby]").length||this.uiDialog.attr({"aria-describedby":this.element.uniqueId().attr("id")})},_createTitlebar:function(){var e;this.uiDialogTitlebar=t("<div>"),this._addClass(this.uiDialogTitlebar,"ui-dialog-titlebar","ui-widget-header ui-helper-clearfix"),this._on(this.uiDialogTitlebar,{mousedown:function(e){t(e.target).closest(".ui-dialog-titlebar-close")||this.uiDialog.trigger("focus")}}),this.uiDialogTitlebarClose=t("<button 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t,e,i,s=this.options;this.element.show().css({width:"auto",minHeight:0,maxHeight:"none",height:0}),s.minWidth>s.width&&(s.width=s.minWidth),t=this.uiDialog.css({height:"auto",width:s.width}).outerHeight(),e=Math.max(0,s.minHeight-t),i="number"==typeof s.maxHeight?Math.max(0,s.maxHeight-t):"none","auto"===s.height?this.element.css({minHeight:e,maxHeight:i,height:"auto"}):this.element.height(Math.max(0,s.height-t)),this.uiDialog.is(":data(ui-resizable)")&&this.uiDialog.resizable("option","minHeight",this._minHeight())},_blockFrames:function(){this.iframeBlocks=this.document.find("iframe").map(function(){var e=t(this);return t("<div>").css({position:"absolute",width:e.outerWidth(),height:e.outerHeight()}).appendTo(e.parent()).offset(e.offset())[0]})},_unblockFrames:function(){this.iframeBlocks&&(this.iframeBlocks.remove(),delete this.iframeBlocks)},_allowInteraction:function(e){return t(e.target).closest(".ui-dialog").length?!0:!!t(e.target).closest(".ui-datepicker").length},_createOverlay:function(){if(this.options.modal){var e=!0;this._delay(function(){e=!1}),this.document.data("ui-dialog-overlays")||this._on(this.document,{focusin:function(t){e||this._allowInteraction(t)||(t.preventDefault(),this._trackingInstances()[0]._focusTabbable())}}),this.overlay=t("<div>").appendTo(this._appendTo()),this._addClass(this.overlay,null,"ui-widget-overlay ui-front"),this._on(this.overlay,{mousedown:"_keepFocus"}),this.document.data("ui-dialog-overlays",(this.document.data("ui-dialog-overlays")||0)+1)}},_destroyOverlay:function(){if(this.options.modal&&this.overlay){var t=this.document.data("ui-dialog-overlays")-1;t?this.document.data("ui-dialog-overlays",t):(this._off(this.document,"focusin"),this.document.removeData("ui-dialog-overlays")),this.overlay.remove(),this.overlay=null}}}),t.uiBackCompat!==!1&&t.widget("ui.dialog",t.ui.dialog,{options:{dialogClass:""},_createWrapper:function(){this._super(),this.uiDialog.addClass(this.options.dialogClass)},_setOption:function(t,e){"dialogClass"===t&&this.uiDialog.removeClass(this.options.dialogClass).addClass(e),this._superApply(arguments)}}),t.ui.dialog,t.widget("ui.droppable",{version:"1.12.0",widgetEventPrefix:"drop",options:{accept:"*",addClasses:!0,greedy:!1,scope:"default",tolerance:"intersect",activate:null,deactivate:null,drop:null,out:null,over:null},_create:function(){var e,i=this.options,s=i.accept;this.isover=!1,this.isout=!0,this.accept=t.isFunction(s)?s:function(t){return t.is(s)},this.proportions=function(){return arguments.length?(e=arguments[0],void 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i=t.ui.ddmanager.current;i&&(i.currentItem||i.element)[0]!==this.element[0]&&this.accept.call(this.element[0],i.currentItem||i.element)&&(this._addHoverClass(),this._trigger("over",e,this.ui(i)))},_out:function(e){var i=t.ui.ddmanager.current;i&&(i.currentItem||i.element)[0]!==this.element[0]&&this.accept.call(this.element[0],i.currentItem||i.element)&&(this._removeHoverClass(),this._trigger("out",e,this.ui(i)))},_drop:function(e,i){var s=i||t.ui.ddmanager.current,n=!1;return s&&(s.currentItem||s.element)[0]!==this.element[0]?(this.element.find(":data(ui-droppable)").not(".ui-draggable-dragging").each(function(){var i=t(this).droppable("instance");return i.options.greedy&&!i.options.disabled&&i.options.scope===s.options.scope&&i.accept.call(i.element[0],s.currentItem||s.element)&&v(s,t.extend(i,{offset:i.element.offset()}),i.options.tolerance,e)?(n=!0,!1):void 0}),n?!1:this.accept.call(this.element[0],s.currentItem||s.element)?(this._removeActiveClass(),this._removeHoverClass(),this._trigger("drop",e,this.ui(s)),this.element):!1):!1},ui:function(t){return{draggable:t.currentItem||t.element,helper:t.helper,position:t.position,offset:t.positionAbs}},_addHoverClass:function(){this._addClass("ui-droppable-hover")},_removeHoverClass:function(){this._removeClass("ui-droppable-hover")},_addActiveClass:function(){this._addClass("ui-droppable-active")},_removeActiveClass:function(){this._removeClass("ui-droppable-active")}});var v=t.ui.intersect=function(){function t(t,e,i){return t>=e&&e+i>t}return function(e,i,s,n){if(!i.offset)return!1;var o=(e.positionAbs||e.position.absolute).left+e.margins.left,a=(e.positionAbs||e.position.absolute).top+e.margins.top,r=o+e.helperProportions.width,h=a+e.helperProportions.height,l=i.offset.left,c=i.offset.top,u=l+i.proportions().width,d=c+i.proportions().height;switch(s){case"fit":return o>=l&&u>=r&&a>=c&&d>=h;case"intersect":return o+e.helperProportions.width/2>l&&u>r-e.helperProportions.width/2&&a+e.helperProportions.height/2>c&&d>h-e.helperProportions.height/2;case"pointer":return t(n.pageY,c,i.proportions().height)&&t(n.pageX,l,i.proportions().width);case"touch":return(a>=c&&d>=a||h>=c&&d>=h||c>a&&h>d)&&(o>=l&&u>=o||r>=l&&u>=r||l>o&&r>u);default:return!1}}}();t.ui.ddmanager={current:null,droppables:{"default":[]},prepareOffsets:function(e,i){var s,n,o=t.ui.ddmanager.droppables[e.options.scope]||[],a=i?i.type:null,r=(e.currentItem||e.element).find(":data(ui-droppable)").addBack();t:for(s=0;o.length>s;s++)if(!(o[s].options.disabled||e&&!o[s].accept.call(o[s].element[0],e.currentItem||e.element))){for(n=0;r.length>n;n++)if(r[n]===o[s].element[0]){o[s].proportions().height=0;continue t}o[s].visible="none"!==o[s].element.css("display"),o[s].visible&&("mousedown"===a&&o[s]._activate.call(o[s],i),o[s].offset=o[s].element.offset(),o[s].proportions({width:o[s].element[0].offsetWidth,height:o[s].element[0].offsetHeight}))}},drop:function(e,i){var s=!1;return t.each((t.ui.ddmanager.droppables[e.options.scope]||[]).slice(),function(){this.options&&(!this.options.disabled&&this.visible&&v(e,this,this.options.tolerance,i)&&(s=this._drop.call(this,i)||s),!this.options.disabled&&this.visible&&this.accept.call(this.element[0],e.currentItem||e.element)&&(this.isout=!0,this.isover=!1,this._deactivate.call(this,i)))}),s},dragStart:function(e,i){e.element.parentsUntil("body").on("scroll.droppable",function(){e.options.refreshPositions||t.ui.ddmanager.prepareOffsets(e,i)})},drag:function(e,i){e.options.refreshPositions&&t.ui.ddmanager.prepareOffsets(e,i),t.each(t.ui.ddmanager.droppables[e.options.scope]||[],function(){if(!this.options.disabled&&!this.greedyChild&&this.visible){var s,n,o,a=v(e,this,this.options.tolerance,i),r=!a&&this.isover?"isout":a&&!this.isover?"isover":null;r&&(this.options.greedy&&(n=this.options.scope,o=this.element.parents(":data(ui-droppable)").filter(function(){return t(this).droppable("instance").options.scope===n}),o.length&&(s=t(o[0]).droppable("instance"),s.greedyChild="isover"===r)),s&&"isover"===r&&(s.isover=!1,s.isout=!0,s._out.call(s,i)),this[r]=!0,this["isout"===r?"isover":"isout"]=!1,this["isover"===r?"_over":"_out"].call(this,i),s&&"isout"===r&&(s.isout=!1,s.isover=!0,s._over.call(s,i)))}})},dragStop:function(e,i){e.element.parentsUntil("body").off("scroll.droppable"),e.options.refreshPositions||t.ui.ddmanager.prepareOffsets(e,i)}},t.uiBackCompat!==!1&&t.widget("ui.droppable",t.ui.droppable,{options:{hoverClass:!1,activeClass:!1},_addActiveClass:function(){this._super(),this.options.activeClass&&this.element.addClass(this.options.activeClass)},_removeActiveClass:function(){this._super(),this.options.activeClass&&this.element.removeClass(this.options.activeClass)},_addHoverClass:function(){this._super(),this.options.hoverClass&&this.element.addClass(this.options.hoverClass)},_removeHoverClass:function(){this._super(),this.options.hoverClass&&this.element.removeClass(this.options.hoverClass)}}),t.ui.droppable,t.widget("ui.progressbar",{version:"1.12.0",options:{classes:{"ui-progressbar":"ui-corner-all","ui-progressbar-value":"ui-corner-left","ui-progressbar-complete":"ui-corner-right"},max:100,value:0,change:null,complete:null},min:0,_create:function(){this.oldValue=this.options.value=this._constrainedValue(),this.element.attr({role:"progressbar","aria-valuemin":this.min}),this._addClass("ui-progressbar","ui-widget ui-widget-content"),this.valueDiv=t("<div>").appendTo(this.element),this._addClass(this.valueDiv,"ui-progressbar-value","ui-widget-header"),this._refreshValue()},_destroy:function(){this.element.removeAttr("role aria-valuemin aria-valuemax aria-valuenow"),this.valueDiv.remove()},value:function(t){return void 0===t?this.options.value:(this.options.value=this._constrainedValue(t),this._refreshValue(),void 0)},_constrainedValue:function(t){return void 0===t&&(t=this.options.value),this.indeterminate=t===!1,"number"!=typeof t&&(t=0),this.indeterminate?!1:Math.min(this.options.max,Math.max(this.min,t))},_setOptions:function(t){var e=t.value;delete t.value,this._super(t),this.options.value=this._constrainedValue(e),this._refreshValue()},_setOption:function(t,e){"max"===t&&(e=Math.max(this.min,e)),this._super(t,e)},_setOptionDisabled:function(t){this._super(t),this.element.attr("aria-disabled",t),this._toggleClass(null,"ui-state-disabled",!!t)},_percentage:function(){return this.indeterminate?100:100*(this.options.value-this.min)/(this.options.max-this.min)},_refreshValue:function(){var e=this.options.value,i=this._percentage();this.valueDiv.toggle(this.indeterminate||e>this.min).width(i.toFixed(0)+"%"),this._toggleClass(this.valueDiv,"ui-progressbar-complete",null,e===this.options.max)._toggleClass("ui-progressbar-indeterminate",null,this.indeterminate),this.indeterminate?(this.element.removeAttr("aria-valuenow"),this.overlayDiv||(this.overlayDiv=t("<div>").appendTo(this.valueDiv),this._addClass(this.overlayDiv,"ui-progressbar-overlay"))):(this.element.attr({"aria-valuemax":this.options.max,"aria-valuenow":e}),this.overlayDiv&&(this.overlayDiv.remove(),this.overlayDiv=null)),this.oldValue!==e&&(this.oldValue=e,this._trigger("change")),e===this.options.max&&this._trigger("complete")}}),t.widget("ui.selectable",t.ui.mouse,{version:"1.12.0",options:{appendTo:"body",autoRefresh:!0,distance:0,filter:"*",tolerance:"touch",selected:null,selecting:null,start:null,stop:null,unselected:null,unselecting:null},_create:function(){var e=this;this._addClass("ui-selectable"),this.dragged=!1,this.refresh=function(){e.elementPos=t(e.element[0]).offset(),e.selectees=t(e.options.filter,e.element[0]),e._addClass(e.selectees,"ui-selectee"),e.selectees.each(function(){var i=t(this),s=i.offset(),n={left:s.left-e.elementPos.left,top:s.top-e.elementPos.top};t.data(this,"selectable-item",{element:this,$element:i,left:n.left,top:n.top,right:n.left+i.outerWidth(),bottom:n.top+i.outerHeight(),startselected:!1,selected:i.hasClass("ui-selected"),selecting:i.hasClass("ui-selecting"),unselecting:i.hasClass("ui-unselecting")})})},this.refresh(),this._mouseInit(),this.helper=t("<div>"),this._addClass(this.helper,"ui-selectable-helper")},_destroy:function(){this.selectees.removeData("selectable-item"),this._mouseDestroy()},_mouseStart:function(e){var i=this,s=this.options;this.opos=[e.pageX,e.pageY],this.elementPos=t(this.element[0]).offset(),this.options.disabled||(this.selectees=t(s.filter,this.element[0]),this._trigger("start",e),t(s.appendTo).append(this.helper),this.helper.css({left:e.pageX,top:e.pageY,width:0,height:0}),s.autoRefresh&&this.refresh(),this.selectees.filter(".ui-selected").each(function(){var s=t.data(this,"selectable-item");s.startselected=!0,e.metaKey||e.ctrlKey||(i._removeClass(s.$element,"ui-selected"),s.selected=!1,i._addClass(s.$element,"ui-unselecting"),s.unselecting=!0,i._trigger("unselecting",e,{unselecting:s.element}))}),t(e.target).parents().addBack().each(function(){var s,n=t.data(this,"selectable-item");return n?(s=!e.metaKey&&!e.ctrlKey||!n.$element.hasClass("ui-selected"),i._removeClass(n.$element,s?"ui-unselecting":"ui-selected")._addClass(n.$element,s?"ui-selecting":"ui-unselecting"),n.unselecting=!s,n.selecting=s,n.selected=s,s?i._trigger("selecting",e,{selecting:n.element}):i._trigger("unselecting",e,{unselecting:n.element}),!1):void 0}))},_mouseDrag:function(e){if(this.dragged=!0,!this.options.disabled){var i,s=this,n=this.options,o=this.opos[0],a=this.opos[1],r=e.pageX,h=e.pageY;return o>r&&(i=r,r=o,o=i),a>h&&(i=h,h=a,a=i),this.helper.css({left:o,top:a,width:r-o,height:h-a}),this.selectees.each(function(){var i=t.data(this,"selectable-item"),l=!1,c={};i&&i.element!==s.element[0]&&(c.left=i.left+s.elementPos.left,c.right=i.right+s.elementPos.left,c.top=i.top+s.elementPos.top,c.bottom=i.bottom+s.elementPos.top,"touch"===n.tolerance?l=!(c.left>r||o>c.right||c.top>h||a>c.bottom):"fit"===n.tolerance&&(l=c.left>o&&r>c.right&&c.top>a&&h>c.bottom),l?(i.selected&&(s._removeClass(i.$element,"ui-selected"),i.selected=!1),i.unselecting&&(s._removeClass(i.$element,"ui-unselecting"),i.unselecting=!1),i.selecting||(s._addClass(i.$element,"ui-selecting"),i.selecting=!0,s._trigger("selecting",e,{selecting:i.element}))):(i.selecting&&((e.metaKey||e.ctrlKey)&&i.startselected?(s._removeClass(i.$element,"ui-selecting"),i.selecting=!1,s._addClass(i.$element,"ui-selected"),i.selected=!0):(s._removeClass(i.$element,"ui-selecting"),i.selecting=!1,i.startselected&&(s._addClass(i.$element,"ui-unselecting"),i.unselecting=!0),s._trigger("unselecting",e,{unselecting:i.element}))),i.selected&&(e.metaKey||e.ctrlKey||i.startselected||(s._removeClass(i.$element,"ui-selected"),i.selected=!1,s._addClass(i.$element,"ui-unselecting"),i.unselecting=!0,s._trigger("unselecting",e,{unselecting:i.element})))))}),!1}},_mouseStop:function(e){var i=this;return this.dragged=!1,t(".ui-unselecting",this.element[0]).each(function(){var s=t.data(this,"selectable-item");i._removeClass(s.$element,"ui-unselecting"),s.unselecting=!1,s.startselected=!1,i._trigger("unselected",e,{unselected:s.element})}),t(".ui-selecting",this.element[0]).each(function(){var s=t.data(this,"selectable-item");i._removeClass(s.$element,"ui-selecting")._addClass(s.$element,"ui-selected"),s.selecting=!1,s.selected=!0,s.startselected=!0,i._trigger("selected",e,{selected:s.element})}),this._trigger("stop",e),this.helper.remove(),!1}}),t.widget("ui.selectmenu",[t.ui.formResetMixin,{version:"1.12.0",defaultElement:"<select>",options:{appendTo:null,classes:{"ui-selectmenu-button-open":"ui-corner-top","ui-selectmenu-button-closed":"ui-corner-all"},disabled:null,icons:{button:"ui-icon-triangle-1-s"},position:{my:"left top",at:"left bottom",collision:"none"},width:!1,change:null,close:null,focus:null,open:null,select:null},_create:function(){var e=this.element.uniqueId().attr("id");this.ids={element:e,button:e+"-button",menu:e+"-menu"},this._drawButton(),this._drawMenu(),this._bindFormResetHandler(),this._rendered=!1,this.menuItems=t()},_drawButton:function(){var e,i=this,s=this._parseOption(this.element.find("option:selected"),this.element[0].selectedIndex);this.labels=this.element.labels().attr("for",this.ids.button),this._on(this.labels,{click:function(t){this.button.focus(),t.preventDefault()}}),this.element.hide(),this.button=t("<span>",{tabindex:this.options.disabled?