Difference between revisions of "Team:HokkaidoU Japan/Notebook/Protocol"

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{{HokkaidoU_Japan}}
 
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<html>
 
<html>
  
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<style>
  
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<br>
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/********************************* DEFAULT WIKI SETTINGS  ********************************/
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<h1>Protocols</h1>
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<a href=https://2016.igem.org/Team:HokkaidoU_Japan/Notebook/notebook>notebook</a>
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#sideMenu, #top_title {display:none;}
 +
#content { padding:0px; width:1000px; margin-top:-7px; margin-left:0px;}
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body {background-color:white; }
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#bodyContent h1, #bodyContent h2, #bodyContent h3, #bodyContent h4, #bodyContent h5 { margin-bottom: 0px; }
  
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<br>
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/********************************* MENU ********************************/
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<h2 onClick="hyoji1()" style="cursor:hand; cursor:pointer">PCR</h2>
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/* Wrapper for the menu */
 +
.menu_wrapper {
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width:150px;  
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position:fixed;
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text-align:left;
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cursor: pointer;
 +
}
  
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<div id="disp1">
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/* when hovering on a menu item */
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<p>
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.menu_item:hover {
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  Use KOD -Plus- Neo (TOYOBO CO.,LTD) as polymerase. Mix PCR solutions and run the PCR machine in a program which is detailed below.
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color:#000000;  
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</p>
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background-color: #72c9b6;
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<table>
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}
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  <tr>
+
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    <th>Solution</th>
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    <td>template DNA</td>
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    <td>Primer-F 10&micro;M</td>
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    <td>Primer-R 10&micro;M</td>
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    <td>MgSO<sub>4</sub></td>
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    <td>dNTPs</td>
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    <td>10x Buffer</td>
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    <td>KOD Plus Neo</td>
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    <td>DW</td>
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    <td>Total</td>
+
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  </tr>
+
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  <tr>
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    <th>Volume (&micro;L)</th>
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    <td>1</td>
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    <td>1</td>
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    <td>1</td>
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    <td>3</td>
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    <td>5</td>
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    <td>5</td>
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    <td>1</td>
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    <td>33</td>
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    <td>50</td>
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  </tr>
+
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</table>
+
−
<p>Thermal protocol is following</p>
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<h3>2STEP Cycle (Tm value &gt; 63&deg;C)</h3>
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<table>
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  <tr>
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    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (sec)</th>
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  </tr>
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  <tr>
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    <td>1</td><td>94</td><td>120</td>
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  </tr>
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  <tr>
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    <td>2</td><td>98</td><td>10</td>
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−
  </tr>
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  <tr>
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    <td>3</td><td>68</td><td>30sec / 1kbp</td>
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  </tr>
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  <tr>
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    <td>4</td><td>4</td><td>Hold</td>
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  </tr>
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</table>
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<p>Cycle: sequence2~3 &times; (25~45)</p>
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<h3>3STEP Cycle (Tm value &lt; 63&deg;C)</h3>
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/* decoration icon for the menu buttons*/
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<table>
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.icon {
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  <tr>
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float:right; 
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    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (sec)</th>
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font-size:16px;  
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  </tr>
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font-weight:bold;
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  <tr>
+
}
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    <td>1</td><td>94</td><td>120</td>
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  </tr>
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/* this is the icon for when the content is collapsed */
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  <tr>
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.plus::before {
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    <td>2</td><td>98</td><td>10</td>
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content: "+";
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  </tr>
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}
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  <tr>
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/* this is the icon for when the content is expanded */
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    <td>3</td><td>Tm</td><td>30</td>
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.less::before {
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  </tr>
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content: "–";  
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  <tr>
+
}
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    <td>4</td><td>68</td><td>30sec / 1kbp</td>
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−
  </tr>
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  <tr>
+
−
    <td>5</td><td>4</td><td>Hold</td>
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−
  </tr>
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</table>
+
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<p>Cycle: sequence2~4 &times; (25~45)</p>
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 +
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 +
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 +
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 +
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 +
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 +
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 +
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</div>
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  }
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</script>
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/* styling for the submenus */
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.submenu {
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width:100%;
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display: none; 
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font-weight:bold;
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cursor:pointer;
  
