Difference between revisions of "Team:Paris Saclay/Safety"

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<p>Introduction
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<p>Safety is a crucial issue in iGEM projects. As students imagine their own project, they have to make sure that their idea is not hazardous. Likewise, realization of the project in the lab is a second challenge for young students in terms of security. Working on living organisms and biological parts requires good laboratory practices in order to prevent spreading, contamination, or physical injuries.
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<p>A good laboratory practice:
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<p>To prevent disorder and accidents, the lab was divided in two parts : a wet laboratory for experiments and a dry laboratory for personal working. Students who were performing experiments were always wearing lab coats and kept experiment materials in the wet laboratory.
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<p>Chemical product handling :
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<p>Students who were experimenting sometimes had to use dangerous chemical products. Extra precautions were taken to handle them :
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{| class="wikitable"
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|-
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!Products
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!Dangers
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!Precautions
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!Usages
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|-
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|EtBr (Ethidium bromide)
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|<p>mutagen agent
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<p>cancer-causing agent
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|<p>always manipulate EtBr wearing gloves
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<p>add EtBr in cool solution only to prevent inhalation
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<p>set up a special place and gather material to manipulate EtBr only
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|DNA visualization in agarose gels
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|-
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|Phenol/Chloroform
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|mutagen agent
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<p>toxic agent
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<p>irritant agent
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<p>cancer-causing agent
 +
|always manipulate Phenol/Chloroform wearing gloves under a hood
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|DNA extraction
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|}
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<p>Please visit <a href="https://2016.igem.org/Safety">the main Safety page</a> to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.</p>
 
  
 
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>
 
<p>On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can <strong>go beyond the questions on the safety forms</strong>, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)</p>

Revision as of 14:45, 12 September 2016

Introduction

Safety is a crucial issue in iGEM projects. As students imagine their own project, they have to make sure that their idea is not hazardous. Likewise, realization of the project in the lab is a second challenge for young students in terms of security. Working on living organisms and biological parts requires good laboratory practices in order to prevent spreading, contamination, or physical injuries.

A good laboratory practice:

To prevent disorder and accidents, the lab was divided in two parts : a wet laboratory for experiments and a dry laboratory for personal working. Students who were performing experiments were always wearing lab coats and kept experiment materials in the wet laboratory.

Chemical product handling :

Students who were experimenting sometimes had to use dangerous chemical products. Extra precautions were taken to handle them : {| class="wikitable" |- !Products !Dangers !Precautions !Usages |- |EtBr (Ethidium bromide) |

mutagen agent

cancer-causing agent |

always manipulate EtBr wearing gloves

add EtBr in cool solution only to prevent inhalation

set up a special place and gather material to manipulate EtBr only |DNA visualization in agarose gels |- |Phenol/Chloroform |mutagen agent

toxic agent

irritant agent

cancer-causing agent |always manipulate Phenol/Chloroform wearing gloves under a hood |DNA extraction |}

On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)

Safe Project Design

Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:

  • Choosing a non-pathogenic chassis
  • Choosing parts that will not harm humans / animals / plants
  • Substituting safer materials for dangerous materials in a proof-of-concept experiment
  • Including an "induced lethality" or "kill-switch" device
Safe Lab Work

What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?