-1:0,id:this.ids.button,role:"combobox","aria-expanded":"false","aria-autocomplete":"list","aria-owns":this.ids.menu,"aria-haspopup":"true",title:this.element.attr("title")}).insertAfter(this.element),this._addClass(this.button,"ui-selectmenu-button ui-selectmenu-button-closed","ui-button ui-widget"),e=t("<span>").appendTo(this.button),this._addClass(e,"ui-selectmenu-icon","ui-icon "+this.options.icons.button),this.buttonItem=this._renderButtonItem(s).appendTo(this.button),this.options.width!==!1&&this._resizeButton(),this._on(this.button,this._buttonEvents),this.button.one("focusin",function(){i._rendered||i._refreshMenu()})},_drawMenu:function(){var e=this;this.menu=t("<ul>",{"aria-hidden":"true","aria-labelledby":this.ids.button,id:this.ids.menu}),this.menuWrap=t("<div>").append(this.menu),this._addClass(this.menuWrap,"ui-selectmenu-menu","ui-front"),this.menuWrap.appendTo(this._appendTo()),this.menuInstance=this.menu.menu({classes:{"ui-menu":"ui-corner-bottom"},role:"listbox",select:function(t,i){t.preventDefault(),e._setSelection(),e._select(i.item.data("ui-selectmenu-item"),t)},focus:function(t,i){var s=i.item.data("ui-selectmenu-item");null!=e.focusIndex&&s.index!==e.focusIndex&&(e._trigger("focus",t,{item:s}),e.isOpen||e._select(s,t)),e.focusIndex=s.index,e.button.attr("aria-activedescendant",e.menuItems.eq(s.index).attr("id"))}}).menu("instance"),this.menuInstance._off(this.menu,"mouseleave"),this.menuInstance._closeOnDocumentClick=function(){return!1},this.menuInstance._isDivider=function(){return!1}},refresh:function(){this._refreshMenu(),this.buttonItem.replaceWith(this.buttonItem=this._renderButtonItem(this._getSelectedItem().data("ui-selectmenu-item")||{})),null===this.options.width&&this._resizeButton()},_refreshMenu:function(){var t,e=this.element.find("option");this.menu.empty(),this._parseOptions(e),this._renderMenu(this.menu,this.items),this.menuInstance.refresh(),this.menuItems=this.menu.find("li").not(".ui-selectmenu-optgroup").find(".ui-menu-item-wrapper"),this._rendered=!0,e.length&&(t=this._getSelectedItem(),this.menuInstance.focus(null,t),this._setAria(t.data("ui-selectmenu-item")),this._setOption("disabled",this.element.prop("disabled")))},open:function(t){this.options.disabled||(this._rendered?(this._removeClass(this.menu.find(".ui-state-active"),null,"ui-state-active"),this.menuInstance.focus(null,this._getSelectedItem())):this._refreshMenu(),this.menuItems.length&&(this.isOpen=!0,this._toggleAttr(),this._resizeMenu(),this._position(),this._on(this.document,this._documentClick),this._trigger("open",t)))},_position:function(){this.menuWrap.position(t.extend({of:this.button},this.options.position))},close:function(t){this.isOpen&&(this.isOpen=!1,this._toggleAttr(),this.range=null,this._off(this.document),this._trigger("close",t))},widget:function(){return this.button},menuWidget:function(){return this.menu},_renderButtonItem:function(e){var i=t("<span>");return this._setText(i,e.label),this._addClass(i,"ui-selectmenu-text"),i},_renderMenu:function(e,i){var s=this,n="";t.each(i,function(i,o){var a;o.optgroup!==n&&(a=t("<li>",{text:o.optgroup}),s._addClass(a,"ui-selectmenu-optgroup","ui-menu-divider"+(o.element.parent("optgroup").prop("disabled")?" ui-state-disabled":"")),a.appendTo(e),n=o.optgroup),s._renderItemData(e,o)})},_renderItemData:function(t,e){return this._renderItem(t,e).data("ui-selectmenu-item",e)},_renderItem:function(e,i){var s=t("<li>"),n=t("<div>",{title:i.element.attr("title")});return i.disabled&&this._addClass(s,null,"ui-state-disabled"),this._setText(n,i.label),s.append(n).appendTo(e)},_setText:function(t,e){e?t.text(e):t.html(" ")},_move:function(t,e){var i,s,n=".ui-menu-item";this.isOpen?i=this.menuItems.eq(this.focusIndex).parent("li"):(i=this.menuItems.eq(this.element[0].selectedIndex).parent("li"),n+=":not(.ui-state-disabled)"),s="first"===t||"last"===t?i["first"===t?"prevAll":"nextAll"](n).eq(-1):i[t+"All"](n).eq(0),s.length&&this.menuInstance.focus(e,s)},_getSelectedItem:function(){return this.menuItems.eq(this.element[0].selectedIndex).parent("li")},_toggle:function(t){this[this.isOpen?"close":"open"](t)},_setSelection:function(){var t;this.range&&(window.getSelection?(t=window.getSelection(),t.removeAllRanges(),t.addRange(this.range)):this.range.select(),this.button.focus())},_documentClick:{mousedown:function(e){this.isOpen&&(t(e.target).closest(".ui-selectmenu-menu, #"+t.ui.escapeSelector(this.ids.button)).length||this.close(e))}},_buttonEvents:{mousedown:function(){var t;window.getSelection?(t=window.getSelection(),t.rangeCount&&(this.range=t.getRangeAt(0))):this.range=document.selection.createRange()},click:function(t){this._setSelection(),this._toggle(t)},keydown:function(e){var i=!0;switch(e.keyCode){case t.ui.keyCode.TAB:case t.ui.keyCode.ESCAPE:this.close(e),i=!1;break;case t.ui.keyCode.ENTER:this.isOpen&&this._selectFocusedItem(e);break;case t.ui.keyCode.UP:e.altKey?this._toggle(e):this._move("prev",e);break;case t.ui.keyCode.DOWN:e.altKey?this._toggle(e):this._move("next",e);break;case t.ui.keyCode.SPACE:this.isOpen?this._selectFocusedItem(e):this._toggle(e);break;case t.ui.keyCode.LEFT:this._move("prev",e);break;case t.ui.keyCode.RIGHT:this._move("next",e);break;case t.ui.keyCode.HOME:case t.ui.keyCode.PAGE_UP:this._move("first",e);break;case t.ui.keyCode.END:case t.ui.keyCode.PAGE_DOWN:this._move("last",e);break;default:this.menu.trigger(e),i=!1}i&&e.preventDefault()}},_selectFocusedItem:function(t){var e=this.menuItems.eq(this.focusIndex).parent("li");e.hasClass("ui-state-disabled")||this._select(e.data("ui-selectmenu-item"),t)},_select:function(t,e){var i=this.element[0].selectedIndex;this.element[0].selectedIndex=t.index,this.buttonItem.replaceWith(this.buttonItem=this._renderButtonItem(t)),this._setAria(t),this._trigger("select",e,{item:t}),t.index!==i&&this._trigger("change",e,{item:t}),this.close(e)},_setAria:function(t){var e=this.menuItems.eq(t.index).attr("id");this.button.attr({"aria-labelledby":e,"aria-activedescendant":e}),this.menu.attr("aria-activedescendant",e)},_setOption:function(t,e){if("icons"===t){var i=this.button.find("span.ui-icon");this._removeClass(i,null,this.options.icons.button)._addClass(i,null,e.button)}this._super(t,e),"appendTo"===t&&this.menuWrap.appendTo(this._appendTo()),"width"===t&&this._resizeButton()},_setOptionDisabled:function(t){this._super(t),this.menuInstance.option("disabled",t),this.button.attr("aria-disabled",t),this._toggleClass(this.button,null,"ui-state-disabled",t),this.element.prop("disabled",t),t?(this.button.attr("tabindex",-1),this.close()):this.button.attr("tabindex",0)},_appendTo:function(){var e=this.options.appendTo;return e&&(e=e.jquery||e.nodeType?t(e):this.document.find(e).eq(0)),e&&e[0]||(e=this.element.closest(".ui-front, dialog")),e.length||(e=this.document[0].body),e},_toggleAttr:function(){this.button.attr("aria-expanded",this.isOpen),this._removeClass(this.button,"ui-selectmenu-button-"+(this.isOpen?"closed":"open"))._addClass(this.button,"ui-selectmenu-button-"+(this.isOpen?"open":"closed"))._toggleClass(this.menuWrap,"ui-selectmenu-open",null,this.isOpen),this.menu.attr("aria-hidden",!this.isOpen)},_resizeButton:function(){var t=this.options.width;return t===!1?(this.button.css("width",""),void 0):(null===t&&(t=this.element.show().outerWidth(),this.element.hide()),this.button.outerWidth(t),void 0)},_resizeMenu:function(){this.menu.outerWidth(Math.max(this.button.outerWidth(),this.menu.width("").outerWidth()+1))},_getCreateOptions:function(){var t=this._super();return t.disabled=this.element.prop("disabled"),t},_parseOptions:function(e){var i=this,s=[];e.each(function(e,n){s.push(i._parseOption(t(n),e))}),this.items=s},_parseOption:function(t,e){var i=t.parent("optgroup");return{element:t,index:e,value:t.val(),label:t.text(),optgroup:i.attr("label")||"",disabled:i.prop("disabled")||t.prop("disabled")}},_destroy:function(){this._unbindFormResetHandler(),this.menuWrap.remove(),this.button.remove(),this.element.show(),this.element.removeUniqueId(),this.labels.attr("for",this.ids.element)}}]),t.widget("ui.slider",t.ui.mouse,{version:"1.12.0",widgetEventPrefix:"slide",options:{animate:!1,classes:{"ui-slider":"ui-corner-all","ui-slider-handle":"ui-corner-all","ui-slider-range":"ui-corner-all ui-widget-header"},distance:0,max:100,min:0,orientation:"horizontal",range:!1,step:1,value:0,values:null,change:null,slide:null,start:null,stop:null},numPages:5,_create:function(){this._keySliding=!1,this._mouseSliding=!1,this._animateOff=!0,this._handleIndex=null,this._detectOrientation(),this._mouseInit(),this._calculateNewMax(),this._addClass("ui-slider ui-slider-"+this.orientation,"ui-widget ui-widget-content"),this._refresh(),this._animateOff=!1 | ||
+ | },_refresh:function(){this._createRange(),this._createHandles(),this._setupEvents(),this._refreshValue()},_createHandles:function(){var e,i,s=this.options,n=this.element.find(".ui-slider-handle"),o="<span tabindex='0'></span>",a=[];for(i=s.values&&s.values.length||1,n.length>i&&(n.slice(i).remove(),n=n.slice(0,i)),e=n.length;i>e;e++)a.push(o);this.handles=n.add(t(a.join("")).appendTo(this.element)),this._addClass(this.handles,"ui-slider-handle","ui-state-default"),this.handle=this.handles.eq(0),this.handles.each(function(e){t(this).data("ui-slider-handle-index",e)})},_createRange:function(){var e=this.options;e.range?(e.range===!0&&(e.values?e.values.length&&2!==e.values.length?e.values=[e.values[0],e.values[0]]:t.isArray(e.values)&&(e.values=e.values.slice(0)):e.values=[this._valueMin(),this._valueMin()]),this.range&&this.range.length?(this._removeClass(this.range,"ui-slider-range-min ui-slider-range-max"),this.range.css({left:"",bottom:""})):(this.range=t("<div>").appendTo(this.element),this._addClass(this.range,"ui-slider-range")),("min"===e.range||"max"===e.range)&&this._addClass(this.range,"ui-slider-range-"+e.range)):(this.range&&this.range.remove(),this.range=null)},_setupEvents:function(){this._off(this.handles),this._on(this.handles,this._handleEvents),this._hoverable(this.handles),this._focusable(this.handles)},_destroy:function(){this.handles.remove(),this.range&&this.range.remove(),this._mouseDestroy()},_mouseCapture:function(e){var i,s,n,o,a,r,h,l,c=this,u=this.options;return u.disabled?!1:(this.elementSize={width:this.element.outerWidth(),height:this.element.outerHeight()},this.elementOffset=this.element.offset(),i={x:e.pageX,y:e.pageY},s=this._normValueFromMouse(i),n=this._valueMax()-this._valueMin()+1,this.handles.each(function(e){var i=Math.abs(s-c.values(e));(n>i||n===i&&(e===c._lastChangedValue||c.values(e)===u.min))&&(n=i,o=t(this),a=e)}),r=this._start(e,a),r===!1?!1:(this._mouseSliding=!0,this._handleIndex=a,this._addClass(o,null,"ui-state-active"),o.trigger("focus"),h=o.offset(),l=!t(e.target).parents().addBack().is(".ui-slider-handle"),this._clickOffset=l?{left:0,top:0}:{left:e.pageX-h.left-o.width()/2,top:e.pageY-h.top-o.height()/2-(parseInt(o.css("borderTopWidth"),10)||0)-(parseInt(o.css("borderBottomWidth"),10)||0)+(parseInt(o.css("marginTop"),10)||0)},this.handles.hasClass("ui-state-hover")||this._slide(e,a,s),this._animateOff=!0,!0))},_mouseStart:function(){return!0},_mouseDrag:function(t){var e={x:t.pageX,y:t.pageY},i=this._normValueFromMouse(e);return this._slide(t,this._handleIndex,i),!1},_mouseStop:function(t){return this._removeClass(this.handles,null,"ui-state-active"),this._mouseSliding=!1,this._stop(t,this._handleIndex),this._change(t,this._handleIndex),this._handleIndex=null,this._clickOffset=null,this._animateOff=!1,!1},_detectOrientation:function(){this.orientation="vertical"===this.options.orientation?"vertical":"horizontal"},_normValueFromMouse:function(t){var e,i,s,n,o;return"horizontal"===this.orientation?(e=this.elementSize.width,i=t.x-this.elementOffset.left-(this._clickOffset?this._clickOffset.left:0)):(e=this.elementSize.height,i=t.y-this.elementOffset.top-(this._clickOffset?this._clickOffset.top:0)),s=i/e,s>1&&(s=1),0>s&&(s=0),"vertical"===this.orientation&&(s=1-s),n=this._valueMax()-this._valueMin(),o=this._valueMin()+s*n,this._trimAlignValue(o)},_uiHash:function(t,e,i){var s={handle:this.handles[t],handleIndex:t,value:void 0!==e?e:this.value()};return this._hasMultipleValues()&&(s.value=void 0!==e?e:this.values(t),s.values=i||this.values()),s},_hasMultipleValues:function(){return this.options.values&&this.options.values.length},_start:function(t,e){return this._trigger("start",t,this._uiHash(e))},_slide:function(t,e,i){var s,n,o=this.value(),a=this.values();this._hasMultipleValues()&&(n=this.values(e?0:1),o=this.values(e),2===this.options.values.length&&this.options.range===!0&&(i=0===e?Math.min(n,i):Math.max(n,i)),a[e]=i),i!==o&&(s=this._trigger("slide",t,this._uiHash(e,i,a)),s!==!1&&(this._hasMultipleValues()?this.values(e,i):this.value(i)))},_stop:function(t,e){this._trigger("stop",t,this._uiHash(e))},_change:function(t,e){this._keySliding||this._mouseSliding||(this._lastChangedValue=e,this._trigger("change",t,this._uiHash(e)))},value:function(t){return arguments.length?(this.options.value=this._trimAlignValue(t),this._refreshValue(),this._change(null,0),void 0):this._value()},values:function(e,i){var s,n,o;if(arguments.length>1)return this.options.values[e]=this._trimAlignValue(i),this._refreshValue(),this._change(null,e),void 0;if(!arguments.length)return this._values();if(!t.isArray(arguments[0]))return this._hasMultipleValues()?this._values(e):this.value();for(s=this.options.values,n=arguments[0],o=0;s.length>o;o+=1)s[o]=this._trimAlignValue(n[o]),this._change(null,o);this._refreshValue()},_setOption:function(e,i){var s,n=0;switch("range"===e&&this.options.range===!0&&("min"===i?