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/* moving the margin for the submenu ul list */
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margin: 10px 0px -11px 0px;
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list-style: none;
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<h2 onClick="hyoji2()" style="cursor:hand; cursor:pointer">PCR Purification</h2>
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/*styling for the submenu buttons */
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.submenu li {
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width: 100%;  
 +
margin-left: 10px;
 +
margin-bottom: 0px;
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<div id="disp2">
 
  
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<p>FastGene<sup>TM</sup> Gel/PCR Extraction kit (Nippon Genetics Co., Ltd)
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/* hover state for the submenu buttons */
−
<br>Purification of PCR products</p>
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.submenu li a {
 +
width: 100%;
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padding: 5px 10px;
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display: inline-block;
 +
border-bottom: 1px solid #d3d3d3;
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background-color:white;
 +
text-decoration:none;
 +
color:#000000;
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</div>
 
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<script>
 
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document.getElementById("disp2").style.display="none";
 
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var displaying=0;
 
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function hyoji2()
 
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−
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−
  else
 
−
  {
 
−
    document.getElementById("disp2").style.display="none";
 
−
displaying=0;
 
−
  }
 
−
}
 
−
</script>
 
  
−
<h2 onClick="hyoji3()" style="cursor:hand; cursor:pointer">Digestion</h2>
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.submenu li a:hover  {
−
<div id="disp3">
+
background-color:#000000;  
 +
color: #72c9b6;
 +
}
  
−
<p>Mix the following reagents in PCR tube.</p>
 
−
<table>
 
−
  <tr>
 
−
    <th>Solution</th>
 
−
    <td>DNA</td>
 
−
    <td>RE1 10U/&micro;L</td>
 
−
    <td>RE2 10U/&micro;L</td>
 
−
    <td>Appropriate buffer</td>
 
−
    <td>Total</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <th>Volume (&micro;L)</th>
 
−
    <td>16</td>
 
−
    <td>1</td>
 
−
    <td>1</td>
 
−
    <td>2</td>
 
−
    <td>20</td>
 
−
  </tr>
 
−
</table>
 
−
<table>
 
−
  <tr>
 
−
    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (min)</th>
 
−
  </tr>
 
−
  <tr>
 
−
    <td>1</td><td>37</td><td>120</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td>2</td><td>65</td><td>15</td>
 
−
  </tr>
 
−
  <tr>
 
−
    <td>3</td><td>4</td><td>Hold</td>
 
−
  </tr>
 
−
</table>
 
  
−
</div>
+
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<script>
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−
document.getElementById("disp3").style.display="none";
+
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−
var displaying=0;
+
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−
function hyoji3()
+
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−
{
+
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−
  if (displaying==0)
+
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−
  {
+
font-weight:bold;
−
    document.getElementById("disp3").style.display="block";
+
color:#72c9b6;
−
displaying=1;
+
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−
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+
-webkit-transition: all 0.4s ease;
−
  else
+
-moz-transition: all 0.4s ease;  
−
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+
-ms-transition: all 0.4s ease;  
−
    document.getElementById("disp3").style.display="none";
+
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−
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+
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−
  }
+
}
−
}
+
−
</script>
+
  
−
<h2 onClick="hyoji4()" style="cursor:hand; cursor:pointer">Ligation</h2>
+
/* when hovering on that button */
−
<div id="disp4">
+
.collapsable_menu_control:hover {
−
<p>
+
background-color: #72c9b6;
−
  Mix the following reagents in 0.2 mL PCR tube. Use DNA Ligation Kit &lt;Mighty Mix&amp;rt; (Takara Bio Inc.) which contains ligase and buffer.
+
color:#000000;
−
</p>
+
}
−
<table>
+
−
  <tr>
+
−
    <th>Solution</th><td>Vector DNA</td><td>Insert DNA</td><td>DW</td><td>Mighty Mix</td><td>Total</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <th>Volume (&micro;L)</th><td>1</td><td>2</td><td>2</td><td>5</td><td>10</td>
+
−
  </tr>
+
−
</table>
+
−
<p>Thermal protocol is following</p>
+
−
<table
+
−
  <tr>
+
−
    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (min)</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>1</td><td>16</td><td>30</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>2</td><td>65</td><td>10</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>3</td><td>4</td><td>Hold</td>
+
−
  </tr>
+
−
</table>
+
  
