Revision as of 13:55, 10 September 2016

Friday 26^th August

By Mathilde

Ligation products pPS16-003 and pPS16_004 frome the day before were purificated according to the usual protocol

By Mathilde

The Q5 PCR was conducted following the exact same protocol than the 25/08/2016 on the ligation product from the day before.

Migration Results

The two bands are at the expected size (1860 pb), so the fragment 2 (16-003 and pPS16_004 ligation product) is purified.

By Mathilde

The purification was made following the usual [[1]]. Each of those purification products were quantified on nanodrop.

Segment	DNA quantity (ng/µL)	260/230
1	2
84,20	1,25	1,84
169,88	1,29	1,83

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	''By Mathilde''		''By Mathilde''

−	Ligation products pPS16-003 and pPS16_004 frome the [[~~https://2016.igem.org/~~Team:Paris_Saclay/Notebook/August/25#Visualization\|day before]] were purificated according to the usual [[~~https://2016.igem.org/~~Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up\|protocol]]	+	Ligation products pPS16-003 and pPS16_004 frome the [[Team:Paris_Saclay/Notebook/August/25#Visualization\|day before]] were purificated according to the usual [[Team:Paris_Saclay/Experiments#Purification_:_PCR_clean-up_with_NucleoSpin_Gel_and_PCR_Clean-up\|protocol]]