(this.options.value=this._values(0),this.options.values=null):"max"===i&&(this.options.value=this._values(this.options.values.length-1),this.options.values=null)),t.isArray(this.options.values)&&(n=this.options.values.length),this._super(e,i),e){case"orientation":this._detectOrientation(),this._removeClass("ui-slider-horizontal ui-slider-vertical")._addClass("ui-slider-"+this.orientation),this._refreshValue(),this.options.range&&this._refreshRange(i),this.handles.css("horizontal"===i?"bottom":"left","");break;case"value":this._animateOff=!0,this._refreshValue(),this._change(null,0),this._animateOff=!1;break;case"values":for(this._animateOff=!0,this._refreshValue(),s=n-1;s>=0;s--)this._change(null,s);this._animateOff=!1;break;case"step":case"min":case"max":this._animateOff=!0,this._calculateNewMax(),this._refreshValue(),this._animateOff=!1;break;case"range":this._animateOff=!0,this._refresh(),this._animateOff=!1}},_setOptionDisabled:function(t){this._super(t),this._toggleClass(null,"ui-state-disabled",!!t)},_value:function(){var t=this.options.value;return t=this._trimAlignValue(t)},_values:function(t){var e,i,s;if(arguments.length)return e=this.options.values[t],e=this._trimAlignValue(e);if(this._hasMultipleValues()){for(i=this.options.values.slice(),s=0;i.length>s;s+=1)i[s]=this._trimAlignValue(i[s]);return i}return[]},_trimAlignValue:function(t){if(this._valueMin()>=t)return this._valueMin();if(t>=this._valueMax())return this._valueMax();var e=this.options.step>0?this.options.step:1,i=(t-this._valueMin())%e,s=t-i;return 2*Math.abs(i)>=e&&(s+=i>0?e:-e),parseFloat(s.toFixed(5))},_calculateNewMax:function(){var t=this.options.max,e=this._valueMin(),i=this.options.step,s=Math.round((t-e)/i)*i;t=s+e,t>this.options.max&&(t-=i),this.max=parseFloat(t.toFixed(this._precision()))},_precision:function(){var t=this._precisionOf(this.options.step);return null!==this.options.min&&(t=Math.max(t,this._precisionOf(this.options.min))),t},_precisionOf:function(t){var e=""+t,i=e.indexOf(".");return-1===i?0:e.length-i-1},_valueMin:function(){return this.options.min},_valueMax:function(){return this.max},_refreshRange:function(t){"vertical"===t&&this.range.css({width:"",left:""}),"horizontal"===t&&this.range.css({height:"",bottom:""})},_refreshValue:function(){var e,i,s,n,o,a=this.options.range,r=this.options,h=this,l=this._animateOff?!1:r.animate,c={};this._hasMultipleValues()?this.handles.each(function(s){i=100*((h.values(s)-h._valueMin())/(h._valueMax()-h._valueMin())),c["horizontal"===h.orientation?"left":"bottom"]=i+"%",t(this).stop(1,1)[l?"animate":"css"](c,r.animate),h.options.range===!0&&("horizontal"===h.orientation?(0===s&&h.range.stop(1,1)[l?"animate":"css"]({left:i+"%"},r.animate),1===s&&h.range[l?"animate":"css"]({width:i-e+"%"},{queue:!1,duration:r.animate})):(0===s&&h.range.stop(1,1)[l?"animate":"css"]({bottom:i+"%"},r.animate),1===s&&h.range[l?"animate":"css"]({height:i-e+"%"},{queue:!1,duration:r.animate}))),e=i}):(s=this.value(),n=this._valueMin(),o=this._valueMax(),i=o!==n?100*((s-n)/(o-n)):0,c["horizontal"===this.orientation?"left":"bottom"]=i+"%",this.handle.stop(1,1)[l?"animate":"css"](c,r.animate),"min"===a&&"horizontal"===this.orientation&&this.range.stop(1,1)[l?"animate":"css"]({width:i+"%"},r.animate),"max"===a&&"horizontal"===this.orientation&&this.range.stop(1,1)[l?"animate":"css"]({width:100-i+"%"},r.animate),"min"===a&&"vertical"===this.orientation&&this.range.stop(1,1)[l?"animate":"css"]({height:i+"%"},r.animate),"max"===a&&"vertical"===this.orientation&&this.range.stop(1,1)[l?"animate":"css"]({height:100-i+"%"},r.animate))},_handleEvents:{keydown:function(e){var i,s,n,o,a=t(e.target).data("ui-slider-handle-index");switch(e.keyCode){case t.ui.keyCode.HOME:case t.ui.keyCode.END:case t.ui.keyCode.PAGE_UP:case t.ui.keyCode.PAGE_DOWN:case t.ui.keyCode.UP:case t.ui.keyCode.RIGHT:case t.ui.keyCode.DOWN:case t.ui.keyCode.LEFT:if(e.preventDefault(),!this._keySliding&&(this._keySliding=!0,this._addClass(t(e.target),null,"ui-state-active"),i=this._start(e,a),i===!1))return}switch(o=this.options.step,s=n=this._hasMultipleValues()?this.values(a):this.value(),e.keyCode){case t.ui.keyCode.HOME:n=this._valueMin();break;case t.ui.keyCode.END:n=this._valueMax();break;case t.ui.keyCode.PAGE_UP:n=this._trimAlignValue(s+(this._valueMax()-this._valueMin())/this.numPages);break;case t.ui.keyCode.PAGE_DOWN:n=this._trimAlignValue(s-(this._valueMax()-this._valueMin())/this.numPages);break;case t.ui.keyCode.UP:case t.ui.keyCode.RIGHT:if(s===this._valueMax())return;n=this._trimAlignValue(s+o);break;case t.ui.keyCode.DOWN:case t.ui.keyCode.LEFT:if(s===this._valueMin())return;n=this._trimAlignValue(s-o)}this._slide(e,a,n)},keyup:function(e){var i=t(e.target).data("ui-slider-handle-index");this._keySliding&&(this._keySliding=!1,this._stop(e,i),this._change(e,i),this._removeClass(t(e.target),null,"ui-state-active"))}}}),t.widget("ui.sortable",t.ui.mouse,{version:"1.12.0",widgetEventPrefix:"sort",ready:!1,options:{appendTo:"parent",axis:!1,connectWith:!1,containment:!1,cursor:"auto",cursorAt:!1,dropOnEmpty:!0,forcePlaceholderSize:!1,forceHelperSize:!1,grid:!1,handle:!1,helper:"original",items:"> *",opacity:!1,placeholder:!1,revert:!1,scroll:!0,scrollSensitivity:20,scrollSpeed:20,scope:"default",tolerance:"intersect",zIndex:1e3,activate:null,beforeStop:null,change:null,deactivate:null,out:null,over:null,receive:null,remove:null,sort:null,start:null,stop:null,update:null},_isOverAxis:function(t,e,i){return t>=e&&e+i>t},_isFloating:function(t){return/left|right/.test(t.css("float"))||/inline|table-cell/.test(t.css("display"))},_create:function(){this.containerCache={},this._addClass("ui-sortable"),this.refresh(),this.offset=this.element.offset(),this._mouseInit(),this._setHandleClassName(),this.ready=!0},_setOption:function(t,e){this._super(t,e),"handle"===t&&this._setHandleClassName()},_setHandleClassName:function(){var e=this;this._removeClass(this.element.find(".ui-sortable-handle"),"ui-sortable-handle"),t.each(this.items,function(){e._addClass(this.instance.options.handle?this.item.find(this.instance.options.handle):this.item,"ui-sortable-handle")})},_destroy:function(){this._mouseDestroy();for(var t=this.items.length-1;t>=0;t--)this.items[t].item.removeData(this.widgetName+"-item");return this},_mouseCapture:function(e,i){var s=null,n=!1,o=this;return this.reverting?!1:this.options.disabled||"static"===this.options.type?!1:(this._refreshItems(e),t(e.target).parents().each(function(){return t.data(this,o.widgetName+"-item")===o?(s=t(this),!1):void 0}),t.data(e.target,o.widgetName+"-item")===o&&(s=t(e.target)),s?!this.options.handle||i||(t(this.options.handle,s).find("*").addBack().each(function(){this===e.target&&(n=!0)}),n)?(this.currentItem=s,this._removeCurrentsFromItems(),!0):!1:!1)},_mouseStart:function(e,i,s){var n,o,a=this.options;if(this.currentContainer=this,this.refreshPositions(),this.helper=this._createHelper(e),this._cacheHelperProportions(),this._cacheMargins(),this.scrollParent=this.helper.scrollParent(),this.offset=this.currentItem.offset(),this.offset={top:this.offset.top-this.margins.top,left:this.offset.left-this.margins.left},t.extend(this.offset,{click:{left:e.pageX-this.offset.left,top:e.pageY-this.offset.top},parent:this._getParentOffset(),relative:this._getRelativeOffset()}),this.helper.css("position","absolute"),this.cssPosition=this.helper.css("position"),this.originalPosition=this._generatePosition(e),this.originalPageX=e.pageX,this.originalPageY=e.pageY,a.cursorAt&&this._adjustOffsetFromHelper(a.cursorAt),this.domPosition={prev:this.currentItem.prev()[0],parent:this.currentItem.parent()[0]},this.helper[0]!==this.currentItem[0]&&this.currentItem.hide(),this._createPlaceholder(),a.containment&&this._setContainment(),a.cursor&&"auto"!==a.cursor&&(o=this.document.find("body"),this.storedCursor=o.css("cursor"),o.css("cursor",a.cursor),this.storedStylesheet=t("<style>*{ cursor: "+a.cursor+" !important; }</style>").appendTo(o)),a.opacity&&(this.helper.css("opacity")&&(this._storedOpacity=this.helper.css("opacity")),this.helper.css("opacity",a.opacity)),a.zIndex&&(this.helper.css("zIndex")&&(this._storedZIndex=this.helper.css("zIndex")),this.helper.css("zIndex",a.zIndex)),this.scrollParent[0]!==this.document[0]&&"HTML"!==this.scrollParent[0].tagName&&(this.overflowOffset=this.scrollParent.offset()),this._trigger("start",e,this._uiHash()),this._preserveHelperProportions||this._cacheHelperProportions(),!s)for(n=this.containers.length-1;n>=0;n--)this.containers[n]._trigger("activate",e,this._uiHash(this));return t.ui.ddmanager&&(t.ui.ddmanager.current=this),t.ui.ddmanager&&!a.dropBehaviour&&t.ui.ddmanager.prepareOffsets(this,e),this.dragging=!0,this._addClass(this.helper,"ui-sortable-helper"),this._mouseDrag(e),!0},_mouseDrag:function(e){var i,s,n,o,a=this.options,r=!1;for(this.position=this._generatePosition(e),this.positionAbs=this._convertPositionTo("absolute"),this.lastPositionAbs||(this.lastPositionAbs=this.positionAbs),this.options.scroll&&(this.scrollParent[0]!==this.document[0]&&"HTML"!==this.scrollParent[0].tagName?(this.overflowOffset.top+this.scrollParent[0].offsetHeight-e.pageY<a.scrollSensitivity?this.scrollParent[0].scrollTop=r=this.scrollParent[0].scrollTop+a.scrollSpeed:e.pageY-this.overflowOffset.top<a.scrollSensitivity&&(this.scrollParent[0].scrollTop=r=this.scrollParent[0].scrollTop-a.scrollSpeed),this.overflowOffset.left+this.scrollParent[0].offsetWidth-e.pageX<a.scrollSensitivity?this.scrollParent[0].scrollLeft=r=this.scrollParent[0].scrollLeft+a.scrollSpeed:e.pageX-this.overflowOffset.left<a.scrollSensitivity&&(this.scrollParent[0].scrollLeft=r=this.scrollParent[0].scrollLeft-a.scrollSpeed)):(e.pageY-this.document.scrollTop()<a.scrollSensitivity?r=this.document.scrollTop(this.document.scrollTop()-a.scrollSpeed):this.window.height()-(e.pageY-this.document.scrollTop())<a.scrollSensitivity&&(r=this.document.scrollTop(this.document.scrollTop()+a.scrollSpeed)),e.pageX-this.document.scrollLeft()<a.scrollSensitivity?r=this.document.scrollLeft(this.document.scrollLeft()-a.scrollSpeed):this.window.width()-(e.pageX-this.document.scrollLeft())<a.scrollSensitivity&&(r=this.document.scrollLeft(this.document.scrollLeft()+a.scrollSpeed))),r!==!1&&t.ui.ddmanager&&!a.dropBehaviour&&t.ui.ddmanager.prepareOffsets(this,e)),this.positionAbs=this._convertPositionTo("absolute"),this.options.axis&&"y"===this.options.axis||(this.helper[0].style.left=this.position.left+"px"),this.options.axis&&"x"===this.options.axis||(this.helper[0].style.top=this.position.top+"px"),i=this.items.length-1;i>=0;i--)if(s=this.items[i],n=s.item[0],o=this._intersectsWithPointer(s),o&&s.instance===this.currentContainer&&n!==this.currentItem[0]&&this.placeholder[1===o?"next":"prev"]()[0]!==n&&!t.contains(this.placeholder[0],n)&&("semi-dynamic"===this.options.type?!t.contains(this.element[0],n):!0)){if(this.direction=1===o?"down":"up","pointer"!==this.options.tolerance&&!this._intersectsWithSides(s))break;this._rearrange(e,s),this._trigger("change",e,this._uiHash());break}return this._contactContainers(e),t.ui.ddmanager&&t.ui.ddmanager.drag(this,e),this._trigger("sort",e,this._uiHash()),this.lastPositionAbs=this.positionAbs,!1},_mouseStop:function(e,i){if(e){if(t.ui.ddmanager&&!this.options.dropBehaviour&&t.ui.ddmanager.drop(this,e),this.options.revert){var s=this,n=this.placeholder.offset(),o=this.options.axis,a={};o&&"x"!==o||(a.left=n.left-this.offset.parent.left-this.margins.left+(this.offsetParent[0]===this.document[0].body?0:this.offsetParent[0].scrollLeft)),o&&"y"!==o||(a.top=n.top-this.offset.parent.top-this.margins.top+(this.offsetParent[0]===this.document[0].body?0:this.offsetParent[0].scrollTop)),this.reverting=!0,t(this.helper).animate(a,parseInt(this.options.revert,10)||500,function(){s._clear(e)})}else this._clear(e,i);return!1}},cancel:function(){if(this.dragging){this._mouseUp({target:null}),"original"===this.options.helper?(this.currentItem.css(this._storedCSS),this._removeClass(this.currentItem,"ui-sortable-helper")):this.currentItem.show();for(var e=this.containers.length-1;e>=0;e--)this.containers[e]._trigger("deactivate",null,this._uiHash(this)),this.containers[e].containerCache.over&&(this.containers[e]._trigger("out",null,this._uiHash(this)),this.containers[e].containerCache.over=0)}return this.placeholder&&(this.placeholder[0].parentNode&&this.placeholder[0].parentNode.removeChild(this.placeholder[0]),"original"!==this.options.helper&&this.helper&&this.helper[0].parentNode&&this.helper.remove(),t.extend(this,{helper:null,dragging:!1,reverting:!1,_noFinalSort:null}),this.domPosition.prev?t(this.domPosition.prev).after(this.currentItem):t(this.domPosition.parent).prepend(this.currentItem)),this},serialize:function(e){var i=this._getItemsAsjQuery(e&&e.connected),s=[];return e=e||{},t(i).each(function(){var i=(t(e.item||this).attr(e.attribute||"id")||"").match(e.expression||/(.+)[\-=_](.+)/);i&&s.push((e.key||i[1]+"[]")+"="+(e.key&&e.expression?i[1]:i[2]))}),!s.length&&e.key&&s.push(e.key+"="),s.join("&")},toArray:function(e){var i=this._getItemsAsjQuery(e&&e.connected),s=[];return e=e||{},i.each(function(){s.push(t(e.item||this).attr(e.attribute||"id")||"")}),s},_intersectsWith:function(t){var e=this.positionAbs.left,i=e+this.helperProportions.width,s=this.positionAbs.top,n=s+this.helperProportions.height,o=t.left,a=o+t.width,r=t.top,h=r+t.height,l=this.offset.click.top,c=this.offset.click.left,u="x"===this.options.axis||s+l>r&&h>s+l,d="y"===this.options.axis||e+c>o&&a>e+c,p=u&&d;return"pointer"===this.options.tolerance||this.options.forcePointerForContainers||"pointer"!==this.options.tolerance&&this.helperProportions[this.floating?"width":"height"]>t[this.floating?"width":"height"]?p:e+this.helperProportions.width/2>o&&a>i-this.helperProportions.width/2&&s+this.helperProportions.height/2>r&&h>n-this.helperProportions.height/2},_intersectsWithPointer:function(t){var e,i,s="x"===this.options.axis||this._isOverAxis(this.positionAbs.top+this.offset.click.top,t.top,t.height),n="y"===this.options.axis||this._isOverAxis(this.positionAbs.left+this.offset.click.left,t.left,t.width),o=s&&n;return o?(e=this._getDragVerticalDirection(),i=this._getDragHorizontalDirection(),this.floating?"right"===i||"down"===e?2:1:e&&("down"===e?2:1)):!1},_intersectsWithSides:function(t){var e=this._isOverAxis(this.positionAbs.top+this.offset.click.top,t.top+t.height/2,t.height),i=this._isOverAxis(this.positionAbs.left+this.offset.click.left,t.left+t.width/2,t.width),s=this._getDragVerticalDirection(),n=this._getDragHorizontalDirection();return this.floating&&n?"right"===n&&i||"left"===n&&!i:s&&("down"===s&&e||"up"===s&&!e)},_getDragVerticalDirection:function(){var t=this.positionAbs.top-this.lastPositionAbs.top;return 0!==t&&(t>0?"down":"up")},_getDragHorizontalDirection:function(){var t=this.positionAbs.left-this.lastPositionAbs.left;return 0!==t&&(t>0?"right":"left")},refresh:function(t){return this._refreshItems(t),this._setHandleClassName(),this.refreshPositions(),this},_connectWith:function(){var t=this.options;return t.connectWith.constructor===String?[t.connectWith]:t.connectWith},_getItemsAsjQuery:function(e){function i(){r.push(this)}var s,n,o,a,r=[],h=[],l=this._connectWith();if(l&&e)for(s=l.length-1;s>=0;s--)for(o=t(l[s],this.document[0]),n=o.length-1;n>=0;n--)a=t.data(o[n],this.widgetFullName),a&&a!