−
</div>
+
/********************************* CONTENT OF THE PAGE ********************************/
−
<script>
+
−
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−
var displaying=0;
+
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+
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−
displaying=1;
+
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  }
+
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  else
+
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+
−
    document.getElementById("disp4").style.display="none";
+
−
displaying=0;
+
−
  }
+
−
}
+
−
</script>
+
  
−
<h2 onClick="hyoji5()" style="cursor:hand; cursor:pointer">Electrophoresis</h2>
+
/* Wrapper for the content */
−
<div id="disp5">
+
.content_wrapper {
−
<ol>
+
width: 85%;
−
  <li>Put gel into electrophoresis tank.</li>
+
margin-left:150px;
−
  <li>Pore 2x TBE buffer into the tank to soak gel.</li>
+
padding:10px 0px;
−
  <li>Add 5  &micro;L  of EtBr into cathod.</li>
+
float:left;
−
  <li>Pre-migration for 30 min at 100 V.</li>
+
background-color:white;
−
  <li>Apply DNA solution with 6x loading dye and ladder.</li>
+
}
−
  <li>Start electrophoresis at 100 V.</li>
+
−
</ol>
+
  
−
</div>
+
/*LAYOUT */
−
<script>
+
.column  {  
−
document.getElementById("disp5").style.display="none";
+
padding: 10px 0px;
−
var displaying=0;
+
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−
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+
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−
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+
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−
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+
−
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+
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+
−
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+
−
  }
+
−
  else
+
−
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+
−
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+
−
displaying=0;
+
−
  }
+
−
}
+
−
</script>
+
  
 +
.full_size {
 +
width:100%;
 +
}
  
−
<h2 onClick="hyoji6()" style="cursor:hand; cursor:pointer">Gel Extraction</h2>
+
.full_size img {
−
<div id="disp6">
+
padding: 10px 15px;
 +
width:96.5%;
 +
}
  
−
<p>FastGene<sup>TM</sup> Gel/PCR Extraction kit (Nippon Genetics Co., Ltd)
+
.half_size {
−
<br>DNA extraction from gel</p>
+
width: 50%;
 +
}
 +
.half_size img {
 +
padding: 10px 15px;
 +
width: 93%;
 +
}
  
−
</div>
 
−
<script>
 
−
document.getElementById("disp6").style.display="none";
 
−
var displaying=0;
 
−
function hyoji6()
 
−
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−
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−
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−
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−
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−
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−
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−
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−
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−
displaying=0;
 
−
  }
 
−
}
 
−
</script>
 
  
 +
.clear {
 +
clear:both;
 +
}
  
−
<h2 onClick="hyoji7()" style="cursor:hand; cursor:pointer">Ethanol precipitation</h2>
+
.highlight {
−
<div id="disp7">
+
width: 90%;  
−
<ol>
+
margin: auto;  
−
  <li>Add 1/10 volume of NaOAc, and 5/2 of 100% ethanol.</li>
+
padding: 15px 5px;
−
  <li>Leave it at room temperature for 5 min.</li>
+
background-color: #f2f2f2;  
−
  <li>Centrifuge at 15,000 rpm for 15 min at 25&deg;C.</li>
+
}
−
  <li>Remove supernatant and add  600 &micro;L  of 70% ethanol.</li>
+
−
  <li>Centrifuge at 15,000 rpm for 5 min at 25&deg;C.</li>
+
−
  <li>Remove supernatant and air-dry at room temperature with light sheilding.</li>
+
−
  <li>Suspend with 10  &micro;L  of TE.</li>
+
−
</ol>
+
  
