Difference between revisions of "Team:Paris Saclay/Notebook/August/26"

(→‎Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product)
(→‎Friday 26th August)
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{{Team:Paris_Saclay/notebook_header}}
 
{{Team:Paris_Saclay/notebook_header}}
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= Friday 26<sup>th</sup> August=
 
= Friday 26<sup>th</sup> August=
 
==Lab work==
 
==Lab work==
 
===Visualization===
 
===Visualization===
 +
====Purification of pPS16-003 and pPS16_004 ligation products====
 +
''By Mathilde''
 +
 +
Ligation products pPS16-003 and pPS16_004 frome the [[https://2016.igem.org/Team:Paris_Saclay/Notebook/August/25#Visualization|day before]] were purificated according to the usual [[https://2016.igem.org/Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]]
 +
 +
 
====Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product====
 
====Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product====
 
'' By Mathilde ''
 
'' By Mathilde ''
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[[File:T--Paris_Saclay--160826_gel_ligation_fragm2.png|400px|thumb|centre|Migration Results]]
 
[[File:T--Paris_Saclay--160826_gel_ligation_fragm2.png|400px|thumb|centre|Migration Results]]
 +
 +
The two bands are at the expected size (1860 pb), so the fragment 2 (16-003 and pPS16_004 ligation product) is purified.
 +
 +
==== Purification of fragments 1(16-001 + pPS16_002) and 2 (16-003 + pPS16_004)====
 +
'' By Mathilde''
 +
 +
The purification was made following the usual [[https://2016.igem.org/Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up|protocol]].
 +
Each of those purification products were quantified on nanodrop.
 +
 +
{| class="wikitable"
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|-
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!Segment
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!DNA quantity (ng/µL)
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!260/230
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!260/280
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|-
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|1
 +
|2
 +
|-
 +
|84,20
 +
|1,25
 +
|1,84
 +
|-
 +
|169,88
 +
|1,29
 +
|1,83
 +
|}
 +
  
 
{{Team:Paris_Saclay/notebook_footer}}
 
{{Team:Paris_Saclay/notebook_footer}}

Revision as of 13:53, 10 September 2016


Friday 26th August

Lab work

Visualization

Purification of pPS16-003 and pPS16_004 ligation products

By Mathilde

Ligation products pPS16-003 and pPS16_004 frome the [before] were purificated according to the usual [[1]]


Q5 high fidelity PCR of pPS16_003 - pPS16_004 ligation product

By Mathilde

The Q5 PCR was conducted following the exact same protocol than the 25/08/2016 on the ligation product from the day before.

Migration Results

The two bands are at the expected size (1860 pb), so the fragment 2 (16-003 and pPS16_004 ligation product) is purified.

Purification of fragments 1(16-001 + pPS16_002) and 2 (16-003 + pPS16_004)

By Mathilde

The purification was made following the usual [[2]]. Each of those purification products were quantified on nanodrop.

Segment DNA quantity (ng/µL) 260/230 260/280
1 2
84,20 1,25 1,84
169,88 1,29 1,83






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