==this&&!a.options.disabled&&h.push([t.isFunction(a.options.items)?a.options.items.call(a.element):t(a.options.items,a.element).not(".ui-sortable-helper").not(".ui-sortable-placeholder"),a]);for(h.push([t.isFunction(this.options.items)?this.options.items.call(this.element,null,{options:this.options,item:this.currentItem}):t(this.options.items,this.element).not(".ui-sortable-helper").not(".ui-sortable-placeholder"),this]),s=h.length-1;s>=0;s--)h[s][0].each(i);return t(r)},_removeCurrentsFromItems:function(){var e=this.currentItem.find(":data("+this.widgetName+"-item)");this.items=t.grep(this.items,function(t){for(var i=0;e.length>i;i++)if(e[i]===t.item[0])return!1;return!0})},_refreshItems:function(e){this.items=[],this.containers=[this];var i,s,n,o,a,r,h,l,c=this.items,u=[[t.isFunction(this.options.items)?this.options.items.call(this.element[0],e,{item:this.currentItem}):t(this.options.items,this.element),this]],d=this._connectWith();if(d&&this.ready)for(i=d.length-1;i>=0;i--)for(n=t(d[i],this.document[0]),s=n.length-1;s>=0;s--)o=t.data(n[s],this.widgetFullName),o&&o!==this&&!o.options.disabled&&(u.push([t.isFunction(o.options.items)?o.options.items.call(o.element[0],e,{item:this.currentItem}):t(o.options.items,o.element),o]),this.containers.push(o));for(i=u.length-1;i>=0;i--)for(a=u[i][1],r=u[i][0],s=0,l=r.length;l>s;s++)h=t(r[s]),h.data(this.widgetName+"-item",a),c.push({item:h,instance:a,width:0,height:0,left:0,top:0})},refreshPositions:function(e){this.floating=this.items.length?"x"===this.options.axis||this._isFloating(this.items[0].item):!1,this.offsetParent&&this.helper&&(this.offset.parent=this._getParentOffset());var i,s,n,o;for(i=this.items.length-1;i>=0;i--)s=this.items[i],s.instance!==this.currentContainer&&this.currentContainer&&s.item[0]!==this.currentItem[0]||(n=this.options.toleranceElement?t(this.options.toleranceElement,s.item):s.item,e||(s.width=n.outerWidth(),s.height=n.outerHeight()),o=n.offset(),s.left=o.left,s.top=o.top);if(this.options.custom&&this.options.custom.refreshContainers)this.options.custom.refreshContainers.call(this);else for(i=this.containers.length-1;i>=0;i--)o=this.containers[i].element.offset(),this.containers[i].containerCache.left=o.left,this.containers[i].containerCache.top=o.top,this.containers[i].containerCache.width=this.containers[i].element.outerWidth(),this.containers[i].containerCache.height=this.containers[i].element.outerHeight();return this},_createPlaceholder:function(e){e=e||this;var i,s=e.options;s.placeholder&&s.placeholder.constructor!==String||(i=s.placeholder,s.placeholder={element:function(){var s=e.currentItem[0].nodeName.toLowerCase(),n=t("<"+s+">",e.document[0]);return e._addClass(n,"ui-sortable-placeholder",i||e.currentItem[0].className)._removeClass(n,"ui-sortable-helper"),"tbody"===s?e._createTrPlaceholder(e.currentItem.find("tr").eq(0),t("<tr>",e.document[0]).appendTo(n)):"tr"===s?e._createTrPlaceholder(e.currentItem,n):"img"===s&&n.attr("src",e.currentItem.attr("src")),i||n.css("visibility","hidden"),n},update:function(t,n){(!i||s.forcePlaceholderSize)&&(n.height()||n.height(e.currentItem.innerHeight()-parseInt(e.currentItem.css("paddingTop")||0,10)-parseInt(e.currentItem.css("paddingBottom")||0,10)),n.width()||n.width(e.currentItem.innerWidth()-parseInt(e.currentItem.css("paddingLeft")||0,10)-parseInt(e.currentItem.css("paddingRight")||0,10)))}}),e.placeholder=t(s.placeholder.element.call(e.element,e.currentItem)),e.currentItem.after(e.placeholder),s.placeholder.update(e,e.placeholder)},_createTrPlaceholder:function(e,i){var s=this;e.children().each(function(){t("<td> </td>",s.document[0]).attr("colspan",t(this).attr("colspan")||1).appendTo(i)})},_contactContainers:function(e){var i,s,n,o,a,r,h,l,c,u,d=null,p=null;for(i=this.containers.length-1;i>=0;i--)if(!t.contains(this.currentItem[0],this.containers[i].element[0]))if(this._intersectsWith(this.containers[i].containerCache)){if(d&&t.contains(this.containers[i].element[0],d.element[0]))continue;d=this.containers[i],p=i}else this.containers[i].containerCache.over&&(this.containers[i]._trigger("out",e,this._uiHash(this)),this.containers[i].containerCache.over=0);if(d)if(1===this.containers.length)this.containers[p].containerCache.over||(this.containers[p]._trigger("over",e,this._uiHash(this)),this.containers[p].containerCache.over=1);else{for(n=1e4,o=null,c=d.floating||this._isFloating(this.currentItem),a=c?"left":"top",r=c?"width":"height",u=c?"pageX":"pageY",s=this.items.length-1;s>=0;s--)t.contains(this.containers[p].element[0],this.items[s].item[0])&&this.items[s].item[0]!==this.currentItem[0]&&(h=this.items[s].item.offset()[a],l=!1,e[u]-h>this.items[s][r]/2&&(l=!0),n>Math.abs(e[u]-h)&&(n=Math.abs(e[u]-h),o=this.items[s],this.direction=l?"up":"down"));if(!o&&!this.options.dropOnEmpty)return;if(this.currentContainer===this.containers[p])return this.currentContainer.containerCache.over||(this.containers[p]._trigger("over",e,this._uiHash()),this.currentContainer.containerCache.over=1),void 0;o?this._rearrange(e,o,null,!0):this._rearrange(e,null,this.containers[p].element,!0),this._trigger("change",e,this._uiHash()),this.containers[p]._trigger("change",e,this._uiHash(this)),this.currentContainer=this.containers[p],this.options.placeholder.update(this.currentContainer,this.placeholder),this.containers[p]._trigger("over",e,this._uiHash(this)),this.containers[p].containerCache.over=1}},_createHelper:function(e){var i=this.options,s=t.isFunction(i.helper)?t(i.helper.apply(this.element[0],[e,this.currentItem])):"clone"===i.helper?this.currentItem.clone():this.currentItem;return s.parents("body").length||t("parent"!==i.appendTo?i.appendTo:this.currentItem[0].parentNode)[0].appendChild(s[0]),s[0]===this.currentItem[0]&&(this._storedCSS={width:this.currentItem[0].style.width,height:this.currentItem[0].style.height,position:this.currentItem.css("position"),top:this.currentItem.css("top"),left:this.currentItem.css("left")}),(!s[0].style.width||i.forceHelperSize)&&s.width(this.currentItem.width()),(!s[0].style.height||i.forceHelperSize)&&s.height(this.currentItem.height()),s},_adjustOffsetFromHelper:function(e){"string"==typeof e&&(e=e.split(" ")),t.isArray(e)&&(e={left:+e[0],top:+e[1]||0}),"left"in e&&(this.offset.click.left=e.left+this.margins.left),"right"in e&&(this.offset.click.left=this.helperProportions.width-e.right+this.margins.left),"top"in e&&(this.offset.click.top=e.top+this.margins.top),"bottom"in e&&(this.offset.click.top=this.helperProportions.height-e.bottom+this.margins.top)},_getParentOffset:function(){this.offsetParent=this.helper.offsetParent();var e=this.offsetParent.offset();return"absolute"===this.cssPosition&&this.scrollParent[0]!==this.document[0]&&t.contains(this.scrollParent[0],this.offsetParent[0])&&(e.left+=this.scrollParent.scrollLeft(),e.top+=this.scrollParent.scrollTop()),(this.offsetParent[0]===this.document[0].body||this.offsetParent[0].tagName&&"html"===this.offsetParent[0].tagName.toLowerCase()&&t.ui.ie)&&(e={top:0,left:0}),{top:e.top+(parseInt(this.offsetParent.css("borderTopWidth"),10)||0),left:e.left+(parseInt(this.offsetParent.css("borderLeftWidth"),10)||0)}},_getRelativeOffset:function(){if("relative"===this.cssPosition){var t=this.currentItem.position();return{top:t.top-(parseInt(this.helper.css("top"),10)||0)+this.scrollParent.scrollTop(),left:t.left-(parseInt(this.helper.css("left"),10)||0)+this.scrollParent.scrollLeft()}}return{top:0,left:0}},_cacheMargins:function(){this.margins={left:parseInt(this.currentItem.css("marginLeft"),10)||0,top:parseInt(this.currentItem.css("marginTop"),10)||0}},_cacheHelperProportions:function(){this.helperProportions={width:this.helper.outerWidth(),height:this.helper.outerHeight()}},_setContainment:function(){var e,i,s,n=this.options;"parent"===n.containment&&(n.containment=this.helper[0].parentNode),("document"===n.containment||"window"===n.containment)&&(this.containment=[0-this.offset.relative.left-this.offset.parent.left,0-this.offset.relative.top-this.offset.parent.top,"document"===n.containment?this.document.width():this.window.width()-this.helperProportions.width-this.margins.left,("document"===n.containment?this.document.height()||document.body.parentNode.scrollHeight:this.window.height()||this.document[0].body.parentNode.scrollHeight)-this.helperProportions.height-this.margins.top]),/^(document|window|parent)$/.test(n.containment)||(e=t(n.containment)[0],i=t(n.containment).offset(),s="hidden"!==t(e).css("overflow"),this.containment=[i.left+(parseInt(t(e).css("borderLeftWidth"),10)||0)+(parseInt(t(e).css("paddingLeft"),10)||0)-this.margins.left,i.top+(parseInt(t(e).css("borderTopWidth"),10)||0)+(parseInt(t(e).css("paddingTop"),10)||0)-this.margins.top,i.left+(s?Math.max(e.scrollWidth,e.offsetWidth):e.offsetWidth)-(parseInt(t(e).css("borderLeftWidth"),10)||0)-(parseInt(t(e).css("paddingRight"),10)||0)-this.helperProportions.width-this.margins.left,i.top+(s?Math.max(e.scrollHeight,e.offsetHeight):e.offsetHeight)-(parseInt(t(e).css("borderTopWidth"),10)||0)-(parseInt(t(e).css("paddingBottom"),10)||0)-this.helperProportions.height-this.margins.top])},_convertPositionTo:function(e,i){i||(i=this.position);var s="absolute"===e?1:-1,n="absolute"!==this.cssPosition||this.scrollParent[0]!==this.document[0]&&t.contains(this.scrollParent[0],this.offsetParent[0])?this.scrollParent:this.offsetParent,o=/(html|body)/i.test(n[0].tagName);return{top:i.top+this.offset.relative.top*s+this.offset.parent.top*s-("fixed"===this.cssPosition?-this.scrollParent.scrollTop():o?0:n.scrollTop())*s,left:i.left+this.offset.relative.left*s+this.offset.parent.left*s-("fixed"===this.cssPosition?-this.scrollParent.scrollLeft():o?0:n.scrollLeft())*s}},_generatePosition:function(e){var i,s,n=this.options,o=e.pageX,a=e.pageY,r="absolute"!==this.cssPosition||this.scrollParent[0]!==this.document[0]&&t.contains(this.scrollParent[0],this.offsetParent[0])?this.scrollParent:this.offsetParent,h=/(html|body)/i.test(r[0].tagName);return"relative"!==this.cssPosition||this.scrollParent[0]!==this.document[0]&&this.scrollParent[0]!==this.offsetParent[0]||(this.offset.relative=this._getRelativeOffset()),this.originalPosition&&(this.containment&&(e.pageX-this.offset.click.left<this.containment[0]&&(o=this.containment[0]+this.offset.click.left),e.pageY-this.offset.click.top<this.containment[1]&&(a=this.containment[1]+this.offset.click.top),e.pageX-this.offset.click.left>this.containment[2]&&(o=this.containment[2]+this.offset.click.left),e.pageY-this.offset.click.top>this.containment[3]&&(a=this.containment[3]+this.offset.click.top)),n.grid&&(i=this.originalPageY+Math.round((a-this.originalPageY)/n.grid[1])*n.grid[1],a=this.containment?i-this.offset.click.top>=this.containment[1]&&i-this.offset.click.top<=this.containment[3]?i:i-this.offset.click.top>=this.containment[1]?i-n.grid[1]:i+n.grid[1]:i,s=this.originalPageX+Math.round((o-this.originalPageX)/n.grid[0])*n.grid[0],o=this.containment?s-this.offset.click.left>=this.containment[0]&&s-this.offset.click.left<=this.containment[2]?s:s-this.offset.click.left>=this.containment[0]?s-n.grid[0]:s+n.grid[0]:s)),{top:a-this.offset.click.top-this.offset.relative.top-this.offset.parent.top+("fixed"===this.cssPosition?-this.scrollParent.scrollTop():h?0:r.scrollTop()),left:o-this.offset.click.left-this.offset.relative.left-this.offset.parent.left+("fixed"===this.cssPosition?-this.scrollParent.scrollLeft():h?0:r.scrollLeft())}},_rearrange:function(t,e,i,s){i?i[0].appendChild(this.placeholder[0]):e.item[0].parentNode.insertBefore(this.placeholder[0],"down"===this.direction?e.item[0]:e.item[0].nextSibling),this.counter=this.counter?++this.counter:1;var n=this.counter;this._delay(function(){n===this.counter&&this.refreshPositions(!s) | ||
+ | })},_clear:function(t,e){function i(t,e,i){return function(s){i._trigger(t,s,e._uiHash(e))}}this.reverting=!1;var s,n=[];if(!this._noFinalSort&&this.currentItem.parent().length&&this.placeholder.before(this.currentItem),this._noFinalSort=null,this.helper[0]===this.currentItem[0]){for(s in this._storedCSS)("auto"===this._storedCSS[s]||"static"===this._storedCSS[s])&&(this._storedCSS[s]="");this.currentItem.css(this._storedCSS),this._removeClass(this.currentItem,"ui-sortable-helper")}else this.currentItem.show();for(this.fromOutside&&!e&&n.push(function(t){this._trigger("receive",t,this._uiHash(this.fromOutside))}),!this.fromOutside&&this.domPosition.prev===this.currentItem.prev().not(".ui-sortable-helper")[0]&&this.domPosition.parent===this.currentItem.parent()[0]||e||n.push(function(t){this._trigger("update",t,this._uiHash())}),this!==this.currentContainer&&(e||(n.push(function(t){this._trigger("remove",t,this._uiHash())}),n.push(function(t){return function(e){t._trigger("receive",e,this._uiHash(this))}}.call(this,this.currentContainer)),n.push(function(t){return function(e){t._trigger("update",e,this._uiHash(this))}}.call(this,this.currentContainer)))),s=this.containers.length-1;s>=0;s--)e||n.push(i("deactivate",this,this.containers[s])),this.containers[s].containerCache.over&&(n.push(i("out",this,this.containers[s])),this.containers[s].containerCache.over=0);if(this.storedCursor&&(this.document.find("body").css("cursor",this.storedCursor),this.storedStylesheet.remove()),this._storedOpacity&&this.helper.css("opacity",this._storedOpacity),this._storedZIndex&&this.helper.css("zIndex","auto"===this._storedZIndex?"":this._storedZIndex),this.dragging=!1,e||this._trigger("beforeStop",t,this._uiHash()),this.placeholder[0].parentNode.removeChild(this.placeholder[0]),this.cancelHelperRemoval||(this.helper[0]!==this.currentItem[0]&&this.helper.remove(),this.helper=null),!e){for(s=0;n.length>s;s++)n[s].call(this,t);this._trigger("stop",t,this._uiHash())}return this.fromOutside=!1,!this.cancelHelperRemoval},_trigger:function(){t.Widget.prototype._trigger.apply(this,arguments)===!1&&this.cancel()},_uiHash:function(e){var i=e||this;return{helper:i.helper,placeholder:i.placeholder||t([]),position:i.position,originalPosition:i.originalPosition,offset:i.positionAbs,item:i.currentItem,sender:e?e.element:null}}}),t.widget("ui.spinner",{version:"1.12.0",defaultElement:"<input>",widgetEventPrefix:"spin",options:{classes:{"ui-spinner":"ui-corner-all","ui-spinner-down":"ui-corner-br","ui-spinner-up":"ui-corner-tr"},culture:null,icons:{down:"ui-icon-triangle-1-s",up:"ui-icon-triangle-1-n"},incremental:!0,max:null,min:null,numberFormat:null,page:10,step:1,change:null,spin:null,start:null,stop:null},_create:function(){this._setOption("max",this.options.max),this._setOption("min",this.options.min),this._setOption("step",this.options.step),""!==this.value()&&this._value(this.element.val(),!0),this._draw(),this._on(this._events),this._refresh(),this._on(this.window,{beforeunload:function(){this.element.removeAttr("autocomplete")}})},_getCreateOptions:function(){var e=this._super(),i=this.element;return t.each(["min","max","step"],function(t,s){var n=i.attr(s);null!