 +
.judges-will-not-evaluate {
 +
border: 4px solid #72c9b6;
 +
display: block;
 +
margin: 5px 15px;
 +
width: 95%;
 +
font-weight:bold;
 +
}
  
−
</div>
 
−
<script>
 
−
document.getElementById("disp7").style.display="none";
 
−
var displaying=0;
 
−
function hyoji7()
 
−
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−
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−
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−
    document.getElementById("disp7").style.display="block";
 
−
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−
  }
 
−
  else
 
−
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−
    document.getElementById("disp7").style.display="none";
 
−
displaying=0;
 
−
  }
 
−
}
 
−
</script>
 
  
−
<h2 onClick="hyoji8()" style="cursor:hand; cursor:pointer">Colony PCR</h2>
+
/*STYLING */
−
<div id="disp8">
+
−
<table>
+
−
  <tr>
+
−
    <th>Solution</th>
+
−
    <td>DNA</td>
+
−
    <td>Kapa-Taq (Taq polymerase)</td>
+
−
    <td>EX-F primer 10&micro;M</td>
+
−
    <td>PS-R primer 10&micro;M</td>
+
−
    <td>Total</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <th>Volume (&micro;L)</th>
+
−
    <td>4.2</td>
+
−
    <td>5</td>
+
−
    <td>0.4</td>
+
−
    <td>0.4</td>
+
−
    <td>10</td>
+
−
  </tr>
+
−
</table>
+
  
−
<table>
+
/* styling for the titles */
−
  <tr>
+
.content_wrapper h1, .content_wrapper h2 {
−
    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (sec)</th>
+
padding:5px 15px;
−
  </tr>
+
border-bottom:0px;
−
  <tr>
+
color:#72c9b6;
−
    <td>1</td><td>94</td><td>120</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>2</td><td>94</td><td>30</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>3</td><td>68</td><td>60 sec / 1kbp</td>
+
−
  </tr>
+
−
    <td>4</td><td>4</td><td>Hold</td>
+
−
  </tr>
+
−
</table>
+
−
<p>Cycles: sequence2~3 &times; 25~45</p>
+
  
 +
}
 +
.content_wrapper h3, .content_wrapper h4, .content_wrapper h5, .content_wrapper h6 {
 +
padding:5px 15px;
 +
border-bottom:0px;
 +
color: #000000; 
 +
}
  
−
</div>
 
−
<script>
 
−
document.getElementById("disp8").style.display="none";
 
−
var displaying=0;
 
−
function hyoji8()
 
−
{
 
−
  if (displaying==0)
 
−
  {
 
−
    document.getElementById("disp8").style.display="block";
 
−
displaying=1;
 
−
  }
 
−
  else
 
−
  {
 
−
    document.getElementById("disp8").style.display="none";
 
−
displaying=0;
 
−
  }
 
−
}
 
−
</script>
 
  
−
<h2 onClick="hyoji9()" style="cursor:hand; cursor:pointer">Sequencing</h2>
+
/* font and text */
−
<div id="disp9">
+
.content_wrapper p {
−
<table>
+
padding:0px 15px;  
−
  <tr>
+
font-size: 13px;
−
    <th>Solution</th>
+
font-family:Tahoma, Geneva, sans-serif;  
−
    <td>5 x Sequencing Buffer</td>
+
}
−
    <td>primer 1&micro;M</td>
+
−
    <td>template DNA</td>
+
−
    <td>Ready Reaction Premix</td>
+
−
    <td>DW</td>
+
−
    <td>Total</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <th>Volume (&micro;L)</th>
+
−
    <td>1.5</td>
+
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    <td>1.5</td>
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    <td>1</td>
+
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    <td>1</td>
+
−
    <td>5</td>
+
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    <td>10</td>
+
−
  </tr>
+
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</table>
+
  
