This question is essential because the distance between the dCas9 may cause major problem: if this system doesn’t work we may not see the effect of the other system. In fact, the 3D structure of our linker may interact with the dCas9 or the GFP because of steric hindrance.
This question is essential because the distance between the dCas9 may cause major problem: if this system doesn’t work we may not see the effect of the other system. In fact, the 3D structure of our linker may interact with the dCas9 or the GFP because of steric hindrance.