=n&&n.length&&(e[s]=n)}),e},_events:{keydown:function(t){this._start(t)&&this._keydown(t)&&t.preventDefault()},keyup:"_stop",focus:function(){this.previous=this.element.val()},blur:function(t){return this.cancelBlur?(delete this.cancelBlur,void 0):(this._stop(),this._refresh(),this.previous!==this.element.val()&&this._trigger("change",t),void 0)},mousewheel:function(t,e){if(e){if(!this.spinning&&!this._start(t))return!1;this._spin((e>0?1:-1)*this.options.step,t),clearTimeout(this.mousewheelTimer),this.mousewheelTimer=this._delay(function(){this.spinning&&this._stop(t)},100),t.preventDefault()}},"mousedown .ui-spinner-button":function(e){function i(){var e=this.element[0]===t.ui.safeActiveElement(this.document[0]);e||(this.element.trigger("focus"),this.previous=s,this._delay(function(){this.previous=s}))}var s;s=this.element[0]===t.ui.safeActiveElement(this.document[0])?this.previous:this.element.val(),e.preventDefault(),i.call(this),this.cancelBlur=!0,this._delay(function(){delete this.cancelBlur,i.call(this)}),this._start(e)!==!1&&this._repeat(null,t(e.currentTarget).hasClass("ui-spinner-up")?1:-1,e)},"mouseup .ui-spinner-button":"_stop","mouseenter .ui-spinner-button":function(e){return t(e.currentTarget).hasClass("ui-state-active")?this._start(e)===!1?!1:(this._repeat(null,t(e.currentTarget).hasClass("ui-spinner-up")?1:-1,e),void 0):void 0},"mouseleave .ui-spinner-button":"_stop"},_enhance:function(){this.uiSpinner=this.element.attr("autocomplete","off").wrap("<span>").parent().append("<a></a><a></a>")},_draw:function(){this._enhance(),this._addClass(this.uiSpinner,"ui-spinner","ui-widget ui-widget-content"),this._addClass("ui-spinner-input"),this.element.attr("role","spinbutton"),this.buttons=this.uiSpinner.children("a").attr("tabIndex",-1).attr("aria-hidden",!0).button({classes:{"ui-button":""}}),this._removeClass(this.buttons,"ui-corner-all"),this._addClass(this.buttons.first(),"ui-spinner-button ui-spinner-up"),this._addClass(this.buttons.last(),"ui-spinner-button ui-spinner-down"),this.buttons.first().button({icon:this.options.icons.up,showLabel:!1}),this.buttons.last().button({icon:this.options.icons.down,showLabel:!1}),this.buttons.height()>Math.ceil(.5*this.uiSpinner.height())&&this.uiSpinner.height()>0&&this.uiSpinner.height(this.uiSpinner.height())},_keydown:function(e){var i=this.options,s=t.ui.keyCode;switch(e.keyCode){case s.UP:return this._repeat(null,1,e),!0;case s.DOWN:return this._repeat(null,-1,e),!0;case s.PAGE_UP:return this._repeat(null,i.page,e),!0;case s.PAGE_DOWN:return this._repeat(null,-i.page,e),!0}return!1},_start:function(t){return this.spinning||this._trigger("start",t)!==!1?(this.counter||(this.counter=1),this.spinning=!0,!0):!1},_repeat:function(t,e,i){t=t||500,clearTimeout(this.timer),this.timer=this._delay(function(){this._repeat(40,e,i)},t),this._spin(e*this.options.step,i)},_spin:function(t,e){var i=this.value()||0;this.counter||(this.counter=1),i=this._adjustValue(i+t*this._increment(this.counter)),this.spinning&&this._trigger("spin",e,{value:i})===!1||(this._value(i),this.counter++)},_increment:function(e){var i=this.options.incremental;return i?t.isFunction(i)?i(e):Math.floor(e*e*e/5e4-e*e/500+17*e/200+1):1},_precision:function(){var t=this._precisionOf(this.options.step);return null!==this.options.min&&(t=Math.max(t,this._precisionOf(this.options.min))),t},_precisionOf:function(t){var e=""+t,i=e.indexOf(".");return-1===i?0:e.length-i-1},_adjustValue:function(t){var e,i,s=this.options;return e=null!==s.min?s.min:0,i=t-e,i=Math.round(i/s.step)*s.step,t=e+i,t=parseFloat(t.toFixed(this._precision())),null!==s.max&&t>s.max?s.max:null!==s.min&&s.min>t?s.min:t},_stop:function(t){this.spinning&&(clearTimeout(this.timer),clearTimeout(this.mousewheelTimer),this.counter=0,this.spinning=!1,this._trigger("stop",t))},_setOption:function(t,e){var i,s,n;return"culture"===t||"numberFormat"===t?(i=this._parse(this.element.val()),this.options[t]=e,this.element.val(this._format(i)),void 0):(("max"===t||"min"===t||"step"===t)&&"string"==typeof e&&(e=this._parse(e)),"icons"===t&&(s=this.buttons.first().find(".ui-icon"),this._removeClass(s,null,this.options.icons.up),this._addClass(s,null,e.up),n=this.buttons.last().find(".ui-icon"),this._removeClass(n,null,this.options.icons.down),this._addClass(n,null,e.down)),this._super(t,e),void 0)},_setOptionDisabled:function(t){this._super(t),this._toggleClass(this.uiSpinner,null,"ui-state-disabled",!!t),this.element.prop("disabled",!!t),this.buttons.button(t?"disable":"enable")},_setOptions:r(function(t){this._super(t)}),_parse:function(t){return"string"==typeof t&&""!==t&&(t=window.Globalize&&this.options.numberFormat?Globalize.parseFloat(t,10,this.options.culture):+t),""===t||isNaN(t)?null:t},_format:function(t){return""===t?"":window.Globalize&&this.options.numberFormat?Globalize.format(t,this.options.numberFormat,this.options.culture):t},_refresh:function(){this.element.attr({"aria-valuemin":this.options.min,"aria-valuemax":this.options.max,"aria-valuenow":this._parse(this.element.val())})},isValid:function(){var t=this.value();return null===t?!1:t===this._adjustValue(t)},_value:function(t,e){var i;""!==t&&(i=this._parse(t),null!==i&&(e||(i=this._adjustValue(i)),t=this._format(i))),this.element.val(t),this._refresh()},_destroy:function(){this.element.prop("disabled",!1).removeAttr("autocomplete role aria-valuemin aria-valuemax aria-valuenow"),this.uiSpinner.replaceWith(this.element)},stepUp:r(function(t){this._stepUp(t)}),_stepUp:function(t){this._start()&&(this._spin((t||1)*this.options.step),this._stop())},stepDown:r(function(t){this._stepDown(t)}),_stepDown:function(t){this._start()&&(this._spin((t||1)*-this.options.step),this._stop())},pageUp:r(function(t){this._stepUp((t||1)*this.options.page)}),pageDown:r(function(t){this._stepDown((t||1)*this.options.page)}),value:function(t){return arguments.length?(r(this._value).call(this,t),void 0):this._parse(this.element.val())},widget:function(){return 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s=this;this._super(e,i),"content"===e&&t.each(this.tooltips,function(t,e){s._updateContent(e.element)})},_setOptionDisabled:function(t){this[t?"_disable":"_enable"]()},_disable:function(){var e=this;t.each(this.tooltips,function(i,s){var n=t.Event("blur");n.target=n.currentTarget=s.element[0],e.close(n,!0)}),this.disabledTitles=this.disabledTitles.add(this.element.find(this.options.items).addBack().filter(function(){var e=t(this);return e.is("[title]")?e.data("ui-tooltip-title",e.attr("title")).removeAttr("title"):void 0}))},_enable:function(){this.disabledTitles.each(function(){var e=t(this);e.data("ui-tooltip-title")&&e.attr("title",e.data("ui-tooltip-title"))}),this.disabledTitles=t([])},open:function(e){var i=this,s=t(e?e.target:this.element).closest(this.options.items);s.length&&!s.data("ui-tooltip-id")&&(s.attr("title")&&s.data("ui-tooltip-title",s.attr("title")),s.data("ui-tooltip-open",!0),e&&"mouseover"===e.type&&s.parents().each(function(){var 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s=t.Event(e);s.currentTarget=i[0],this.close(s,!0)}}};i[0]!==this.element[0]&&(s.remove=function(){this._removeTooltip(this._find(i).tooltip)}),e&&"mouseover"!==e.type||(s.mouseleave="close"),e&&"focusin"!==e.type||(s.focusout="close"),this._on(!0,i,s)},close:function(e){var i,s=this,n=t(e?e.currentTarget:this.element),o=this._find(n);return o?(i=o.tooltip,o.closing||(clearInterval(this.delayedShow),n.data("ui-tooltip-title")&&!n.attr("title")&&n.attr("title",n.data("ui-tooltip-title")),this._removeDescribedBy(n),o.hiding=!0,i.stop(!0),this._hide(i,this.options.hide,function(){s._removeTooltip(t(this))}),n.removeData("ui-tooltip-open"),this._off(n,"mouseleave focusout keyup"),n[0]!==this.element[0]&&this._off(n,"remove"),this._off(this.document,"mousemove"),e&&"mouseleave"===e.type&&t.each(this.parents,function(e,i){t(i.element).attr("title",i.title),delete s.parents[e]}),o.closing=!0,this._trigger("close",e,{tooltip:i}),o.hiding||(o.closing=!1)),void 0):(n.removeData("ui-tooltip-open"),void 0)},_tooltip:function(e){var i=t("<div>").attr("role","tooltip"),s=t("<div>").appendTo(i),n=i.uniqueId().attr("id");return this._addClass(s,"ui-tooltip-content"),this._addClass(i,"ui-tooltip","ui-widget ui-widget-content"),i.appendTo(this._appendTo(e)),this.tooltips[n]={element:e,tooltip:i}},_find:function(t){var e=t.data("ui-tooltip-id");return e?this.tooltips[e]:null},_removeTooltip:function(t){t.remove(),delete this.tooltips[t.attr("id")]},_appendTo:function(t){var e=t.closest(".ui-front, dialog");return e.length||(e=this.document[0].body),e},_destroy:function(){var e=this;t.each(this.tooltips,function(i,s){var n=t.Event("blur"),o=s.element;n.target=n.currentTarget=o[0],e.close(n,!0),t("#"+i).remove(),o.data("ui-tooltip-title")&&(o.attr("title")||o.attr("title",o.data("ui-tooltip-title")),o.removeData("ui-tooltip-title"))}),this.liveRegion.remove()}}),t.uiBackCompat!==!1&&t.widget("ui.tooltip",t.ui.tooltip,{options:{tooltipClass:null},_tooltip:function(){var t=this._superApply(arguments);return this.options.tooltipClass&&t.tooltip.addClass(this.options.tooltipClass),t}}),t.ui.tooltip}); | ||
+ | </script> | ||
Line 14: | Line 41: | ||
main { | main { | ||
width:86vw; | width:86vw; | ||
− | } | + | } |
+ | |||
+ | .ui-accordion .ui-accordion-content { | ||
+ | max-height:calc(100vh - 10rem); | ||
+ | } | ||
.ui-accordion-content .p, .ui-accordion .ui-tabs { | .ui-accordion-content .p, .ui-accordion .ui-tabs { | ||
Line 23: | Line 54: | ||
display:flex; | display:flex; | ||
justify-content: space-around; | justify-content: space-around; | ||
+ | align-content:space-around; | ||
flex-wrap: wrap; | flex-wrap: wrap; | ||
} | } | ||
− | . | + | .flex-container table, .flex-container figure { |
− | + | margin:auto; | |
} | } | ||
− | + | table { | |
− | + | padding:0; | |
} | } | ||
− | + | table caption { | |
− | border-top: 0. | + | border-top: 0.1rem solid; |
− | border-bottom: 0. | + | border-bottom: 0.1rem solid; |
margin: auto; | margin: auto; | ||
padding: 0.2rem 0; | padding: 0.2rem 0; | ||
Line 42: | Line 74: | ||
} | } | ||
− | + | table tr { | |
margin: 0; | margin: 0; | ||
padding: 0; | padding: 0; | ||
} | } | ||
− | + | table td { | |
border:none; | border:none; | ||
margin: 0; | margin: 0; | ||
Line 53: | Line 85: | ||
} | } | ||
− | + | #accordion table tr:last-child td { | |
− | border-bottom: 0. | + | border-bottom: 0.1rem solid; |
} | } | ||
− | + | figure { | |
text-align: center; | text-align: center; | ||
+ | padding:0; | ||
} | } | ||
− | + | figure figcaption { | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
font-size: 0.8rem; | font-size: 0.8rem; | ||
margin: 0; | margin: 0; | ||
Line 75: | Line 103: | ||
/*PCR system*/ | /*PCR system*/ | ||
.table-theme-1 { | .table-theme-1 { | ||
− | color: rgb(49,132,155); | + | color: rgb(49,132,155); |
− | + | ||
} | } | ||
Line 605: | Line 632: | ||
Cultured the last generation at medium with 5-fluorouracil to select double-crossover clones. Regretfully, we didn't get the double-crossover clones. | Cultured the last generation at medium with 5-fluorouracil to select double-crossover clones. Regretfully, we didn't get the double-crossover clones. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table2-2-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table2-2-1"> | |
− | + | <caption>20μL PCR system</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-up-F</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-dn-R</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table2-2-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>56℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>2 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/3/3d/T--NKU_China--notebook-1-5-3.png" width="341" height="292"> | |
− | + | <figcaption> | |
− | + | Selecting double-crossover clones using PCR<br> | |
− | + | (No.1-5 are experimental groups, No.6 is wild GR286.<br> | |
− | + | The result showed that we failed to get the double-crossover clones.) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 692: | Line 721: | ||
We cultured transformants at 42℃ with chloramphenicol again and selected the single-crossover clones successfully. | We cultured transformants at 42℃ with chloramphenicol again and selected the single-crossover clones successfully. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table3-1-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table3-1-1"> | |
− | + | <caption>20μL PCR system</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-up-F</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-dn-R</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table3-1-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>56℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>2 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/9/93/T--NKU_China--notebook-1-5-4.png" width="350" height="292"> | |
− | + | <figcaption> | |
− | + | Selecting single-crossover clones using PCR<br> | |
− | + | (No.1&2&4 are single-crossover strains,No.5 is positive control. ) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment3-2"> | <div id="fragment3-2"> | ||
Line 769: | Line 800: | ||
Cultured the last generation at medium with 5-fluorouracil to select double-crossover clones. We finally got our aimed strain—GR286Δ<i>luxS</i>. | Cultured the last generation at medium with 5-fluorouracil to select double-crossover clones. We finally got our aimed strain—GR286Δ<i>luxS</i>. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table3-3-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table3-3-1"> | |
− | + | <caption>20μL PCR system</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-up-F</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-dn-R</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table3-3-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>56℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>2 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--NKU_China--notebook-1-5-5.png" width="254" height="209"> | |
− | + | <figcaption> | |
− | + | Selecting the strain lacking of <i>luxS</i> gene using PCR<br> | |
− | + | (The No.4 is the aimed strain<U+00A1><U+00AA>GR286Δ<i>luxS</i>) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 863: | Line 896: | ||
Cloned the <i>lsrACDB</i> gene from <i>Bacillus thuringiensis</i> and ligated it to T-vector. | Cloned the <i>lsrACDB</i> gene from <i>Bacillus thuringiensis</i> and ligated it to T-vector. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table4-2-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table4-2-1"> | |