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+
/* Links */
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    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (sec)</th>
+
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    <td>1</td><td>96</td><td>10</td>
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    <td>2</td><td>50</td><td>5</td>
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    <td>3</td><td>60</td><td>240</td>
+
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  </tr>
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  <tr>
+
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    <td>4</td><td>4</td><td>Hold</td>
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  </tr>
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</table>
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<p>Cycle: sequence2~4 &times; 25</p>
+
  
 +
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<h3>Ethanol precipitation</h3>
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    <th>Solution</th>
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    <td>3M NaOAc</td>
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    <td>Glycogen</td>
+
−
    <td>100% EtOH</td>
+
−
  </tr>
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−
  <tr>
+
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    <th>Volume (&micro;L)</th>
+
−
    <td>10</td>
+
−
    <td>10</td>
+
−
    <td>2</td>
+
−
    <td>1</td>
+
−
    <td>50</td>
+
−
  </tr>
+
−
</table>
+
−
<ol>
+
−
  <li>Centrifuge at 15,000 rpm for 15 min at room temprature</li>
+
−
  <li>Remove supernatant ,add 100 &micro;L  of 70% EtOH and tap tubes by finger.</li>
+
−
  <li>Centrifuge at 15,000 rpm for 10 min at room temprature</li>
+
−
  <li>Remove supernatant and air dry at room temperature.</li>
+
−
  <li>Resuspend the pellet to HiDi formamide and remove to 96-well plate.</li>
+
−
  <li>Set the plate and start electrophoresis.</li>
+
−
</ol>
+
  
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 +
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<li>Thaw original competent cells on ice.</li>
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−
<li>Add 5 &micro;L of original competent cells to 2 mL of LB.</li>
+
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+
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−
<li>Add 5 &micro;L, 50 &micro;L, and 500 &micro;L of original cells to 100 mL of LB.</li>
+
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−
<li>Incubate the cells at 130 rpm at 20&deg;C, until OD<sub>600</sub> reach 0.5.</li>
+
−
<li>Take 50 mL of incubated cells to two differnt culture tubes and centrifuge them at 3,000 rpm for 20 min at 4&deg;C.</li>
+
−
<li>Remove supernatant and add 75 mL of TB to each tube.</li>
+
−
<li>Bring them to a one tube and centrifuge at 3,000 rpm for 20 min at 4&deg;C.</li>
+
−
<li>Remove supernatant and add 32 mL of TB.</li>
+
−
<li>Add 32 &micro;L of DMSO 10 times.</li>
+
−
<li>Take 50 &micro;L and freeze with liquid nitrogen.</li>
+
−
</ol>
+
  
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<h2 onClick="hyoji11()" style="cursor:hand; cursor:pointer">Transformation</h2>
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/* Button class */
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<ol>
+
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−
  <li>Add plasmid to thawed competent cells on ice.</li>
+
padding: 15px; width:12%; 
−
  <li>Incubate on ice for 30 min.</li>
+
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−
  <li>Add to LB.</li>
+
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−
  <li>(Incubate the cells for 2 hrs at 37&deg;C.)</li>
+
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−
  <li>Spread 300  &micro;L  of the culture onto plate with LB and appropriate antibiotics.</li>
+
cursor:pointer; 
−
  <li>Incubate the plate(s) at 37&deg;C for 16~20 hrs.</li>
+
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−
</ol>
+
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 +
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 +
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 +
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 +
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−
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+
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−
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+
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+
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+
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+
−
</script>
+
  
−
<h2 onClick="hyoji12()" style="cursor:hand; cursor:pointer">Mini-prep</h2>
+
/********************************* RESPONSIVE STYLING ********************************/
−
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+
−
<p>FastGene<sup>TM</sup> Plasmid mini kit (Nippon Genetics Co., Ltd)
+
−
<br>fast / standard / low copy protocol</p>
+
  
−
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+
/* IF THE SCREEN IS LESS THAN 1000PX */
−
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+
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+
  
−
<h2 onClick="hyoji13()" style="cursor:hand; cursor:pointer">Streaking (Single colony isolation)</h2>
+
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−
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+
−
<ol>
+
−
  <li>Pick the colony with an inoculating loop from the agar plate．</li>
+
−
  <li>Drag the loop across on a new agar plate.</li>
+
−
  <li>Re-sterilise the loop and drag it across again.</li>
+
−
  <li>Repeat method 3.</li>
+
−
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+
  