− | + | <caption>50μL PCR system ×2</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>lsrACDB</i>-F</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>lsrACDB</i>-R</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>19μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>50μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table4-2-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>57℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>4 min 30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-3" id="table4-2-3"> | |
− | + | <caption>20μL ligation system</caption> | |
− | + | <tr> | |
− | + | <td>10× DNA Ligase Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T4 DNA Ligase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pMD19 T-Simple Vector</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>lsrACDB</i></td> | |
− | + | <td>3μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>13μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 16℃ overnight</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment4-3"> | <div id="fragment4-3"> | ||
Line 958: | Line 993: | ||
Transformed the T-<i>lsrACDB</i> into DH5α and coated plate, and then selected positive clones by colony PCR. | Transformed the T-<i>lsrACDB</i> into DH5α and coated plate, and then selected positive clones by colony PCR. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table4-3-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table4-3-1"> | |
− | + | <caption>20μL PCR system</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>M13F</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>M13R</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table4-3-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>59℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>4 min 30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/7/72/T--NKU_China--notebook-1-5-6.png" width="215" height="180"> | |
− | + | <figcaption> | |
− | + | Selecting positive clones by PCR<br> | |
− | + | (No. 3&4 are positive results) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment4-4"> | <div id="fragment4-4"> | ||
Line 1,030: | Line 1,067: | ||
After restriction enzyme digestion verification, we sent them to sequencing. Unfortunately, the sequencing result showed some mutations in cloning gene. | After restriction enzyme digestion verification, we sent them to sequencing. Unfortunately, the sequencing result showed some mutations in cloning gene. | ||
</div> | </div> | ||
− | <table class="table-theme-4" id="table4-4-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-4" id="table4-4-1"> | |
− | + | <caption>20μL digestion system</caption> | |
− | + | <tr> | |
− | + | <td>10× FastDigest Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T-<i>lsrACDB</i></td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 40 min</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/9/91/T--NKU_China--notebook-1-5-7.png" width="201" height="322"> | |
− | + | <figcaption> | |
− | + | Restriction enzyme digestion verification<br> | |
− | + | (No.1 are <i>lsrACDB</i> fragement, No.2 are linearized T-vector.) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment4-5"> | <div id="fragment4-5"> | ||
Line 1,096: | Line 1,135: | ||
We cloned a strong promoter <i>C2</i> from former kit and cloned <i>luxS</i> gene from GR286. | We cloned a strong promoter <i>C2</i> from former kit and cloned <i>luxS</i> gene from GR286. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table5-1-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table5-1-1"> | |
− | + | <caption>50μL PCR system ×2</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>C2-F</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>C2-R</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>p-C2</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>19μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>50μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table5-1-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>58℃</td> | |
− | + | <td>15 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-1" id="table5-1-3"> | |
− | + | <caption>50μL PCR system ×2</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-F</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-R</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>GR286</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>19μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>50μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table5-1-4"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>59℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/d/dc/T--NKU_China--notebook-1-5-8.png" width="201" height="322"> | |
− | + | <figcaption> | |
− | + | Cloning promoter <i>C2</i> and <i>luxS</i> using PCR<br> | |
− | + | (NO.1&2 are <i>C2</i>, No.3&4 are <i>luxS</i>) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment5-2"> | <div id="fragment5-2"> | ||
Line 1,232: | Line 1,273: | ||
Fuse the two segments together by fusion PCR, and ligated it into T-vector. Then, transformed the vector into DH5α. | Fuse the two segments together by fusion PCR, and ligated it into T-vector. Then, transformed the vector into DH5α. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table5-2-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table5-2-1"> | |
− | + | <caption>50μL PCR system ×2</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>C2-F</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i>-R</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>C2</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i></td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>17μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>50μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table5-2-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>59℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>40 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-3" id="table5-2-3"> | |
− | + | <caption>20μL ligation system</caption> | |
− | + | <tr> | |
− | + | <td>10× DNA Ligase Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T4 DNA Ligase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pMD19 T-Simple Vector</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>C2-luxS</i></td> | |
− | + | <td>4μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>12μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 16℃ overnight</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment5-3"> | <div id="fragment5-3"> | ||
Line 1,331: | Line 1,374: | ||
Selected the positive clones by colony PCR. | Selected the positive clones by colony PCR. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table5-3-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table5-3-1"> | |
− | + | <caption>20μL PCR system</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>M13-F</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>M13-R</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table5-3-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>59℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>40 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/c/c9/T--NKU_China--notebook-1-5-9.png" width="308" height="303"> | |
− | + | <figcaption> | |
− | + | Selecting positive clones by colony PCR | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment5-4"> | <div id="fragment5-4"> | ||
Line 1,402: | Line 1,447: | ||
We chose 4 positive strains to culture overnight and extracted the plasmid. After restriction enzyme digestion verification, we sent them to sequencing. | We chose 4 positive strains to culture overnight and extracted the plasmid. After restriction enzyme digestion verification, we sent them to sequencing. | ||
</div> | </div> | ||
− | <table class="table-theme-4" id="table5-4-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-4" id="table5-4-1"> | |
− | + | <caption>20μL digestion system</caption> | |
− | + | <tr> | |
− | + | <td>10× FastDigest Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T-<i>lsrACDB</i></td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 40 min</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/f/fb/T--NKU_China--notebook-1-5-10.png" width="317" height="202"> | |
− | + | <figcaption> | |
− | + | Restriction enzyme digestion verification<br> | |
− | + | (No.1 are linearized T-vector, No.2 are <i>C2-luxS</i> fragment.) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 1,456: | Line 1,503: | ||
The sequencing result showed there's a correct strain. So we can use the strain for the following experiment. We obtained the correct plasmid T-<i>C2-luxS</i> from DH5α. Then we got the fragment <i>C2-luxS</i> by digestion and gel extraction. | The sequencing result showed there's a correct strain. So we can use the strain for the following experiment. We obtained the correct plasmid T-<i>C2-luxS</i> from DH5α. Then we got the fragment <i>C2-luxS</i> by digestion and gel extraction. | ||
</div> | </div> | ||
− | <table class="table-theme-4" id="table6-1-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-4" id="table6-1-1"> | |
− | + | <caption>40μL digestion system</caption> | |
− | + | <tr> | |
− | + | <td>10× FastDigest Buffer</td> | |
− | + | <td>4μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T-<i>C2-luxS</i></td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>9μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 40 min</td> | |
− | + | </tr> | |
− | + | </table> | |
+ | </div> | ||
</div> | </div> | ||
<div id="fragment6-2"> | <div id="fragment6-2"> | ||
Line 1,488: | Line 1,536: | ||
Ligated the <i>C2-luxS</i> to linearized plasmid pWH1520, and transformed it into DH5α. | Ligated the <i>C2-luxS</i> to linearized plasmid pWH1520, and transformed it into DH5α. | ||
</div> | </div> | ||
− | <table class="table-theme-3" id="table6-2-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-3" id="table6-2-1"> | |
− | + | <caption>20μL ligation system</caption> | |
− | + | <tr> | |
− | + | <td>10× DNA Ligase Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T4 DNA Ligase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pMD19 T-Simple Vector</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>C2-luxS</i></td> | |
− | + | <td>5μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>11μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 16℃ overnight</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment6-3"> | <div id="fragment6-3"> | ||
Line 1,528: | Line 1,578: | ||
Extracted the plasmid pWH-<i>C2-luxS</i> from JM110,and dealt with it by BamH Ⅰ methylase. | Extracted the plasmid pWH-<i>C2-luxS</i> from JM110,and dealt with it by BamH Ⅰ methylase. | ||
</div> | </div> | ||
− | <table class="table-theme-5" id="table6-4-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-5" id="table6-4-1"> | |
− | + | <caption>100μL methylation system</caption> | |
− | + | <tr> | |
− | + | <td>10× BamH Ⅰ methyltransferase Buffer</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ methyltransferase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>S-adenosylmethionine</td> | |
− | + | <td>0.5μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-<i>C2-luxS</i></td> | |
− | + | <td>80μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>8.5μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>100μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 1 hour</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment6-5"> | <div id="fragment6-5"> | ||
Line 1,563: | Line 1,615: | ||
Transformed the plasmid into GR286 by electroporation.[Failed] | Transformed the plasmid into GR286 by electroporation.[Failed] | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/e/e0/T--NKU_China--notebook-6-13-1.png" width="347" height="284"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/e/e0/T--NKU_China--notebook-6-13-1.png" width="347" height="284"> | |
− | + | <figcaption> | |
− | + | Selecting positive clones by colony PCR<br> | |
− | + | (No.1 is positive control, No.2-6 are experimental groups.<br> | |
− | + | The result showed that we failed to transformed the plasmid pWH-<i>C2-luxS</i> into GR286) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 1,590: | Line 1,644: | ||
This week, we tried to use different voltages to transform the plasmid. Sadly, all of these tries got bad results. | This week, we tried to use different voltages to transform the plasmid. Sadly, all of these tries got bad results. | ||
</div> | </div> | ||
− | |||
</div> | </div> | ||
<div id="fragment7-2"> | <div id="fragment7-2"> | ||
Line 1,596: | Line 1,649: | ||
We considered whether the <i>luxS</i> gene is a little toxic for GR286, and the bacteria tends to refuse the gene when we added a strong promoter in front of it. So, we planned to use induced expression to reconstruction our expression vector. | We considered whether the <i>luxS</i> gene is a little toxic for GR286, and the bacteria tends to refuse the gene when we added a strong promoter in front of it. So, we planned to use induced expression to reconstruction our expression vector. | ||
</div> | </div> | ||
− | |||
</div> | </div> | ||
<div id="fragment7-3"> | <div id="fragment7-3"> | ||
Line 1,602: | Line 1,654: | ||
The plasmid pWH1520 contains the strong <i>xylA</i> promoter originating, and transcription from this promoter is xylose inducible. So, the gene of interest carries its own ribosome binding sequence (RBS) and translation initiation codon. Based on these points, we redesigned primers. | The plasmid pWH1520 contains the strong <i>xylA</i> promoter originating, and transcription from this promoter is xylose inducible. So, the gene of interest carries its own ribosome binding sequence (RBS) and translation initiation codon. Based on these points, we redesigned primers. | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/e/eb/T--NKU_China--notebook-6-13-2.png" width="654" height="652"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/e/eb/T--NKU_China--notebook-6-13-2.png" width="654" height="652"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 1,627: | Line 1,681: | ||
We cloned <i>luxS</i> gene from GR286 using our new primers. | We cloned <i>luxS</i> gene from GR286 using our new primers. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table8-1-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table8-1-1"> | |
− | + | <caption>50μL PCR system ×2</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>YD-luxS-F</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>YD-luxS-R</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>19μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>50μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table8-1-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>58℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>40 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/3/3a/T--NKU_China--notebook-6-13-3.png" width="200" height="348"> | |
− | + | <figcaption> | |
− | + | Cloning <i>luxS</i> gene by PCR | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment8-2"> | <div id="fragment8-2"> | ||
Line 1,698: | Line 1,754: | ||
Purified the <i>luxS</i> fragment by gel extraction, and ligated it into linearized pWH1520. Then, transformed the vector into DH5α. | Purified the <i>luxS</i> fragment by gel extraction, and ligated it into linearized pWH1520. Then, transformed the vector into DH5α. | ||
</div> | </div> | ||
− | <table class="table-theme-4" id="table8-2-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-4" id="table8-2-1"> | |
− | + | <caption>40μL digestion system ×2</caption> | |
− | + | <tr> | |
− | + | <td>10× FastDigest Buffer</td> | |
− | + | <td>4μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH1520</td> | |
− | + | <td>25μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>9μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>40μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 40 min</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-3" id="table8-2-2"> | |
− | + | <caption>20μL ligation system</caption> | |
− | + | <tr> | |
− | + | <td>10× DNA Ligase Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T4 DNA Ligase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>linearized pWH1520</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>luxS</i></td> | |
− | + | <td>3μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>13μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 16℃ overnight</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment8-3"> | <div id="fragment8-3"> | ||
Line 1,759: | Line 1,817: | ||
Selected the positive clones by colony PCR. | Selected the positive clones by colony PCR. | ||
</div> | </div> | ||
− | <table class="table-theme-1" id="table8-3-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-1" id="table8-3-1"> | |
− | + | <caption>20μL PCR system</caption> | |
− | + | <tr> | |
− | + | <td>2× Taq Master Mix</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-F</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-R</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Bacterium solution</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <table class="table-theme-2" id="table8-3-2"> | |
− | + | <caption>PCR reaction condition</caption> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>94℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>58℃</td> | |
− | + | <td>30 sec</td> | |
− | + | <td>30 cycles</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>40 sec</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>72℃</td> | |
− | + | <td>10 min</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>16℃</td> | |
− | + | <td>∞</td> | |
− | + | <td></td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/4/49/T--NKU_China--notebook-6-13-4.png" width="347" height="284"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment8-4"> | <div id="fragment8-4"> | ||
Line 1,829: | Line 1,889: | ||
We chose 4 positive strains to culture overnight and extracted the plasmid. After restriction enzyme digestion verification, we sent them to sequencing. | We chose 4 positive strains to culture overnight and extracted the plasmid. After restriction enzyme digestion verification, we sent them to sequencing. | ||
</div> | </div> | ||
− | <table class="table-theme-4" id="table8-4-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-4" id="table8-4-1"> | |
− | + | <caption>20μL digestion system</caption> | |
− | + | <tr> | |
− | + | <td>10× FastDigest Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-<i>luxs</i></td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>7μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 40 min</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/3/35/T--NKU_China--notebook-6-13-5.png" width="347" height="284"> | |
− | + | <figcaption> | |
− | + | Restriction enzyme digestion verification<br> | |
− | + | (No.1 are linearized pWH1520, No.2 are <i>luxS</i> fragments.) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
Line 1,888: | Line 1,950: | ||
Extracted the plasmid pWH -<i>luxS</i> from JM110,and dealt with it by BamH Ⅰ methylase. | Extracted the plasmid pWH -<i>luxS</i> from JM110,and dealt with it by BamH Ⅰ methylase. | ||
</div> | </div> | ||
− | <table class="table-theme-5" id="table6-4-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-5" id="table6-4-1"> | |
− | + | <caption>100μL methylation system</caption> | |
− | + | <tr> | |
− | + | <td>10× BamH Ⅰ methyltransferase Buffer</td> | |
− | + | <td>10μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>BamH Ⅰ methyltransferase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>S-adenosylmethionine</td> | |
− | + | <td>0.5μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-<i>C2-luxS</i></td> | |
− | + | <td>80μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>8.5μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>100μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 37℃ for 1 hour</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment9-3"> | <div id="fragment9-3"> | ||
Line 1,923: | Line 1,987: | ||
Transformed the plasmid into GR286 by electroporation, and selected positive clones. | Transformed the plasmid into GR286 by electroporation, and selected positive clones. | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--NKU_China--notebook-6-13-6.png" width="347" height="284"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/a/aa/T--NKU_China--notebook-6-13-6.png" width="347" height="284"> | |
− | + | <figcaption> | |
− | + | Selecting positive clones by colony PCR | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 1,950: | Line 2,016: | ||
Our synthetic <i>lsrACDB</i> gene came back. We first used restriction-ligation method to ligate <i>lsrACDB</i> to plasmid pWH1520, but we failed to select positive after several tries. | Our synthetic <i>lsrACDB</i> gene came back. We first used restriction-ligation method to ligate <i>lsrACDB</i> to plasmid pWH1520, but we failed to select positive after several tries. | ||
</div> | </div> | ||
− | <table class="table-theme-3" id="table10-1-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-3" id="table10-1-1"> | |
− | + | <caption>20μL ligation system</caption> | |
− | + | <tr> | |
− | + | <td>10× DNA Ligase Buffer</td> | |
− | + | <td>2μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>T4 DNA Ligase</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>linearized pWH1520</td> | |
− | + | <td>1μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td><i>lsrACDB</i></td> | |
− | + | <td>3μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>ddH2O</td> | |
− | + | <td>13μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>Total</td> | |
− | + | <td>20μL</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td colspan="2">Reaction condition: 16℃ overnight</td> | |
− | + | </tr> | |
+ | </table> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment10-2"> | <div id="fragment10-2"> | ||
Line 1,985: | Line 2,053: | ||
Considering the <i>lsrACDB</i> gene is a large fragment (4500bp), we used ClonExpress technique cloning the gene again to improve the efficiency of ligation. We divided the <i>lsrACDB</i> sequence into two parts and cloned them separately. Then we ligated the two segments to the plasmid pWH1520 and transformed it into DH5α. After that, we used PCR to select the positive clones. However, we didn't get a good result. | Considering the <i>lsrACDB</i> gene is a large fragment (4500bp), we used ClonExpress technique cloning the gene again to improve the efficiency of ligation. We divided the <i>lsrACDB</i> sequence into two parts and cloned them separately. Then we ligated the two segments to the plasmid pWH1520 and transformed it into DH5α. After that, we used PCR to select the positive clones. However, we didn't get a good result. | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/1/17/T--NKU_China--notebook-6-13-7.png" width="285" height="207"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/1/17/T--NKU_China--notebook-6-13-7.png" width="285" height="207"> | |
− | + | <figcaption> | |
− | + | Selecting positive clones by colony PCR<br> | |
− | + | (No.6 is positive control, No.1-5 are experimental groups) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 2,004: | Line 2,074: | ||
We learnt a new method called circular polymerase extension cloning (CPEC) for high-throughput cloning of complex and combinatorial DNA libraries, and we decided to use this method to try ligating our <i>lsrACDB</i> gene. It's encouraging that we succeeded to ligate the <i>lsrACDB</i> gene to the plasmid pHT-01. | We learnt a new method called circular polymerase extension cloning (CPEC) for high-throughput cloning of complex and combinatorial DNA libraries, and we decided to use this method to try ligating our <i>lsrACDB</i> gene. It's encouraging that we succeeded to ligate the <i>lsrACDB</i> gene to the plasmid pHT-01. | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/c/c2/T--NKU_China--notebook-6-13-8.png" width="396" height="322"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/c/c2/T--NKU_China--notebook-6-13-8.png" width="396" height="322"> | |
− | + | <figcaption> | |
− | + | Selecting positive clones by colony PCR<br> | |
− | + | (No.3-6 are positive clones) | |
− | + | </figcaption> | |
− | + | </figure> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/7/7a/T--NKU_China--notebook-6-13-9.png" width="319" height="409"> | |
− | + | <figcaption> | |
− | + | Restriction enzyme digestion verification<br> | |
− | + | (No.2&3 are positive results.) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 2,034: | Line 2,106: | ||
Since we have constructed "supplier" and part of "consumer", we decided to measure the growth curve to explore the function of our "controller". | Since we have constructed "supplier" and part of "consumer", we decided to measure the growth curve to explore the function of our "controller". | ||
</div> | </div> | ||
− | <table class="table-theme-6" id="table12-1-1"> | + | <div class="flex-container"> |
− | + | <table class="table-theme-6" id="table12-1-1"> | |
− | + | <caption>Groups divided in this experiment</caption> | |
− | + | <tr> | |
− | + | <td>GR286</td> | |
− | + | <td>wild strain as control group</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>GR286Δ<i>luxS</i></td> | |
− | + | <td>GR286 without <i>luxS</i> gene</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-<i>luxS</i></td> | |
− | + | <td><i>luxS</i> overexpression plasmid in GR286; without induced by xylose</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH-<i>luxS</i> + xyl</td> | |
− | + | <td><i>luxS</i> overexpression plasmid in GR286; induced by xylose</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pWH1520</td> | |
− | + | <td>empty plasmid in GR286 as control group</td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pHT-<i>lsrACDB</i></td> | |
− | + | <td><i>lsrACDB</i> overexpression plasmid in GR286Δ<i>luxS</i></td> | |
− | + | </tr> | |
− | + | <tr> | |
− | + | <td>pHT-01</td> | |
− | + | <td>empty plasmid in GR286Δ<i>luxS</i> as control group</td> | |
− | + | </tr> | |
− | + | </table> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/a/ad/T--NKU_China--notebook-6-13-10.png" width="779" height="298"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
− | + | </figure> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/0/00/T--NKU_China--notebook-6-13-11.png" width="596" height="359"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
− | + | </figure> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/a/a9/T--NKU_China--notebook-6-13-12.png" width="584" height="349"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
− | + | </figure> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/6/6b/T--NKU_China--notebook-6-13-13.png" width="579" height="354"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment12-2"> | <div id="fragment12-2"> | ||
Line 2,090: | Line 2,164: | ||
Cultured media of our supplier was tested for the presence of AI-2 by inducing luminescence in <i>Vibrio harveyi</i> reporter strain BB170. | Cultured media of our supplier was tested for the presence of AI-2 by inducing luminescence in <i>Vibrio harveyi</i> reporter strain BB170. | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/a/a4/T--NKU_China--notebook-6-13-14.png" width="485" height="341"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/a/a4/T--NKU_China--notebook-6-13-14.png" width="485" height="341"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
− | + | </figure> | |
− | + | <figure> | |
− | + | <img src="https://static.igem.org/mediawiki/2016/c/c5/T--NKU_China--notebook-6-13-15.png" width="608" height="72"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
Line 2,118: | Line 2,194: | ||
For our consumer, we should also clone the <i>lsrK</i> and <i>lsrFG</i> gene for phosphorylating and degrading AI-2. We used ClonExpress technique cloning the two genes and ligated them to plasmid pHT-01 successfully. | For our consumer, we should also clone the <i>lsrK</i> and <i>lsrFG</i> gene for phosphorylating and degrading AI-2. We used ClonExpress technique cloning the two genes and ligated them to plasmid pHT-01 successfully. | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/c/c5/T--NKU_China--notebook-6-13-16.png" width="429" height="320"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/c/c5/T--NKU_China--notebook-6-13-16.png" width="429" height="320"> | |
− | + | <figcaption> | |
− | + | Restriction enzyme digestion verification<br> | |
− | + | (No.1&3&4 are positive results. ) | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
<div id="fragment12-2"> | <div id="fragment12-2"> | ||
Line 2,130: | Line 2,208: | ||
We cocultured the supplier with BB170 and tested the fluoresent intensity to explore the function of supplier. (negative result) | We cocultured the supplier with BB170 and tested the fluoresent intensity to explore the function of supplier. (negative result) | ||
</div> | </div> | ||
− | < | + | <div class="flex-container"> |
− | <img src="https://static.igem.org/mediawiki/2016/f/f1/T--NKU_China--notebook-6-13-17.png" width="424" height="272"> | + | <figure> |
− | + | <img src="https://static.igem.org/mediawiki/2016/f/f1/T--NKU_China--notebook-6-13-17.png" width="424" height="272"> | |
− | + | <figcaption> | |
− | + | </figcaption> | |
+ | </figure> | ||
+ | </div> | ||
</div> | </div> | ||
</div> | </div> |
Revision as of 01:33, 25 August 2016
Laboratory Notes
☞☟ Week1 (May 16–May 22)
In order to make sure our "consumer" efficient, we should first knock out the luxS gene in our engineering bacteria GR286(a simplified strain of Bacillus amyloliquefaciens LL3). We used a markerless gene replacement method to knock out the luxS gene.