−
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+
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−
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+
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−
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+
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+
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+
  
−
<h2 onClick="hyoji14()" style="cursor:hand; cursor:pointer">PEG precipitation</h2>
 
−
<div id="disp14">
 
−
<ol>
 
−
  <li>Add 13  &micro;L  of PEG to 20  &micro;L  of product(s).</li>
 
−
  <li>Leave it at room temperature for 1 hr.</li>
 
−
  <li>Centrifuge at 15,000 rpm for 20 min at 4&deg;C.</li>
 
−
  <li>Remove supernatant and add  100 &micro;L  of 70% ethanol.</li>
 
−
  <li>Centrifuge at 15,000 rpm for 2 min at 4&deg;C.</li>
 
−
  <li>Remove supernatant and air-dry at room temperature with light sheilding.</li>
 
−
  <li>Suspend with 10  &micro;L  of TE.</li>
 
−
</ol>
 
  
−
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+
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−
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+
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+
−
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+
  
−
<h2 onClick="hyoji15()" style="cursor:hand; cursor:pointer">Gel Free System</h2>
+
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−
<div id="disp15">
+
.collapsable_menu_control { display:block;}
−
<h3>Preparation of biotinylated DNA fragments</h3>
+
.menu_item {display:none;}
−
<p>
+
.menu_wrapper { width:100%; height: 15%; position:relative;}
−
  Use KOD -Plus- Neo (TOYOBO CO.,LTD) as polymerase and 5'-biotinylated primers. Mix PCR solutions and run the PCR machine in a program which is detailed below.
+
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−
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+
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−
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+
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−
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+
.icon {display:block;}
−
    <th>Solution</th>
+
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−
    <td>template DNA</td>
+
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−
    <td>5'-biotinylated 100-UP primer 10&micro;M</td>
+
−
    <td>5'-biotinylated 200-DN primer 10&micro;M</td>
+
−
    <td>MgSO<sub>4</sub></td>
+
−
    <td>dNTPs</td>
+
−
    <td>10x Buffer</td>
+
−
    <td>KOD Plus Neo</td>
+
−
    <td>DW</td>
+
−
    <td>Total</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <th>Volume (&micro;L)</th>
+
−
    <td>1</td>
+
−
    <td>1.5</td>
+
−
    <td>1.5</td>
+
−
    <td>3</td>
+
−
    <td>5</td>
+
−
    <td>5</td>
+
−
    <td>1</td>
+
−
    <td>32</td>
+
−
    <td>50</td>
+
−
  </tr>
+
−
</table>
+
−
<p>Thermal protocol is following</p>
+
−
<h3>2STEP Cycle (Tm value &gt; 63&deg;C)</h3>
+
−
<table>
+
−
  <tr>
+
−
    <th>Sequence</th><th>Temp. (&deg;C)</th><th>Time (sec)</th>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>1</td><td>94</td><td>120</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>2</td><td>98</td><td>10</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>3</td><td>68</td><td>30sec / 1kbp</td>
+
−
  </tr>
+
−
  <tr>
+
−
    <td>4</td><td>4</td><td>Hold</td>
+
−
  </tr>
+
−
</table>
+
−
<p>Cycle: sequence2~3 &times; (25~45)</p>
+
  
−
<h3>Preparation of magnetic beads</h3>
+
</style>
−
<ol>
+
−
  <li>Mix 2 &micro;L  of magnetic beads (SiMAG-Streptavidin) and 48 &micro;L  of TE by vibration using sonic-toothbrush.</li>
+
−
  <li>Collect the beads by attracting them to one side in 0.2 mL  polypropylene tube using neodymium magnet.</li>
+
−
  <li>Remove supernatant.</li>
+
−
</ol>
+
  