Construction of targeting vector : the upstream and downstream of luxS gene were combined by over-lapping PCR and ligated into plasmid pKSU.
Transformed pKSU-ΔluxS into GR286, and selected out positive clones.
2× Taq Master Mix | 10μL |
pKSU-F | 1μL |
pKSU-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
58℃ | 30 sec | 30 cycles |
72℃ | 1 min 30 sec | |
72℃ | 10 min | |
16℃ | ∞ |
The transformants were cultured at 42℃ with chloramphenicol to select single-crossover clones.
2× Taq Master Mix | 10μL |
luxS-up-F | 1μL |
luxS-dn-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
56℃ | 30 sec | 30 cycles |
72℃ | 2 min | |
72℃ | 10 min | |
16℃ | ∞ |
☞☟ Week2 (May 23–May 29)
The single-crossover strains were then cultured at LB medium and passaged every 12 hours for 4 generation.
Cultured the last generation at medium with 5-fluorouracil to select double-crossover clones. Regretfully, we didn't get the double-crossover clones.
2× Taq Master Mix | 10μL |
luxS-up-F | 1μL |
luxS-dn-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
56℃ | 30 sec | 30 cycles |
72℃ | 2 min | |
72℃ | 10 min | |
16℃ | ∞ |
☞☟ Week3 (May 30–Jun 05)
We cultured transformants at 42℃ with chloramphenicol again and selected the single-crossover clones successfully.
2× Taq Master Mix | 10μL |
luxS-up-F | 1μL |
luxS-dn-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
56℃ | 30 sec | 30 cycles |
72℃ | 2 min | |
72℃ | 10 min | |
16℃ | ∞ |
The single-crossover strains were then cultured at LB medium and passaged every 12 hours for 4 generations.
Cultured the last generation at medium with 5-fluorouracil to select double-crossover clones. We finally got our aimed strain—GR286ΔluxS.
2× Taq Master Mix | 10μL |
luxS-up-F | 1μL |
luxS-dn-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
56℃ | 30 sec | 30 cycles |
72℃ | 2 min | |
72℃ | 10 min | |
16℃ | ∞ |
☞☟ Week4 (Jun 06–Jun 12)
Cultured the GR286ΔluxS strain and made it competence for future use.
Cloned the lsrACDB gene from Bacillus thuringiensis and ligated it to T-vector.
2× Taq Master Mix | 25μL |
lsrACDB-F | 2μL |
lsrACDB-R | 2μL |
Bacterium solution | 2μL |
ddH2O | 19μL |
Total | 50μL |
94℃ | 10 min | |
94℃ | 30 sec | |
57℃ | 30 sec | 30 cycles |
72℃ | 4 min 30 sec | |
72℃ | 10 min | |
16℃ | ∞ |
10× DNA Ligase Buffer | 2μL |
T4 DNA Ligase | 1μL |
pMD19 T-Simple Vector | 1μL |
lsrACDB | 3μL |
ddH2O | 13μL |
Total | 20μL |
Reaction condition: 16℃ overnight |
Transformed the T-lsrACDB into DH5α and coated plate, and then selected positive clones by colony PCR.
2× Taq Master Mix | 10μL |
M13F | 1μL |
M13R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
59℃ | 30 sec | 30 cycles |
72℃ | 4 min 30 sec | |
72℃ | 10 min | |
16℃ | ∞ |
After restriction enzyme digestion verification, we sent them to sequencing. Unfortunately, the sequencing result showed some mutations in cloning gene.
10× FastDigest Buffer | 2μL |
BamH Ⅰ | 1μL |
T-lsrACDB | 1μL |
ddH2O | 7μL |
Total | 20μL |
Reaction condition: 37℃ for 40 min |
We repeated the process of gene cloning but there were still some mutations.
We finally decided to request the gene company to synthesize the lsrACDB gene.
☞☟ Week5 (Jun 13–Jun 19)
This week, we started to construct another controller―supplier.
We cloned a strong promoter C2 from former kit and cloned luxS gene from GR286.
2× Taq Master Mix | 25μL |
C2-F | 2μL |
C2-R | 2μL |
p-C2 | 2μL |
ddH2O | 19μL |
Total | 50μL |
94℃ | 10 min | |
94℃ | 30 sec | |
58℃ | 15 sec | 30 cycles |
72℃ | 30 sec | |
72℃ | 10 min | |
16℃ | ∞ |
2× Taq Master Mix | 25μL |
luxS-F | 2μL |
luxS-R | 2μL |
Bacterium solution | 1μL |
GR286 | 2μL |
ddH2O | 19μL |
Total | 50μL |
94℃ | 10 min | |
94℃ | 30 sec | |
59℃ | 30 sec | 30 cycles |
72℃ | 30 sec | |
72℃ | 10 min | |
16℃ | ∞ |
Fuse the two segments together by fusion PCR, and ligated it into T-vector. Then, transformed the vector into DH5α.
2× Taq Master Mix | 25μL |
C2-F | 2μL |
luxS-R | 2μL |
C2 | 2μL |
luxS | 2μL |
ddH2O | 17μL |
Total | 50μL |
94℃ | 10 min | |
94℃ | 30 sec | |
59℃ | 30 sec | 30 cycles |
72℃ | 40 sec | |
72℃ | 10 min | |
16℃ | ∞ |
10× DNA Ligase Buffer | 2μL |
T4 DNA Ligase | 1μL |
pMD19 T-Simple Vector | 1μL |
C2-luxS | 4μL |
ddH2O | 12μL |
Total | 20μL |
Reaction condition: 16℃ overnight |
Selected the positive clones by colony PCR.
2× Taq Master Mix | 10μL |
M13-F | 1μL |
M13-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
59℃ | 30 sec | 30 cycles |
72℃ | 40 sec | |
72℃ | 10 min | |
16℃ | ∞ |
We chose 4 positive strains to culture overnight and extracted the plasmid. After restriction enzyme digestion verification, we sent them to sequencing.
10× FastDigest Buffer | 2μL |
BamH Ⅰ | 1μL |
T-lsrACDB | 1μL |
ddH2O | 7μL |
Total | 20μL |
Reaction condition: 37℃ for 40 min |
☞☟ Week6 (Jun 20–Jun 26)
The sequencing result showed there's a correct strain. So we can use the strain for the following experiment. We obtained the correct plasmid T-C2-luxS from DH5α. Then we got the fragment C2-luxS by digestion and gel extraction.
10× FastDigest Buffer | 4μL |
BamH Ⅰ | 2μL |
T-C2-luxS | 25μL |
ddH2O | 9μL |
Total | 20μL |
Reaction condition: 37℃ for 40 min |
Ligated the C2-luxS to linearized plasmid pWH1520, and transformed it into DH5α.
10× DNA Ligase Buffer | 2μL |
T4 DNA Ligase | 1μL |
pMD19 T-Simple Vector | 1μL |
C2-luxS | 5μL |
ddH2O | 11μL |
Total | 20μL |
Reaction condition: 16℃ overnight |
Extracted the plasmid pWH-C2-luxS from DH5α. To prevent the plasmid from DAM&DCM methylation, we transformed it into E.coli JM110.
Extracted the plasmid pWH-C2-luxS from JM110,and dealt with it by BamH Ⅰ methylase.
10× BamH Ⅰ methyltransferase Buffer | 10μL |
BamH Ⅰ methyltransferase | 1μL |
S-adenosylmethionine | 0.5μL |
pWH-C2-luxS | 80μL |
ddH2O | 8.5μL |
Total | 100μL |
Reaction condition: 37℃ for 1 hour |
Transformed the plasmid into GR286 by electroporation.[Failed]
☞☟ Week7 (Jun 27–Jul 03)
This week, we tried to use different voltages to transform the plasmid. Sadly, all of these tries got bad results.
We considered whether the luxS gene is a little toxic for GR286, and the bacteria tends to refuse the gene when we added a strong promoter in front of it. So, we planned to use induced expression to reconstruction our expression vector.
The plasmid pWH1520 contains the strong xylA promoter originating, and transcription from this promoter is xylose inducible. So, the gene of interest carries its own ribosome binding sequence (RBS) and translation initiation codon. Based on these points, we redesigned primers.
☞☟ Week8 (Jul 04–Jul 10)
We cloned luxS gene from GR286 using our new primers.
2× Taq Master Mix | 25μL |
YD-luxS-F | 2μL |
YD-luxS-R | 2μL |
Bacterium solution | 2μL |
ddH2O | 19μL |
Total | 50μL |
94℃ | 10 min | |
94℃ | 30 sec | |
58℃ | 30 sec | 30 cycles |
72℃ | 40 sec | |
72℃ | 10 min | |
16℃ | ∞ |
Purified the luxS fragment by gel extraction, and ligated it into linearized pWH1520. Then, transformed the vector into DH5α.
10× FastDigest Buffer | 4μL |
BamH Ⅰ | 2μL |
pWH1520 | 25μL |
ddH2O | 9μL |
Total | 40μL |
Reaction condition: 37℃ for 40 min |
10× DNA Ligase Buffer | 2μL |
T4 DNA Ligase | 1μL |
linearized pWH1520 | 1μL |
luxS | 3μL |
ddH2O | 13μL |
Total | 20μL |
Reaction condition: 16℃ overnight |
Selected the positive clones by colony PCR.
2× Taq Master Mix | 10μL |
pWH-F | 1μL |
pWH-R | 1μL |
Bacterium solution | 1μL |
ddH2O | 7μL |
Total | 20μL |
94℃ | 10 min | |
94℃ | 30 sec | |
58℃ | 30 sec | 30 cycles |
72℃ | 40 sec | |
72℃ | 10 min | |
16℃ | ∞ |
We chose 4 positive strains to culture overnight and extracted the plasmid. After restriction enzyme digestion verification, we sent them to sequencing.
10× FastDigest Buffer | 2μL |
BamH Ⅰ | 1μL |
pWH-luxs | 10μL |
ddH2O | 7μL |
Total | 20μL |
Reaction condition: 37℃ for 40 min |
☞☟ Week9 (Jul 11–Jul 17)
The sequencing result showed there's three correct strains. So we can choose a correct strain for the following experiment. We extracted the correct plasmid pWH-luxS from DH5α. To prevent the plasmid from DAM&DCM methylation, we transformed it into E.coli JM110.
Extracted the plasmid pWH -luxS from JM110,and dealt with it by BamH Ⅰ methylase.
10× BamH Ⅰ methyltransferase Buffer | 10μL |
BamH Ⅰ methyltransferase | 1μL |
S-adenosylmethionine | 0.5μL |
pWH-C2-luxS | 80μL |
ddH2O | 8.5μL |
Total | 100μL |
Reaction condition: 37℃ for 1 hour |
Transformed the plasmid into GR286 by electroporation, and selected positive clones.
The construction of supplier was complished!
☞☟ Week10 (Jul 18–Jul 24)
Our synthetic lsrACDB gene came back. We first used restriction-ligation method to ligate lsrACDB to plasmid pWH1520, but we failed to select positive after several tries.
10× DNA Ligase Buffer | 2μL |
T4 DNA Ligase | 1μL |
linearized pWH1520 | 1μL |
lsrACDB | 3μL |
ddH2O | 13μL |
Total | 20μL |
Reaction condition: 16℃ overnight |
Considering the lsrACDB gene is a large fragment (4500bp), we used ClonExpress technique cloning the gene again to improve the efficiency of ligation. We divided the lsrACDB sequence into two parts and cloned them separately. Then we ligated the two segments to the plasmid pWH1520 and transformed it into DH5α. After that, we used PCR to select the positive clones. However, we didn't get a good result.
☞☟ Week11 (Jul 25–Jul 31)
We learnt a new method called circular polymerase extension cloning (CPEC) for high-throughput cloning of complex and combinatorial DNA libraries, and we decided to use this method to try ligating our lsrACDB gene. It's encouraging that we succeeded to ligate the lsrACDB gene to the plasmid pHT-01.
☞☟ Week12 (Aug 1–Aug 7)
Since we have constructed "supplier" and part of "consumer", we decided to measure the growth curve to explore the function of our "controller".
GR286 | wild strain as control group |
GR286ΔluxS | GR286 without luxS gene |
pWH-luxS | luxS overexpression plasmid in GR286; without induced by xylose |
pWH-luxS + xyl | luxS overexpression plasmid in GR286; induced by xylose |
pWH1520 | empty plasmid in GR286 as control group |
pHT-lsrACDB | lsrACDB overexpression plasmid in GR286ΔluxS |
pHT-01 | empty plasmid in GR286ΔluxS as control group |
Cultured media of our supplier was tested for the presence of AI-2 by inducing luminescence in Vibrio harveyi reporter strain BB170.
☞☟ Week13 (Aug 8–Aug 14)
For our consumer, we should also clone the lsrK and lsrFG gene for phosphorylating and degrading AI-2. We used ClonExpress technique cloning the two genes and ligated them to plasmid pHT-01 successfully.
We cocultured the supplier with BB170 and tested the fluoresent intensity to explore the function of supplier. (negative result)
☞☟ Week14 (Aug 15–Aug 21)