−
<h3>Fixation to magnetic beads</h3>
 
−
<ol>
 
−
  <li>Add 3 &micro;L  of PCR product (0.48 pmol) and 7 &micro;L  of TE to beads.</li>
 
−
  <li>Mix by vibration using sonic-toothbrush.</li>
 
−
  <li>Collect the beads using magnet.</li>
 
−
  <li>Remove supernatant containing excess amount of free DNA fragment.</li>
 
−
</ol>
 
  
−
<h3>Double restriction digestion</h3>
+
 
−
<ol>
+
 
−
   <li>Add Digestion Premix containing 1 &micro;L of 10x RE solution, 8 &micro;L of DW and each 0.5 &micro;L of restriction endonuclease, <I>Xba</I>I and <I>Spe</I>I, to the beads.</li>
+
<!--- THIS IS WHERE THE HTML BEGINS --->
−
   <li>Mix by pumping using pipette.</li>
+
 
−
   <li>Incubate at 37 &deg;C for 30 min.</li>
+
 
−
   <li>Collect the beads using magnet.</li>
+
<!-- This tells the browser that your page is responsive -->
−
   <li>Obtain supernatant containing digested DNA fragment.</li>
+
 
−
   <li>Purify the supernatant by ethanol precipitation.</li>
+
<head>
−
</ol>
+
<meta name="viewport" content="width=device-width, initial-scale=1">
 +
</head>
 +
 
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 +
<h1 id="team_name">  </h1>
 +
<h4 id="page_name">  </h4>
 +
 +
 +
 +
 +
 +
 +
 +
 +
 
<script>
 
<script>
−
document.getElementById("disp15").style.display="none";
+
 
−
var displaying=0;
+
// This is the jquery part of your template.  Try not modify any of this code since it makes your menu work.
−
function hyoji15()
+
 
−
{
+
$(document).ready(function() {
−
  if (displaying==0)
+
 
−
  {
+
$("#HQ_page").attr('id','');
−
     document.getElementById("disp15").style.display="block";
+
−
displaying=1;
+
 
−
  }
+
if ( wgPageName.substring( 0,  8) == "Template")  {  // if the page is a template it displays the full name in a single line
−
  else
+
$("#team_name").html( wgPageName );
−
  {
+
}
−
    document.getElementById("disp15").style.display="none";
+
 
−
displaying=0;
+
else if ( ( (wgPageName.match(/\//g) || []).length ) == 0 ) {  // if it is the home page , just print the team's name
−
  }
+
$("#team_name").html( wgPageName.substring( 5, wgPageName.length ) );
−
}
+
}
 +
 
 +
else {
 +
// this adds the team's name as an h1
 +
$("#team_name").html( wgPageName.substring( 5 , wgPageName.indexOf("/")  ) );
 +
 
 +
// this adds the page's title as an h4
 +
$("#page_name").html (     ( wgPageName.substring( wgPageName.indexOf("/") + 1, wgPageName.length ) ).replace( /\/|_/g , " ")  );
 +
}
 +
 
 +
 
 +
 
 +
 
 +
$('#accordion').find('.menu_item').click(function(){
 +
 
 +
//Expand or collapse this panel
 +
submenu = $(this).find('.submenu');
 +
submenu.toggle();
 +
 
 +
icon = $(this).find('.icon');
 +
 
 +
if ( !$( submenu ).is(':visible') ) {
 +
icon.removeClass("less").addClass("plus");
 +
}
 +
else {
 +
icon.removeClass("plus").addClass("less");
 +
}
 +
 
 +
//Hide the other panels
 +
$(".submenu").not(submenu).hide();
 +
$(".icon").not(icon).removeClass("less").addClass("plus");
 +
});
 +
 
 +
 
 +
$(".collapsable_menu_control").click(function() {
 +
$(".menu_item").toggle();
 +
});
 +
 
 +
$( window ).resize(function() {
 +
$(".menu_item").show();
 +
});
 +
 
 +
 
 +
});
 +
 
 +
 
 +
 
 +
 
 
</script>
 
</script>
 +
  
 
</html>
 
</html>

Revision as of 05:15, 26 August 2016

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